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The map label for this gene is btuB [H]

Identifier: 17547119

GI number: 17547119

Start: 2606993

End: 2608858

Strand: Reverse

Name: btuB [H]

Synonym: RSc2400

Alternate gene names: 17547119

Gene position: 2608858-2606993 (Counterclockwise)

Preceding gene: 17547124

Following gene: 17547118

Centisome position: 70.2

GC content: 67.1

Gene sequence:

>1866_bases
ATGGGCGCGCTTGCCGGCGCGCTGGCGGGGGTTGCGGCGGGGCCGGTCTGGGCACAGGGCGACGTGCAGTCGGCCGGCAT
GCCTGTCGGCGAACTCAACCCGACCGTGGTGACGGCGTCGCGCAGCGAGCAGAAGCTTGCCGATGCGCTGCCGCATACCA
CGGTCATCTCGCGCGCCGACATCGAGCGGTCGCAGGCACCGGATGCGGTGTCGCTGCTGCGCCGCGAGGCGGGCATCGAG
ATCGCCCAGGCGGGCGGGCCGGGCGCGGCCGCGAGCCTCTTCATGCGGGGCGCCGGGTCCAACCAGACGCTCATTCTGAT
CGACGGCGTGCGCGTGAGTTCCGGCACCACCGGCACGACGCAGATCGAGCAGCTGATGGCCGATCAGATCGACCATATCG
AGATCGTGCGCGGCAATGTCTCCGCGCTGTACGGCTCGGATGCGATCGGCGGGGTGGTGCAGATCTTCACGCGCAACGGG
CGCGGGCACGCGCCGCTGGCCAACGCGGAAATCGAGTACGGCGCGCGCAATACCAAGCGCGCGCAGGCCGGCATCAGCGG
CCAGCTGGACGCGCGCGGCGACACGTCGTTCGCGCTGTCGGTGTCGGCGTTAAAGACGACGGGTTTCTCGTCGATCAATC
CGAAGCAGGCGCCGAACGCCAACCCGAACGACAACGCGTACGCGAACAAGAGCGTGTCGGCGCAGCTGCAGCATCGCTTC
TCGTCCGACTGGCAGGCGGGGCTGACGTGGTTCCAGACCTGGAGCACCGTGAGCTACGACAACGCGTTCGGCGCGCCCAC
CGACGACAACGAGTCGCACAACCAGGTGCGTGCCATGTCGGCCTACGTGGACGGCAAGCTGACGCCCGACTGGAAGACGC
GCCTGACGCTGTCGCAGGGCGACGACAAGAACCTGAATTTCACCAACGGCCAGGTGCAGGAGCCGGGGCGCTTCAACACG
CGGAACCGCCAGGCGTCGTGGCAGAACGACTGGGCCTTCCTGCCGGACCAGATGCTGAAGGTCGGCTTCGAGCACCTGGA
CCAGACCATCGACACCGATGCCTACTCGCCGCCGACGCGCCGTGTGGACTCGGGCTACATCGGCTATGAAGGCAAGTTCG
GCCGGCACCAGCTGCAGCTGAATCTGCGCCGCGACCGCTACTCCGATTTCGGCGGCGCCAGCAGCTACTATGCCGGCTAC
GGCTTTGCCTTCAACCCGCAGTGGAAGGCGGTGGCCAGCATCAGCAATGCGTTCCGGGCGCCCAGCTTCAACGAGCTGTA
CTACCCGTTCTTCGGCAACCCGAACCTGCAGCCGGAGAAGGCGCGCTCGGTGGAGGGCGGTGTGGAATACCAGAGCACGG
TCGGCCTTGTGCGCATGACGGCATTCGAAACCGACTACAGCAACCTGATCACCAGCGTGTTCGACCCCGTGTCGGGCAAT
TTCCTGGCGGCCAACGTCAACCGCGCGCGGGTGAACGGGCTGGAGACGTCCTGGCGGGGCACGCTCCATGGCGTGGACGT
GCGCGCCAGCTTGACCATCCAGAATCCGCAGGACCTGTCGGCGAACCGGCTGCTGGCGCGCCATGCCCGGCACTTCGGCA
GCGTGTCGGCCTACAAGTCCTTCGGGCCGTTCTCGGCCGGCCTCGAGTGGAATGCCGCCGGCGAGCGGCAGGATTCGGCG
CGTACGCTGGGCGGCTATGGCTTGCTCAACCTGACGGGGCGTTACCAGATCACGCATGATTGGGCGCTGTCGGCGCGCGT
GGAGAACCTGCTCAACAAGAACTACCAGCTGATCGCCCCATACAACACGGCATCGCGCGGGGTGTTCTTCACGCTGTCGT
GGCAACAGCACGCACCGCAACGATGA

Upstream 100 bases:

