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The map label for this gene is liaR [H]

Identifier: 17547880

GI number: 17547880

Start: 3417459

End: 3418154

Strand: Reverse

Name: liaR [H]

Synonym: RSc3161

Alternate gene names: 17547880

Gene position: 3418154-3417459 (Counterclockwise)

Preceding gene: 17547886

Following gene: 17547879

Centisome position: 91.97

GC content: 64.94

Gene sequence:

>696_bases
ATGTGTCCCTCCAGTACCGCTCCGGAACTGCCATCCATCCGCGTCGCGATCGTCGAGGACGATTCCGGCTTTCTCGATGC
CTTGACCCAGGCCCTGGCGTGCGCGCCGGATATGCACTTGACGGGCGTCGCCGGCTCCCGTGCCGAAGGCCTGCTGCTGC
TCGATGGCGCGCCGGCGGATGTGCTGCTGGTCGACCTTGGGTTGCCTGACGGCTCGGGCATCGACGTGATCGAAGCTGCC
GCCCGGAAATGGAGCGCTTGCAGCATCATGGTCAGTACCAATTTCGGCGACGAAATCCACGTGATGCGTTCGATCGAGGT
CGGCGCGGCCGGCTATCTGCTCAAGGACAGTTCAGCGGCCAGAATCCTCGACGAAATCCGCAGCCTCGCCAGCGGCGGCA
GCCCGATCAGCCCGCTCATCGCCCGGCAGATCCTGGCGCGCTTCCGGCAAGGCGGCTCCCTCAGCGCGGCAGACGAGGCG
CCCGCTTCGGCGTCTTCGCCCCTGCTGTCGGCGCGTGAAAAAGAGGTGCTGGACCTGATCACCAAGGGCTTCACAGCGCA
GGAGATCGCCAAGCTGATGGCGTTGTCCCATTTCACGGTGCGGACCTTCGTGCGGCGCATCTACAGCAAGCTCAAGGTCA
CCTCCAAGGCCGAAGCCATTTATGAGGCCCGGACGCTGGGGCTGTTGTCCGACTAG

Upstream 100 bases:

>100_bases
TTTTTGCAAGCGCGCCGAGTATATTCACGGGGCTTGATCCCATCTGTCGCACAAATGCGCTACACGGGCCGTCCCCCCGC
CGCCCTTAGAACGACGGTCC

Downstream 100 bases:

>100_bases
GCAGGGCTTGCCCGCATTTCCTACTGAACAGCCGGCTTTTTCGAGCATGGCGATATCGGCGCCGCATGAGATACCGCCCC
ACACACCTTCCCGCCTACGC

Product: two component response regulator transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MCPSSTAPELPSIRVAIVEDDSGFLDALTQALACAPDMHLTGVAGSRAEGLLLLDGAPADVLLVDLGLPDGSGIDVIEAA
ARKWSACSIMVSTNFGDEIHVMRSIEVGAAGYLLKDSSAARILDEIRSLASGGSPISPLIARQILARFRQGGSLSAADEA
PASASSPLLSAREKEVLDLITKGFTAQEIAKLMALSHFTVRTFVRRIYSKLKVTSKAEAIYEARTLGLLSD

Sequences:

>Translated_231_residues
MCPSSTAPELPSIRVAIVEDDSGFLDALTQALACAPDMHLTGVAGSRAEGLLLLDGAPADVLLVDLGLPDGSGIDVIEAA
ARKWSACSIMVSTNFGDEIHVMRSIEVGAAGYLLKDSSAARILDEIRSLASGGSPISPLIARQILARFRQGGSLSAADEA
PASASSPLLSAREKEVLDLITKGFTAQEIAKLMALSHFTVRTFVRRIYSKLKVTSKAEAIYEARTLGLLSD
>Mature_231_residues
MCPSSTAPELPSIRVAIVEDDSGFLDALTQALACAPDMHLTGVAGSRAEGLLLLDGAPADVLLVDLGLPDGSGIDVIEAA
ARKWSACSIMVSTNFGDEIHVMRSIEVGAAGYLLKDSSAARILDEIRSLASGGSPISPLIARQILARFRQGGSLSAADEA
PASASSPLLSAREKEVLDLITKGFTAQEIAKLMALSHFTVRTFVRRIYSKLKVTSKAEAIYEARTLGLLSD

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=212, Percent_Identity=30.188679245283, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788222, Length=173, Percent_Identity=30.635838150289, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790102, Length=206, Percent_Identity=26.2135922330097, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24253; Mature: 24253

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCPSSTAPELPSIRVAIVEDDSGFLDALTQALACAPDMHLTGVAGSRAEGLLLLDGAPAD
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCCC
VLLVDLGLPDGSGIDVIEAAARKWSACSIMVSTNFGDEIHVMRSIEVGAAGYLLKDSSAA
EEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCCEEEECCHHH
RILDEIRSLASGGSPISPLIARQILARFRQGGSLSAADEAPASASSPLLSAREKEVLDLI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TKGFTAQEIAKLMALSHFTVRTFVRRIYSKLKVTSKAEAIYEARTLGLLSD
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCPSSTAPELPSIRVAIVEDDSGFLDALTQALACAPDMHLTGVAGSRAEGLLLLDGAPAD
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCCC
VLLVDLGLPDGSGIDVIEAAARKWSACSIMVSTNFGDEIHVMRSIEVGAAGYLLKDSSAA
EEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCCEEEECCHHH
RILDEIRSLASGGSPISPLIARQILARFRQGGSLSAADEAPASASSPLLSAREKEVLDLI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TKGFTAQEIAKLMALSHFTVRTFVRRIYSKLKVTSKAEAIYEARTLGLLSD
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]