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The map label for this gene is tISRso14b [C]

Identifier: 17547124

GI number: 17547124

Start: 2611716

End: 2612543

Strand: Reverse

Name: tISRso14b [C]

Synonym: RSc2405

Alternate gene names: 17547124

Gene position: 2612543-2611716 (Counterclockwise)

Preceding gene: 17547125

Following gene: 17547119

Centisome position: 70.3

GC content: 62.8

Gene sequence:

>828_bases
GTGGCGATTCGGCGGAAGGTCAGCATCTCCGAGCGTCGCGCCTGCCGGCTTGTCGGGCTGTCCCGCAGCGTGCTGCATTA
CGAAGCGCAAACTGCGCCAGGAAACGAGATACTCAAAGCGCGGCTGGTTGAGCTGGCGCATGAGCGTCGGCGCTTTGGTT
ACCGGCGACTGCACGTTCTGCTGGAGCGCGAGGGTCTGCACGCGAATCACAAACGCGTACACCGGTTATATCGCGAGGCT
GGCTTGGCTGTGCGGCGTCGGCGGCGACGCCACGGCGTGATGGTCGAGCGCGAGCGACTGGCTCTGCCGGCCGCGCCGAA
CGAGGTCTGGTCGATAGACTTCGTGATGGACGCGTTGTCCAATGGGCGTCGCCTGAAGTGCCTGACCATCGTTGATGACT
TCACGAAAGAAGCGGTCGACATCGTCGTAGACCACGGCATCTCAGGCTTGTATGTGGCACGAGCGCTGGACCATGCGGCG
CGCTTCAGGGGCTACCCCAAAGCGCTGCGCACGGACCAAGGGCCGGAGTTCACGAGCCGAGCGCTGGACCAGTGGGCATA
CGCCAGCGGCGTGTCCCTGAAATTGATTCAGGCTGGCAAACCGACGCAGAATGCCTACATCGAGTCGTTCAACGGCAAGT
TCCGAGACGAATGCCTGAATGAGCACTGGTTCACGAGCCTTGCCCATGCACGCGCTGTCATCGCGGCGTGGCGCCAGGAT
TACAACGAGGCGCGGCCGCACAGCGCCTTGAATTACCAACCGCCTGCAGAATTCGCAGCGCGGTATCGAGCAACAGCGGT
GGCTCCTGCCGCTCAGGAGCAAACTTGA

Upstream 100 bases:

>100_bases
AGCTGAAGAAGCTGCTGGCCGAAGCGATGCTGGACATGGAAGCGCTGAAGGTGGTGGTCAAGGGAAAGCCCTGAGCCCGC
AAGCCAAGCGCGAGGCGGTC

Downstream 100 bases:

>100_bases
ATTGGGCTTTACTAGAAGCCCTCTGGCCCTGTTGCCGGGGGCAGGTCAGGTCGACCAGCTTGGTCTCAGCGGCCGTGCGT
GCCTCGCGCGACAGCGTGAA

Product: ISRSO14-transposase orfB protein

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MAIRRKVSISERRACRLVGLSRSVLHYEAQTAPGNEILKARLVELAHERRRFGYRRLHVLLEREGLHANHKRVHRLYREA
GLAVRRRRRRHGVMVERERLALPAAPNEVWSIDFVMDALSNGRRLKCLTIVDDFTKEAVDIVVDHGISGLYVARALDHAA
RFRGYPKALRTDQGPEFTSRALDQWAYASGVSLKLIQAGKPTQNAYIESFNGKFRDECLNEHWFTSLAHARAVIAAWRQD
YNEARPHSALNYQPPAEFAARYRATAVAPAAQEQT

Sequences:

>Translated_275_residues
MAIRRKVSISERRACRLVGLSRSVLHYEAQTAPGNEILKARLVELAHERRRFGYRRLHVLLEREGLHANHKRVHRLYREA
GLAVRRRRRRHGVMVERERLALPAAPNEVWSIDFVMDALSNGRRLKCLTIVDDFTKEAVDIVVDHGISGLYVARALDHAA
RFRGYPKALRTDQGPEFTSRALDQWAYASGVSLKLIQAGKPTQNAYIESFNGKFRDECLNEHWFTSLAHARAVIAAWRQD
YNEARPHSALNYQPPAEFAARYRATAVAPAAQEQT
>Mature_274_residues
AIRRKVSISERRACRLVGLSRSVLHYEAQTAPGNEILKARLVELAHERRRFGYRRLHVLLEREGLHANHKRVHRLYREAG
LAVRRRRRRHGVMVERERLALPAAPNEVWSIDFVMDALSNGRRLKCLTIVDDFTKEAVDIVVDHGISGLYVARALDHAAR
FRGYPKALRTDQGPEFTSRALDQWAYASGVSLKLIQAGKPTQNAYIESFNGKFRDECLNEHWFTSLAHARAVIAAWRQDY
NEARPHSALNYQPPAEFAARYRATAVAPAAQEQT

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=255, Percent_Identity=27.843137254902, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786462, Length=239, Percent_Identity=25.5230125523013, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31441; Mature: 31310

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRRKVSISERRACRLVGLSRSVLHYEAQTAPGNEILKARLVELAHERRRFGYRRLHVL
CCCCCCCCHHHHHHHHHHCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEREGLHANHKRVHRLYREAGLAVRRRRRRHGVMVERERLALPAAPNEVWSIDFVMDALS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCCHHHHHHHHHHHC
NGRRLKCLTIVDDFTKEAVDIVVDHGISGLYVARALDHAARFRGYPKALRTDQGPEFTSR
CCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHH
ALDQWAYASGVSLKLIQAGKPTQNAYIESFNGKFRDECLNEHWFTSLAHARAVIAAWRQD
HHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNEARPHSALNYQPPAEFAARYRATAVAPAAQEQT
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AIRRKVSISERRACRLVGLSRSVLHYEAQTAPGNEILKARLVELAHERRRFGYRRLHVL
CCCCCCCHHHHHHHHHHCCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEREGLHANHKRVHRLYREAGLAVRRRRRRHGVMVERERLALPAAPNEVWSIDFVMDALS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCCCCHHHHHHHHHHHC
NGRRLKCLTIVDDFTKEAVDIVVDHGISGLYVARALDHAARFRGYPKALRTDQGPEFTSR
CCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHH
ALDQWAYASGVSLKLIQAGKPTQNAYIESFNGKFRDECLNEHWFTSLAHARAVIAAWRQD
HHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNEARPHSALNYQPPAEFAARYRATAVAPAAQEQT
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]