>100_bases
GCTGCCAGCGACCCGCGGGGGAACGGGAAGGGCTGATGCTATTGGTTTCTTCATCATGAGCTTGACTACAAAGCGGCCGC
GCCGGATGCACCGGGCGGCC

Downstream 100 bases:

>100_bases
CACCCGCATTGCCCGCGCGCGGTTTTGCCTGGCGACTGTGGTCGCTGCTGGCGCTCGCGTGCGTGGCGGTGCTGGTGGCC
TCGCTGATGCTGGGCAGCGT

Product: outer membrane receptor protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MGALAGALAGVAAGPVWAQGDVQSAGMPVGELNPTVVTASRSEQKLADALPHTTVISRADIERSQAPDAVSLLRREAGIE
IAQAGGPGAAASLFMRGAGSNQTLILIDGVRVSSGTTGTTQIEQLMADQIDHIEIVRGNVSALYGSDAIGGVVQIFTRNG
RGHAPLANAEIEYGARNTKRAQAGISGQLDARGDTSFALSVSALKTTGFSSINPKQAPNANPNDNAYANKSVSAQLQHRF
SSDWQAGLTWFQTWSTVSYDNAFGAPTDDNESHNQVRAMSAYVDGKLTPDWKTRLTLSQGDDKNLNFTNGQVQEPGRFNT
RNRQASWQNDWAFLPDQMLKVGFEHLDQTIDTDAYSPPTRRVDSGYIGYEGKFGRHQLQLNLRRDRYSDFGGASSYYAGY
GFAFNPQWKAVASISNAFRAPSFNELYYPFFGNPNLQPEKARSVEGGVEYQSTVGLVRMTAFETDYSNLITSVFDPVSGN
FLAANVNRARVNGLETSWRGTLHGVDVRASLTIQNPQDLSANRLLARHARHFGSVSAYKSFGPFSAGLEWNAAGERQDSA
RTLGGYGLLNLTGRYQITHDWALSARVENLLNKNYQLIAPYNTASRGVFFTLSWQQHAPQR

Sequences:

>Translated_621_residues
MGALAGALAGVAAGPVWAQGDVQSAGMPVGELNPTVVTASRSEQKLADALPHTTVISRADIERSQAPDAVSLLRREAGIE
IAQAGGPGAAASLFMRGAGSNQTLILIDGVRVSSGTTGTTQIEQLMADQIDHIEIVRGNVSALYGSDAIGGVVQIFTRNG
RGHAPLANAEIEYGARNTKRAQAGISGQLDARGDTSFALSVSALKTTGFSSINPKQAPNANPNDNAYANKSVSAQLQHRF
SSDWQAGLTWFQTWSTVSYDNAFGAPTDDNESHNQVRAMSAYVDGKLTPDWKTRLTLSQGDDKNLNFTNGQVQEPGRFNT
RNRQASWQNDWAFLPDQMLKVGFEHLDQTIDTDAYSPPTRRVDSGYIGYEGKFGRHQLQLNLRRDRYSDFGGASSYYAGY
GFAFNPQWKAVASISNAFRAPSFNELYYPFFGNPNLQPEKARSVEGGVEYQSTVGLVRMTAFETDYSNLITSVFDPVSGN
FLAANVNRARVNGLETSWRGTLHGVDVRASLTIQNPQDLSANRLLARHARHFGSVSAYKSFGPFSAGLEWNAAGERQDSA
RTLGGYGLLNLTGRYQITHDWALSARVENLLNKNYQLIAPYNTASRGVFFTLSWQQHAPQR
>Mature_620_residues
GALAGALAGVAAGPVWAQGDVQSAGMPVGELNPTVVTASRSEQKLADALPHTTVISRADIERSQAPDAVSLLRREAGIEI
AQAGGPGAAASLFMRGAGSNQTLILIDGVRVSSGTTGTTQIEQLMADQIDHIEIVRGNVSALYGSDAIGGVVQIFTRNGR
GHAPLANAEIEYGARNTKRAQAGISGQLDARGDTSFALSVSALKTTGFSSINPKQAPNANPNDNAYANKSVSAQLQHRFS
SDWQAGLTWFQTWSTVSYDNAFGAPTDDNESHNQVRAMSAYVDGKLTPDWKTRLTLSQGDDKNLNFTNGQVQEPGRFNTR
NRQASWQNDWAFLPDQMLKVGFEHLDQTIDTDAYSPPTRRVDSGYIGYEGKFGRHQLQLNLRRDRYSDFGGASSYYAGYG
FAFNPQWKAVASISNAFRAPSFNELYYPFFGNPNLQPEKARSVEGGVEYQSTVGLVRMTAFETDYSNLITSVFDPVSGNF
LAANVNRARVNGLETSWRGTLHGVDVRASLTIQNPQDLSANRLLARHARHFGSVSAYKSFGPFSAGLEWNAAGERQDSAR
TLGGYGLLNLTGRYQITHDWALSARVENLLNKNYQLIAPYNTASRGVFFTLSWQQHAPQR

