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The map label for this gene is cpg2 [H]

Identifier: 17547812

GI number: 17547812

Start: 3330055

End: 3331365

Strand: Reverse

Name: cpg2 [H]

Synonym: RSc3093

Alternate gene names: 17547812

Gene position: 3331365-3330055 (Counterclockwise)

Preceding gene: 17547815

Following gene: 17547810

Centisome position: 89.64

GC content: 68.27

Gene sequence:

>1311_bases
ATGAGAGCCATGTCGTTCGCGCGGGGAGTGCGCCCGGTCATGCTGTCCTTGCTGGTGGCGGCCGGCGCGCTGGCCGCGGG
CCAGGCGGCCGCCGTGCCGCCGGCCGAGATCTACAAGCGCGCCCAGCAGGAGAAGCCGGCCCTGATCGACACCATGAAGA
CGCTGGTGTCGATTGAATCGGGCAGCAGGGACCTTGAGGGCCTGGACAGGATCGCAGGCGTGATCGCCGACCGCCTGCGC
CTGCTGGGCGGTGACGTGAAGCTGGTCGACCCCACCGACCACGCCTACCGCATGGCCGACACCCCCGAGAAGATCGGCAA
GATGGTGCTGGCGCGCTTCAAGGGCGAGGGCAAGCGCAACATCATGCTGATCGCGCACATGGACACGGTGTACCTCAAGG
GCATGCTGGCGCAGCAGCCGTTCCGCATCGACGGCGACCGCGCCTACGGTCTGGGCATTGCCGACGACAAGAACGGCGTG
GCGGTGATCCTGCACACGGTGTCCATCCTGCAGTCGGTCGGCTTCAAGCAGTACGGCACGCTGACCGTGCTGATCAACGG
CGACGAAGAAATCAGCTCGCCCGGCGCACGCACCCTGCTGGCCAAGCTGGGCGCCGAGCAGGATGCGGTGTTCTCGTGCG
AGGGCACGCGTGCCAGCACCGACAAGCTGTCGCTTGCCACCAGCGGCATCGGCGCGATCCTGCTGGATGTGAAGGGCAAG
GCATCCCATGCGGGCGGCGCACCGGAGCAGGGCCGCAACGCCCTGTATGAACTGTCTCACCAGGTGCTGCAACTGCGCGA
CCTGTCCAACCCGCAGACGGGGCTGAAGGTCAACTGGACACTGGCCCAGGCCGGCACCAACCGCAACGTGATCCCCGCCA
CCGCGAGCGCCCAGGCCGATGTGCGCCTGCTGCGCGCGGCCGACGCCGACAAGCTGGAAGACACCATCAACGAGCGCATC
AAGACCCGGCTGATCCCCGATACGCTGGTCACGGCCCGGTTCGAACGCCGCCGGCCGCCGCTGGAGGCGACGCCGGCGTC
GCATCGTCTCGGCGAACATGCGCAGAAGATCTACGGCGAACTCGGCAAGTCGCTTGAGATCGACGACAAGGCGGAGGGCG
GCGGCACCGACGCCGCATTCGCCGCCTCCAGGACACAGGCGCCGGTTCTCGAGCGCTTCGGCCTGGCCGGCGCCGGCGCG
CACTCGAACGAGGCGGAATACGTCGACCTGAACTCCATCGTGCCGCGCCTGTATCTGCTGTCGCGCATGGTCATGGACGT
CTCGCGCGACCGCGTGGGCGGCGCCCGGTAA

Upstream 100 bases:

>100_bases
TCCGAACAGGGGATGGCACCGCCGATGGCCCCCGGGCCGCCGTGCGAAAATCGGTGCTTTGTTGCAAACCATAACTAAGA
GAACAGGTGAGGAAACTTCC

Downstream 100 bases:

>100_bases
CGCTGCGACGAGGCGGCACACGGGGCCCGCCCATGCGCGCCGCCGATCACGCCGGACAAGTCTCCGCGATCGGCTCCCAT
GAAAAAGCCCTGCCGGCGCA

Product: glutamate carboxypeptidase

Products: NA

Alternate protein names: CPDG2; Folate hydrolase G2; Glutamate carboxypeptidase; Pteroylmonoglutamic acid hydrolase G2; Glucarpidase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MRAMSFARGVRPVMLSLLVAAGALAAGQAAAVPPAEIYKRAQQEKPALIDTMKTLVSIESGSRDLEGLDRIAGVIADRLR
LLGGDVKLVDPTDHAYRMADTPEKIGKMVLARFKGEGKRNIMLIAHMDTVYLKGMLAQQPFRIDGDRAYGLGIADDKNGV
AVILHTVSILQSVGFKQYGTLTVLINGDEEISSPGARTLLAKLGAEQDAVFSCEGTRASTDKLSLATSGIGAILLDVKGK
ASHAGGAPEQGRNALYELSHQVLQLRDLSNPQTGLKVNWTLAQAGTNRNVIPATASAQADVRLLRAADADKLEDTINERI
KTRLIPDTLVTARFERRRPPLEATPASHRLGEHAQKIYGELGKSLEIDDKAEGGGTDAAFAASRTQAPVLERFGLAGAGA
HSNEAEYVDLNSIVPRLYLLSRMVMDVSRDRVGGAR

