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The map label for this gene is hprT [H]

Identifier: 17547531

GI number: 17547531

Start: 3026111

End: 3026659

Strand: Direct

Name: hprT [H]

Synonym: RSc2812

Alternate gene names: 17547531

Gene position: 3026111-3026659 (Clockwise)

Preceding gene: 17547530

Following gene: 17547536

Centisome position: 81.43

GC content: 64.85

Gene sequence:

>549_bases
ATGCTGAGCGCAGAACAGGCCCGCGAACTGTGGGCCAACTCCGAGGAAATCGTTAGCGAAGCGGCCGTGCACGCCTCGCT
GGACCGCATGGCCGCCGAGATCACCGAGAAGATCGGCGACACCTTTCCGATGGTGCTCTCCGTGATGGGCGGCGCCTGCG
TCTTCACCGGCATGCTGCTGCCCAAGCTGGCCTTCCCGCTGGAGTTCGACTACATCCACCTGTCCCGCTACAACAACAAG
ACGGTGGGCGGCGAGATGCAATGGCGGGTGGCGCCGCGCGAGTCGGTCAAGGACCGCACCGTGCTGGTGCTCGACGACAT
CCTGGACGAAGGCGAGACCATGGCCGCCATCCGCTCGCGCATTATTGACATGGGTGCGGCCGAATTCTATTCCGCCGTGC
TGTGCGAGAAGACACTCGCCAAGGACAAACCGCTGCACCCGGACTTCTGCGGCTTCCCGGTGCCGGACCGCTACGTATTC
GGCTGCGGCATGGACGCCAAGGGCTACTGGCGCAACCTGCCGGCCATCCGGGCACTGCGCAACAGCTGA

Upstream 100 bases:

>100_bases
CCATGGGCGGCCTCAAGGGCGGCATGAAGGGACTGCTTCCCGGCGGCCGCTGAGCCCGTCCGCGATCCGATTGACCAGAA
ACGTTTCACCGACCGATACG

Downstream 100 bases:

>100_bases
TCCGCTGCGGTCGGCAACAAAAAAGGGCGACATTCTGGTCGCCCTTTTTCATTGGCCGCGTCATTGGCCACACATCGCGC
CGCGCTCACAGCTGATGCAC

Product: hypoxanthine-guanine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MLSAEQARELWANSEEIVSEAAVHASLDRMAAEITEKIGDTFPMVLSVMGGACVFTGMLLPKLAFPLEFDYIHLSRYNNK
TVGGEMQWRVAPRESVKDRTVLVLDDILDEGETMAAIRSRIIDMGAAEFYSAVLCEKTLAKDKPLHPDFCGFPVPDRYVF
GCGMDAKGYWRNLPAIRALRNS

Sequences:

>Translated_182_residues
MLSAEQARELWANSEEIVSEAAVHASLDRMAAEITEKIGDTFPMVLSVMGGACVFTGMLLPKLAFPLEFDYIHLSRYNNK
TVGGEMQWRVAPRESVKDRTVLVLDDILDEGETMAAIRSRIIDMGAAEFYSAVLCEKTLAKDKPLHPDFCGFPVPDRYVF
GCGMDAKGYWRNLPAIRALRNS
>Mature_182_residues
MLSAEQARELWANSEEIVSEAAVHASLDRMAAEITEKIGDTFPMVLSVMGGACVFTGMLLPKLAFPLEFDYIHLSRYNNK
TVGGEMQWRVAPRESVKDRTVLVLDDILDEGETMAAIRSRIIDMGAAEFYSAVLCEKTLAKDKPLHPDFCGFPVPDRYVF
GCGMDAKGYWRNLPAIRALRNS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI9910262, Length=170, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI4504483, Length=159, Percent_Identity=25.7861635220126, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI87081689, Length=165, Percent_Identity=25.4545454545455, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17510817, Length=168, Percent_Identity=27.3809523809524, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20280; Mature: 20280

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAEQARELWANSEEIVSEAAVHASLDRMAAEITEKIGDTFPMVLSVMGGACVFTGMLL
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
PKLAFPLEFDYIHLSRYNNKTVGGEMQWRVAPRESVKDRTVLVLDDILDEGETMAAIRSR
HHHHCCCCCCEEEEECCCCCCCCCCEEEEECCHHCCCCCEEEEEHHHHCCCHHHHHHHHH
IIDMGAAEFYSAVLCEKTLAKDKPLHPDFCGFPVPDRYVFGCGMDAKGYWRNLPAIRALR
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCHHHHHHH
NS
CC
>Mature Secondary Structure
MLSAEQARELWANSEEIVSEAAVHASLDRMAAEITEKIGDTFPMVLSVMGGACVFTGMLL
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
PKLAFPLEFDYIHLSRYNNKTVGGEMQWRVAPRESVKDRTVLVLDDILDEGETMAAIRSR
HHHHCCCCCCEEEEECCCCCCCCCCEEEEECCHHCCCCCEEEEEHHHHCCCHHHHHHHHH
IIDMGAAEFYSAVLCEKTLAKDKPLHPDFCGFPVPDRYVFGCGMDAKGYWRNLPAIRALR
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCHHHHHHH
NS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]