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The map label for this gene is hemB [H]

Identifier: 17547708

GI number: 17547708

Start: 3222187

End: 3223182

Strand: Direct

Name: hemB [H]

Synonym: RSc2989

Alternate gene names: 17547708

Gene position: 3222187-3223182 (Clockwise)

Preceding gene: 17547707

Following gene: 17547764

Centisome position: 86.7

GC content: 67.57

Gene sequence:

>996_bases
ATGATCGCAAGCTTCCCCGACCACCGCCCGCGTCGCATGCGCCGCGACGACTTCTCCCGCCGGATGATGCGCGAATCGCG
CCTGACGGCCGACGACCTGATCTATCCGGTCTTCATCCTGGAAGGCACCAACGTACGCCAGGCCGTGCCGTCGATGCCGG
GCGTCGAGCGCGTCTCCGTCGACGTGCTGCTGGGCGTGGCCGAGCAATGCGTGCAGCTCGGGATTCCCGTGCTGGCGCTC
TTCCCCGTCATCGAGCCGAGCCTGAAGACGCCGGACGGCATCGAAGCCACCAACGCCGATGGGCTGATCCCCCGCGCGGT
CAAGGCGCTCAAGGCGCGCTTTCCGGAACTCGGCATCCTCACCGACGTGGCGCTCGACCCGTACACCAGCCATGGCCAGG
ACGGCGTGCTCGACGATGCCGGCTACGTGCTCAACGAAGAAACAGTCGAGATCCTGACGCGCCAGGCGCTGGTGCAGGCC
GAGGCCGGGGTGGACATCGTCGCGCCGTCGGACATGATGGACGGCCGCATCGGCGCGATCCGCCTGGCGCTGGAGAACGA
CGACCACATCTACACACGCATCATGGCCTACGCGGCCAAGTATGCGTCGGCGTTCTACGGCCCGTTCCGCGACGCGGTGG
GCTCGGCGGCCAACCTGGGCAAGGGCAACAAGATGACCTACCAGATGGACCCGGCCAACTCCGACGAGGCGCTGCGCGAA
GTGGCCCTCGACATCGCCGAGGGCGCCGACATGGTGATGGTCAAGCCGGGCATGCCGTACCTCGACATCGTGCGCCGCGT
GAAGGACGAGTTCCGTTTCCCGACCTACGTCTACCAGGTCAGCGGCGAGTACGCGATGCTCAAGGCTGCCGCCCAGAACG
GCTGGCTGGACCACGACAAGGTCGTGCTGGAGTCGCTGCTGGCCTTCAAGCGCGCCGGTGCCAACGGCATCCTGACCTAC
TTCGCGCTGGACGCCGCGCGCCTGCTGCGCGGCTGA

Upstream 100 bases:

>100_bases
GCGTCGCGGGGGACACCGCAAGGAAAGGGCCGCTGCGGTGTCCGTGCATAGGACGGACAATCGTCGCAAAAGTTTTCAAC
CTTCACACTGTGTGTCAGCC

Downstream 100 bases:

>100_bases
CCCGGCTCAGGTCGAGGGCTTGGCCTGCGCCGTGCCCAGCGCGCGGCGCAGGCTGGCCAGGAAGGTCTCGGCGCGCTCGA
AGCCGACCACGCGCACCGGC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIASFPDHRPRRMRRDDFSRRMMRESRLTADDLIYPVFILEGTNVRQAVPSMPGVERVSVDVLLGVAEQCVQLGIPVLAL
FPVIEPSLKTPDGIEATNADGLIPRAVKALKARFPELGILTDVALDPYTSHGQDGVLDDAGYVLNEETVEILTRQALVQA
EAGVDIVAPSDMMDGRIGAIRLALENDDHIYTRIMAYAAKYASAFYGPFRDAVGSAANLGKGNKMTYQMDPANSDEALRE
VALDIAEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKVVLESLLAFKRAGANGILTY
FALDAARLLRG

Sequences:

>Translated_331_residues
MIASFPDHRPRRMRRDDFSRRMMRESRLTADDLIYPVFILEGTNVRQAVPSMPGVERVSVDVLLGVAEQCVQLGIPVLAL
FPVIEPSLKTPDGIEATNADGLIPRAVKALKARFPELGILTDVALDPYTSHGQDGVLDDAGYVLNEETVEILTRQALVQA
EAGVDIVAPSDMMDGRIGAIRLALENDDHIYTRIMAYAAKYASAFYGPFRDAVGSAANLGKGNKMTYQMDPANSDEALRE
VALDIAEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKVVLESLLAFKRAGANGILTY
FALDAARLLRG
>Mature_331_residues
MIASFPDHRPRRMRRDDFSRRMMRESRLTADDLIYPVFILEGTNVRQAVPSMPGVERVSVDVLLGVAEQCVQLGIPVLAL
FPVIEPSLKTPDGIEATNADGLIPRAVKALKARFPELGILTDVALDPYTSHGQDGVLDDAGYVLNEETVEILTRQALVQA
EAGVDIVAPSDMMDGRIGAIRLALENDDHIYTRIMAYAAKYASAFYGPFRDAVGSAANLGKGNKMTYQMDPANSDEALRE
VALDIAEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKVVLESLLAFKRAGANGILTY
FALDAARLLRG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=307, Percent_Identity=43.3224755700326, Blast_Score=225, Evalue=4e-59,
Organism=Escherichia coli, GI87081728, Length=325, Percent_Identity=45.8461538461538, Blast_Score=251, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6321398, Length=306, Percent_Identity=40.5228758169935, Blast_Score=203, Evalue=2e-53,
Organism=Drosophila melanogaster, GI21358291, Length=304, Percent_Identity=42.1052631578947, Blast_Score=231, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36384; Mature: 36384

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIASFPDHRPRRMRRDDFSRRMMRESRLTADDLIYPVFILEGTNVRQAVPSMPGVERVSV
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEEEEECCCHHHHCCCCCCCHHHHH
DVLLGVAEQCVQLGIPVLALFPVIEPSLKTPDGIEATNADGLIPRAVKALKARFPELGIL
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
TDVALDPYTSHGQDGVLDDAGYVLNEETVEILTRQALVQAEAGVDIVAPSDMMDGRIGAI
HHCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCEEECCCHHCCCCEEEE
RLALENDDHIYTRIMAYAAKYASAFYGPFRDAVGSAANLGKGNKMTYQMDPANSDEALRE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCCHHHHHHH
VALDIAEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDK
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCHHH
VVLESLLAFKRAGANGILTYFALDAARLLRG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIASFPDHRPRRMRRDDFSRRMMRESRLTADDLIYPVFILEGTNVRQAVPSMPGVERVSV
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEEEEECCCHHHHCCCCCCCHHHHH
DVLLGVAEQCVQLGIPVLALFPVIEPSLKTPDGIEATNADGLIPRAVKALKARFPELGIL
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
TDVALDPYTSHGQDGVLDDAGYVLNEETVEILTRQALVQAEAGVDIVAPSDMMDGRIGAI
HHCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCEEECCCHHCCCCEEEE
RLALENDDHIYTRIMAYAAKYASAFYGPFRDAVGSAANLGKGNKMTYQMDPANSDEALRE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCCHHHHHHH
VALDIAEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDK
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCHHH
VVLESLLAFKRAGANGILTYFALDAARLLRG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]