Sequences:

>Translated_436_residues
MRAMSFARGVRPVMLSLLVAAGALAAGQAAAVPPAEIYKRAQQEKPALIDTMKTLVSIESGSRDLEGLDRIAGVIADRLR
LLGGDVKLVDPTDHAYRMADTPEKIGKMVLARFKGEGKRNIMLIAHMDTVYLKGMLAQQPFRIDGDRAYGLGIADDKNGV
AVILHTVSILQSVGFKQYGTLTVLINGDEEISSPGARTLLAKLGAEQDAVFSCEGTRASTDKLSLATSGIGAILLDVKGK
ASHAGGAPEQGRNALYELSHQVLQLRDLSNPQTGLKVNWTLAQAGTNRNVIPATASAQADVRLLRAADADKLEDTINERI
KTRLIPDTLVTARFERRRPPLEATPASHRLGEHAQKIYGELGKSLEIDDKAEGGGTDAAFAASRTQAPVLERFGLAGAGA
HSNEAEYVDLNSIVPRLYLLSRMVMDVSRDRVGGAR
>Mature_436_residues
MRAMSFARGVRPVMLSLLVAAGALAAGQAAAVPPAEIYKRAQQEKPALIDTMKTLVSIESGSRDLEGLDRIAGVIADRLR
LLGGDVKLVDPTDHAYRMADTPEKIGKMVLARFKGEGKRNIMLIAHMDTVYLKGMLAQQPFRIDGDRAYGLGIADDKNGV
AVILHTVSILQSVGFKQYGTLTVLINGDEEISSPGARTLLAKLGAEQDAVFSCEGTRASTDKLSLATSGIGAILLDVKGK
ASHAGGAPEQGRNALYELSHQVLQLRDLSNPQTGLKVNWTLAQAGTNRNVIPATASAQADVRLLRAADADKLEDTINERI
KTRLIPDTLVTARFERRRPPLEATPASHRLGEHAQKIYGELGKSLEIDDKAEGGGTDAAFAASRTQAPVLERFGLAGAGA
HSNEAEYVDLNSIVPRLYLLSRMVMDVSRDRVGGAR

Specific function: Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity [H]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=410, Percent_Identity=26.3414634146341, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR017150
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.17.11 [H]

Molecular weight: Translated: 46673; Mature: 46673

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAMSFARGVRPVMLSLLVAAGALAAGQAAAVPPAEIYKRAQQEKPALIDTMKTLVSIES
CCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
GSRDLEGLDRIAGVIADRLRLLGGDVKLVDPTDHAYRMADTPEKIGKMVLARFKGEGKRN
CCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECCCCHHHHHHHHHHHHCCCCCCE
IMLIAHMDTVYLKGMLAQQPFRIDGDRAYGLGIADDKNGVAVILHTVSILQSVGFKQYGT
EEEEEECHHHHHHHHHHCCCEEECCCCEEECCCCCCCCCCEEHHHHHHHHHHCCCCCCCE
LTVLINGDEEISSPGARTLLAKLGAEQDAVFSCEGTRASTDKLSLATSGIGAILLDVKGK
EEEEECCCHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHCCCEEEEEEECCC
ASHAGGAPEQGRNALYELSHQVLQLRDLSNPQTGLKVNWTLAQAGTNRNVIPATASAQAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEECCCCCCHHH
VRLLRAADADKLEDTINERIKTRLIPDTLVTARFERRRPPLEATPASHRLGEHAQKIYGE
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LGKSLEIDDKAEGGGTDAAFAASRTQAPVLERFGLAGAGAHSNEAEYVDLNSIVPRLYLL
HCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHH
SRMVMDVSRDRVGGAR
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRAMSFARGVRPVMLSLLVAAGALAAGQAAAVPPAEIYKRAQQEKPALIDTMKTLVSIES
CCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
GSRDLEGLDRIAGVIADRLRLLGGDVKLVDPTDHAYRMADTPEKIGKMVLARFKGEGKRN
CCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECCCCHHHHHHHHHHHHCCCCCCE
IMLIAHMDTVYLKGMLAQQPFRIDGDRAYGLGIADDKNGVAVILHTVSILQSVGFKQYGT
EEEEEECHHHHHHHHHHCCCEEECCCCEEECCCCCCCCCCEEHHHHHHHHHHCCCCCCCE
LTVLINGDEEISSPGARTLLAKLGAEQDAVFSCEGTRASTDKLSLATSGIGAILLDVKGK
EEEEECCCHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHCCCEEEEEEECCC
ASHAGGAPEQGRNALYELSHQVLQLRDLSNPQTGLKVNWTLAQAGTNRNVIPATASAQAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEECCCCCCHHH
VRLLRAADADKLEDTINERIKTRLIPDTLVTARFERRRPPLEATPASHRLGEHAQKIYGE
HHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LGKSLEIDDKAEGGGTDAAFAASRTQAPVLERFGLAGAGAHSNEAEYVDLNSIVPRLYLL
HCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHH
SRMVMDVSRDRVGGAR
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6396165; 3838935; 9083113 [H]