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=607, Percent_Identity=26.8533772652389, Blast_Score=175, Evalue=9e-45,
Organism=Escherichia coli, GI1788478, Length=127, Percent_Identity=39.3700787401575, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67469; Mature: 67338

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGALAGALAGVAAGPVWAQGDVQSAGMPVGELNPTVVTASRSEQKLADALPHTTVISRAD
CCCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEHHH
IERSQAPDAVSLLRREAGIEIAQAGGPGAAASLFMRGAGSNQTLILIDGVRVSSGTTGTT
CCHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHEECCCCCCEEEEEECEEECCCCCCHH
QIEQLMADQIDHIEIVRGNVSALYGSDAIGGVVQIFTRNGRGHAPLANAEIEYGARNTKR
HHHHHHHCCCCEEEEEECCCEEEECCCHHCCEEEEEECCCCCCCCCCCCEEECCCCCCHH
AQAGISGQLDARGDTSFALSVSALKTTGFSSINPKQAPNANPNDNAYANKSVSAQLQHRF
HHCCCCCCCCCCCCCEEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SSDWQAGLTWFQTWSTVSYDNAFGAPTDDNESHNQVRAMSAYVDGKLTPDWKTRLTLSQG
CCCHHHCCHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCCEEEEEECC
DDKNLNFTNGQVQEPGRFNTRNRQASWQNDWAFLPDQMLKVGFEHLDQTIDTDAYSPPTR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
RVDSGYIGYEGKFGRHQLQLNLRRDRYSDFGGASSYYAGYGFAFNPQWKAVASISNAFRA
CCCCCCCCCCCCCCCEEEEEEECHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCC
PSFNELYYPFFGNPNLQPEKARSVEGGVEYQSTVGLVRMTAFETDYSNLITSVFDPVSGN
CCCCCEEEEECCCCCCCHHHHHCCCCCCCHHHHHCEEEEEEECCCHHHHHHHHHCCCCCC
FLAANVNRARVNGLETSWRGTLHGVDVRASLTIQNPQDLSANRLLARHARHFGSVSAYKS
EEEEECCHHEECCCCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHC
FGPFSAGLEWNAAGERQDSARTLGGYGLLNLTGRYQITHDWALSARVENLLNKNYQLIAP
CCCCCCCCEECCCCCCCCCHHHCCCEEEEEECEEEEEEECHHHHHHHHHHHCCCEEEEEC
YNTASRGVFFTLSWQQHAPQR
CCCCCCCEEEEEEHHHCCCCC
>Mature Secondary Structure 
GALAGALAGVAAGPVWAQGDVQSAGMPVGELNPTVVTASRSEQKLADALPHTTVISRAD
CCHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEHHH
IERSQAPDAVSLLRREAGIEIAQAGGPGAAASLFMRGAGSNQTLILIDGVRVSSGTTGTT
CCHHCCCHHHHHHHHHCCCEEEECCCCCHHHHHHEECCCCCCEEEEEECEEECCCCCCHH
QIEQLMADQIDHIEIVRGNVSALYGSDAIGGVVQIFTRNGRGHAPLANAEIEYGARNTKR
HHHHHHHCCCCEEEEEECCCEEEECCCHHCCEEEEEECCCCCCCCCCCCEEECCCCCCHH
AQAGISGQLDARGDTSFALSVSALKTTGFSSINPKQAPNANPNDNAYANKSVSAQLQHRF
HHCCCCCCCCCCCCCEEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SSDWQAGLTWFQTWSTVSYDNAFGAPTDDNESHNQVRAMSAYVDGKLTPDWKTRLTLSQG
CCCHHHCCHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCCEEEEEECC
DDKNLNFTNGQVQEPGRFNTRNRQASWQNDWAFLPDQMLKVGFEHLDQTIDTDAYSPPTR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
RVDSGYIGYEGKFGRHQLQLNLRRDRYSDFGGASSYYAGYGFAFNPQWKAVASISNAFRA
CCCCCCCCCCCCCCCEEEEEEECHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCC
PSFNELYYPFFGNPNLQPEKARSVEGGVEYQSTVGLVRMTAFETDYSNLITSVFDPVSGN
CCCCCEEEEECCCCCCCHHHHHCCCCCCCHHHHHCEEEEEEECCCHHHHHHHHHCCCCCC
FLAANVNRARVNGLETSWRGTLHGVDVRASLTIQNPQDLSANRLLARHARHFGSVSAYKS
EEEEECCHHEECCCCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHC
FGPFSAGLEWNAAGERQDSARTLGGYGLLNLTGRYQITHDWALSARVENLLNKNYQLIAP
CCCCCCCCEECCCCCCCCCHHHCCCEEEEEECEEEEEEECHHHHHHHHHHHCCCEEEEEC
YNTASRGVFFTLSWQQHAPQR
CCCCCCCEEEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA