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The map label for this gene is purD

Identifier: 17546910

GI number: 17546910

Start: 2373410

End: 2374678

Strand: Direct

Name: purD

Synonym: RSc2191

Alternate gene names: 17546910

Gene position: 2373410-2374678 (Clockwise)

Preceding gene: 17546909

Following gene: 17546911

Centisome position: 63.86

GC content: 68.64

Gene sequence:

>1269_bases
ATGAAAGTGATGGTTGTCGGTTCGGGCGGACGCGAGCACGCCCTGGCATGGAAGCTGGCACGCTCACCCAAGGTGCAGGT
GGTGTACGTGGCGCCGGGCAACGGCGGCACGGCGCTGGACAAGCGCCTGCAGAACGTGCCGATCACCGATCCGGAAGTGC
TGGCCGCGTTCGCCGAGCGCGAAGGCGTGCACTTCACCGTGGTCGGGCCGGAAGCGCCGCTGGCCGCCGGCATCGTCGAC
CTGTTCCGCGCCAAGGGCCTGCGCATCTTCGGGCCGACCCGCGCCGCCGCGCAGCTCGAATCGTCCAAGGATTTCGCCAA
GGCCTTCATGCAGCGCCACGGTATCCCGACCGCCAAATACCAGACCTTCGGCAACGCGGCCGAGGCACACGCCTACGTGG
ACCGCGAAGGCGCCCCCATCGTCATCAAGGCCGACGGCCTGGCCGCCGGCAAGGGTGTGGTGGTGGCGATGACGCTGGAA
GAAGCGCACGGCGCCATCGACATGATGCTGGCCGACAACCGCCTGGGCGACGCCGGCGCGCGCGTGGTGATCGAAGAATT
CCTGGCCGGCGAGGAAGCCAGCTTCATCGTCGTCTGCGACGGCAAAGACGTGGTGGCCATGGCCACCAGCCAGGACCACA
AGCGCCTGCTGGACGGCGACGCCGGCCCCAACACGGGCGGCATGGGCGCCTACTCGCCCGCGCCGGTGGTCACGCCCACG
CTGCATGCCCGCGTGCTGCGCGAGATCATCCTGCCGACCATCCGCGGCATGGAGAAGGACGGCATCCCCTACACCGGCTT
CCTGTACGCCGGCCTGATGATCGACGCCGACGGCACCCCCAAGACGCTCGAGTTCAACTGCCGCATGGGCGACCCGGAAA
CGCAGCCCATCATGGCGCGCATGAAGACCGACCTGTTCGACGTGCTCGACCGCGCCATCGACGGCAAGCTCGACGGCATG
GAGCTCGACTGGGACCGCCGCACCGCGCTGGGCGTGGTGATGGCGGCCTACAACTATCCCGATACGCCGCGCAAGGGCGA
TGTCATCACCGGTATCCCGAAGGAGACGGAAGACAGCGTCACCTTCCACGCCGGCACCACGCTCAAGGACGGCGCGCTGA
CAACCAGCGGCGGCCGCGTGCTATGCGTGGTCGGCCTGGCCGACACCGTCAAGGCAGCGCAGCGCGCCGCCTACGGCGCA
GTGGAGCAGATCCAGTTCGACGGCGCGCAATACCGCAAGGACATCGGCCACCGCGCCATCCGCCGCTAA

Upstream 100 bases:

>100_bases
GTCATCGGGGAGTAACACCCGAGACGCGCACACAACGGCGATCGGACCCACGATCGCCGTTTGTTTTTTCAGAATTCGAG
TCTCTCAGCAGGTTAAAGCT

Downstream 100 bases:

>100_bases
CGCCACGCAACCTGCCGCGCCGATGCCCCACCGCGGGGCGGCGCGGTCGCCGTCACCCAGGGTGATCCCCCGGCTTGCCC
GGGCGGCGCGCCCGCTACAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKVMVVGSGGREHALAWKLARSPKVQVVYVAPGNGGTALDKRLQNVPITDPEVLAAFAEREGVHFTVVGPEAPLAAGIVD
LFRAKGLRIFGPTRAAAQLESSKDFAKAFMQRHGIPTAKYQTFGNAAEAHAYVDREGAPIVIKADGLAAGKGVVVAMTLE
EAHGAIDMMLADNRLGDAGARVVIEEFLAGEEASFIVVCDGKDVVAMATSQDHKRLLDGDAGPNTGGMGAYSPAPVVTPT
LHARVLREIILPTIRGMEKDGIPYTGFLYAGLMIDADGTPKTLEFNCRMGDPETQPIMARMKTDLFDVLDRAIDGKLDGM
ELDWDRRTALGVVMAAYNYPDTPRKGDVITGIPKETEDSVTFHAGTTLKDGALTTSGGRVLCVVGLADTVKAAQRAAYGA
VEQIQFDGAQYRKDIGHRAIRR

Sequences:

>Translated_422_residues
MKVMVVGSGGREHALAWKLARSPKVQVVYVAPGNGGTALDKRLQNVPITDPEVLAAFAEREGVHFTVVGPEAPLAAGIVD
LFRAKGLRIFGPTRAAAQLESSKDFAKAFMQRHGIPTAKYQTFGNAAEAHAYVDREGAPIVIKADGLAAGKGVVVAMTLE
EAHGAIDMMLADNRLGDAGARVVIEEFLAGEEASFIVVCDGKDVVAMATSQDHKRLLDGDAGPNTGGMGAYSPAPVVTPT
LHARVLREIILPTIRGMEKDGIPYTGFLYAGLMIDADGTPKTLEFNCRMGDPETQPIMARMKTDLFDVLDRAIDGKLDGM
ELDWDRRTALGVVMAAYNYPDTPRKGDVITGIPKETEDSVTFHAGTTLKDGALTTSGGRVLCVVGLADTVKAAQRAAYGA
VEQIQFDGAQYRKDIGHRAIRR
>Mature_422_residues
MKVMVVGSGGREHALAWKLARSPKVQVVYVAPGNGGTALDKRLQNVPITDPEVLAAFAEREGVHFTVVGPEAPLAAGIVD
LFRAKGLRIFGPTRAAAQLESSKDFAKAFMQRHGIPTAKYQTFGNAAEAHAYVDREGAPIVIKADGLAAGKGVVVAMTLE
EAHGAIDMMLADNRLGDAGARVVIEEFLAGEEASFIVVCDGKDVVAMATSQDHKRLLDGDAGPNTGGMGAYSPAPVVTPT
LHARVLREIILPTIRGMEKDGIPYTGFLYAGLMIDADGTPKTLEFNCRMGDPETQPIMARMKTDLFDVLDRAIDGKLDGM
ELDWDRRTALGVVMAAYNYPDTPRKGDVITGIPKETEDSVTFHAGTTLKDGALTTSGGRVLCVVGLADTVKAAQRAAYGA
VEQIQFDGAQYRKDIGHRAIRR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=50.2358490566038, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=50.2358490566038, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=50.2358490566038, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=50.2358490566038, Blast_Score=407, Evalue=1e-113,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=61.7370892018779, Blast_Score=508, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=44.4180522565321, Blast_Score=339, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=42.9213483146067, Blast_Score=335, Evalue=1e-92,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=45.0116009280742, Blast_Score=348, Evalue=5e-96,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=45.0116009280742, Blast_Score=347, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_RALSO (Q8XXC4)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520312.1
- ProteinModelPortal:   Q8XXC4
- SMR:   Q8XXC4
- GeneID:   1221035
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2191
- NMPDR:   fig|267608.1.peg.2191
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   RSOL267608:RSC2191-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 45016; Mature: 45016

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMVVGSGGREHALAWKLARSPKVQVVYVAPGNGGTALDKRLQNVPITDPEVLAAFAER
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHC
EGVHFTVVGPEAPLAAGIVDLFRAKGLRIFGPTRAAAQLESSKDFAKAFMQRHGIPTAKY
CCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHCCHHHHHHHHHHCCCCCHHH
QTFGNAAEAHAYVDREGAPIVIKADGLAAGKGVVVAMTLEEAHGAIDMMLADNRLGDAGA
HHCCCCHHHHEEECCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHH
RVVIEEFLAGEEASFIVVCDGKDVVAMATSQDHKRLLDGDAGPNTGGMGAYSPAPVVTPT
HHHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
LHARVLREIILPTIRGMEKDGIPYTGFLYAGLMIDADGTPKTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHH
MKTDLFDVLDRAIDGKLDGMELDWDRRTALGVVMAAYNYPDTPRKGDVITGIPKETEDSV
HHHHHHHHHHHHHCCCCCCEEECCCHHHHHEEEEEECCCCCCCCCCCEEECCCCCCCCCE
TFHAGTTLKDGALTTSGGRVLCVVGLADTVKAAQRAAYGAVEQIQFDGAQYRKDIGHRAI
EEEECCEECCCCEEECCCEEEEEEECHHHHHHHHHHHHCCHHHEECCCHHHHHHHHHHHH
RR
CC
>Mature Secondary Structure
MKVMVVGSGGREHALAWKLARSPKVQVVYVAPGNGGTALDKRLQNVPITDPEVLAAFAER
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHC
EGVHFTVVGPEAPLAAGIVDLFRAKGLRIFGPTRAAAQLESSKDFAKAFMQRHGIPTAKY
CCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHCCHHHHHHHHHHCCCCCHHH
QTFGNAAEAHAYVDREGAPIVIKADGLAAGKGVVVAMTLEEAHGAIDMMLADNRLGDAGA
HHCCCCHHHHEEECCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHH
RVVIEEFLAGEEASFIVVCDGKDVVAMATSQDHKRLLDGDAGPNTGGMGAYSPAPVVTPT
HHHHHHHHCCCCCCEEEEECCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
LHARVLREIILPTIRGMEKDGIPYTGFLYAGLMIDADGTPKTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEEEECCCCCCCHHHHH
MKTDLFDVLDRAIDGKLDGMELDWDRRTALGVVMAAYNYPDTPRKGDVITGIPKETEDSV
HHHHHHHHHHHHHCCCCCCEEECCCHHHHHEEEEEECCCCCCCCCCCEEECCCCCCCCCE
TFHAGTTLKDGALTTSGGRVLCVVGLADTVKAAQRAAYGAVEQIQFDGAQYRKDIGHRAI
EEEECCEECCCCEEECCCEEEEEEECHHHHHHHHHHHHCCHHHEECCCHHHHHHHHHHHH
RR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852