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The map label for this gene is popW [H]

Identifier: 17547494

GI number: 17547494

Start: 2985681

End: 2986823

Strand: Reverse

Name: popW [H]

Synonym: RSc2775

Alternate gene names: 17547494

Gene position: 2986823-2985681 (Counterclockwise)

Preceding gene: 17547495

Following gene: 17547492

Centisome position: 80.37

GC content: 62.9

Gene sequence:

>1143_bases
ATGTCCATCCAGATTGATCGCCCGAACAACCATTTCCAGACGCCCTCGACGTGGAATCACGATGCGGGTTCCAGTATCGA
TACCAGCCAGCTCCAGCGCGCAGTACAACTGCTCGACCAGGTCTTGCAGCAGCTCGAAGCGCGCAAGCTGTTCGGGAACA
TGCTGAACCAGCCGGGCGCCGACAACGCCGGCCAGAACCACGCTGGCGGCCACGGCGGCGGTCATCATGGCGGCTCGAAC
GGGTTCGGTGAAAACGGCCGCTTCGGTTCGCCGCACGCCAATTCGCCTGCGCAGCCGGACCTCGAACTGCCGGCCAACAA
GCCCAACAACGGCAAGCACAACACTTCGGCTTCCACGCCGGACACGCAGACGGCGCCGTCTTCCACTTCGCCCACGACCG
GCACGTCCCCGACGCCCACGTCCACCTCCGCGACCGAGGGCAAGGTGGCCTACGGCGTCAAGCCGCCCGAGCCGACCGGC
GTGGTCGACGTCAGCAAGCCGATCGTCGTCAAGGCTGGCGAGACGTTCGACGGCGGCGGCAAGTACTACCGCCCGACCAA
GGAGATGGGCGACGGTTCGCAGAACGAGCACCAGAAGCCGCTGTTCATTCTGGAGCCCGGCGCGACGCTCAAGAACGTGC
AGTATTCCGGCGGCGACGGCATCCACCTGCTGGGCAGTGCCAAGCTGGACCGCGTCGTGAACCGCCAAGTGGGCGAAGAT
GCCATCACCATCGATGGCGCCAAGAACCGCGCGCATGATGCCAAGATCGCCGGGATCGATCCGGCGTCGATCCCCGGAGG
AACGCCCAAAGTGGAGATCACCAACAGCGCCTTCTATGGCGCCAAGGACAAGCTCGCTCAGATCAACGGCGACGTTGACC
TGCAGGTGAAAGGCATGTATGTCAACGGCGCTGGCAAGGTCTTCCGTACCAACGGTGGTGATACGCAGATCAAGGCGACG
GTCAACGTCCAGGATTCGAATTTCCAGAACGTGTCGGAGGCGGTTTTCCGGACCGATTCCAAGTTCTCGACTGCGTCGTT
CTCGGACGATGTGAAATCGGATGCGCCCTTCGATGGACTGGCTCCCGACAAGAGCCAAGTCACAGGCACCAACAAGGTGA
GCTACAAGGCCTACTCGGGCTGA

Upstream 100 bases:

>100_bases
CGGTTTCGGAGCGAGGTTGAAGGGGCCTGAAAGGTACAACTGTCCAACGCGCCTCACGCGAGGGGCGCGGGCTCCCCCAA
CCTTTCTTGCCGAGACAGTC

Downstream 100 bases:

>100_bases
TGCTGCCGCTGGAGCGGGCCGCGCCGCCGGGTGCGGCCTATTTCAGCCAGCCCTTGCGCCGGAAATACACCAGCGGGATC
GCCGCCGACACGATCATCAG

Product: HARPIN-like protein

Products: NA

Alternate protein names: Pectin lyase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSIQIDRPNNHFQTPSTWNHDAGSSIDTSQLQRAVQLLDQVLQQLEARKLFGNMLNQPGADNAGQNHAGGHGGGHHGGSN
GFGENGRFGSPHANSPAQPDLELPANKPNNGKHNTSASTPDTQTAPSSTSPTTGTSPTPTSTSATEGKVAYGVKPPEPTG
VVDVSKPIVVKAGETFDGGGKYYRPTKEMGDGSQNEHQKPLFILEPGATLKNVQYSGGDGIHLLGSAKLDRVVNRQVGED
AITIDGAKNRAHDAKIAGIDPASIPGGTPKVEITNSAFYGAKDKLAQINGDVDLQVKGMYVNGAGKVFRTNGGDTQIKAT
VNVQDSNFQNVSEAVFRTDSKFSTASFSDDVKSDAPFDGLAPDKSQVTGTNKVSYKAYSG

Sequences:

>Translated_380_residues
MSIQIDRPNNHFQTPSTWNHDAGSSIDTSQLQRAVQLLDQVLQQLEARKLFGNMLNQPGADNAGQNHAGGHGGGHHGGSN
GFGENGRFGSPHANSPAQPDLELPANKPNNGKHNTSASTPDTQTAPSSTSPTTGTSPTPTSTSATEGKVAYGVKPPEPTG
VVDVSKPIVVKAGETFDGGGKYYRPTKEMGDGSQNEHQKPLFILEPGATLKNVQYSGGDGIHLLGSAKLDRVVNRQVGED
AITIDGAKNRAHDAKIAGIDPASIPGGTPKVEITNSAFYGAKDKLAQINGDVDLQVKGMYVNGAGKVFRTNGGDTQIKAT
VNVQDSNFQNVSEAVFRTDSKFSTASFSDDVKSDAPFDGLAPDKSQVTGTNKVSYKAYSG
>Mature_379_residues
SIQIDRPNNHFQTPSTWNHDAGSSIDTSQLQRAVQLLDQVLQQLEARKLFGNMLNQPGADNAGQNHAGGHGGGHHGGSNG
FGENGRFGSPHANSPAQPDLELPANKPNNGKHNTSASTPDTQTAPSSTSPTTGTSPTPTSTSATEGKVAYGVKPPEPTGV
VDVSKPIVVKAGETFDGGGKYYRPTKEMGDGSQNEHQKPLFILEPGATLKNVQYSGGDGIHLLGSAKLDRVVNRQVGEDA
ITIDGAKNRAHDAKIAGIDPASIPGGTPKVEITNSAFYGAKDKLAQINGDVDLQVKGMYVNGAGKVFRTNGGDTQIKATV
NVQDSNFQNVSEAVFRTDSKFSTASFSDDVKSDAPFDGLAPDKSQVTGTNKVSYKAYSG

Specific function: Catalyzes the depolymerization of both polygalacturonate and pectins of methyl esterification degree from 22 to 89%, with an endo mode of action. In contrast to the majority of pectate lyases, displays high activity on highly methylated pectins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide lyase 3 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004898
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF03211 Pectate_lyase [H]

EC number: =4.2.2.2; =4.2.2.10 [H]

Molecular weight: Translated: 39810; Mature: 39679

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQIDRPNNHFQTPSTWNHDAGSSIDTSQLQRAVQLLDQVLQQLEARKLFGNMLNQPGA
CEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DNAGQNHAGGHGGGHHGGSNGFGENGRFGSPHANSPAQPDLELPANKPNNGKHNTSASTP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DTQTAPSSTSPTTGTSPTPTSTSATEGKVAYGVKPPEPTGVVDVSKPIVVKAGETFDGGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEECCCCEEEECCCCCCCCC
KYYRPTKEMGDGSQNEHQKPLFILEPGATLKNVQYSGGDGIHLLGSAKLDRVVNRQVGED
CEECCHHHHCCCCCCCCCCCEEEECCCCCEEEEEECCCCCEEEECCHHHHHHHHHHCCCC
AITIDGAKNRAHDAKIAGIDPASIPGGTPKVEITNSAFYGAKDKLAQINGDVDLQVKGMY
EEEECCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCEEEEEEEEE
VNGAGKVFRTNGGDTQIKATVNVQDSNFQNVSEAVFRTDSKFSTASFSDDVKSDAPFDGL
ECCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
APDKSQVTGTNKVSYKAYSG
CCCHHHCCCCCCEEEEEECC
>Mature Secondary Structure 
SIQIDRPNNHFQTPSTWNHDAGSSIDTSQLQRAVQLLDQVLQQLEARKLFGNMLNQPGA
EEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DNAGQNHAGGHGGGHHGGSNGFGENGRFGSPHANSPAQPDLELPANKPNNGKHNTSASTP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DTQTAPSSTSPTTGTSPTPTSTSATEGKVAYGVKPPEPTGVVDVSKPIVVKAGETFDGGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEECCCCEEEECCCCCCCCC
KYYRPTKEMGDGSQNEHQKPLFILEPGATLKNVQYSGGDGIHLLGSAKLDRVVNRQVGED
CEECCHHHHCCCCCCCCCCCEEEECCCCCEEEEEECCCCCEEEECCHHHHHHHHHHCCCC
AITIDGAKNRAHDAKIAGIDPASIPGGTPKVEITNSAFYGAKDKLAQINGDVDLQVKGMY
EEEECCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCEEEEEEEEE
VNGAGKVFRTNGGDTQIKATVNVQDSNFQNVSEAVFRTDSKFSTASFSDDVKSDAPFDGL
ECCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
APDKSQVTGTNKVSYKAYSG
CCCHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA