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The map label for this gene is leuA1

Identifier: 17546791

GI number: 17546791

Start: 2244697

End: 2246238

Strand: Reverse

Name: leuA1

Synonym: RSc2072

Alternate gene names: 17546791

Gene position: 2246238-2244697 (Counterclockwise)

Preceding gene: 17546792

Following gene: 17546789

Centisome position: 60.44

GC content: 65.82

Gene sequence:

>1542_bases
ATGAGCGACAAACTGATTATTTTCGACACCACCTTGCGAGACGGCGAGCAGTCGCCCGGGGCGTCGATGACCAAGGAAGA
AAAGATCCGCATCGCCAAGCAGCTCGAGCGGCTGAAGGTGGACGTGATCGAGGCGGGGTTCGCCGCCAGCTCCAACGGCG
ACTTCGAGGCCATCCGCGCCATCGCCCAGTCGGTCAAGGATTCCCGCATCTGCTCGCTGGCGCGTGCGAACGACCGCGAC
ATCTCCCGCGCCGCCGAGGCGCTCAAGCCGGCAGGGCAGTTCCGCATCCACACCTTCATCGCCACGTCCGCGCTGCATAT
GGAGAAGAAGCTGCGCATGACGCCGGACCAGGTGTACGAGCAGGCCCGCCTGGCTGTGCGTTTCGCGCGTCAGTTCACGG
ACGACATCGAGTTCTCGCCGGAAGACGGCAGCCGCTCCGACATGGATTTCCTGTGCCGCGTGCTGGAAGGCGTGATCGCC
GAGGGGGCCACCACCATCAACCTGCCGGACACGGTCGGCTATGCGGTGCCGGAAGGCTATGCCGCGCTGATCCGCTCGGT
GCGCGAGCGCATTCCCAACTCCGACAAGGCGATCTGGTCGGTGCACTGCCACAATGACCTGGGCATGGCGGTCGCGAACT
CGCTGGCCGCGGTCAAGCTGGGCGGCGCGCGCCAGATCGAGTGCACGATCAACGGCCTGGGCGAGCGCGCCGGTAACACC
AGCCTCGAAGAGGTGGTGATGGCCGTCAAGACCCGCCGCGACTACTTCAACCTCGATGTCGGCGTCGATACCACGCAGAT
CGTGCCGGCCTCCAAGCTGGTCTCGCAGATCACCGGCTTCGTGGTGCAGCCGAACAAGGCGGTGGTGGGCGCCAACGCCT
TCGCGCATGCCTCCGGCATCCACCAGGACGGCGTGCTCAAGGCGCGCGATACCTACGAGATCATGCGCGCCGAAGACGTG
GGCTGGACCGCCAACAAGATCGTGCTGGGCAAGCTCTCGGGCCGCAACGCGTTCAAGCAGCGTCTGCAGGAGCTCGGCAT
CGAGCTCGATTCCGAAGCCGAACTGAACGCCGCCTTCACGCGCTTCAAGGAGCTGGCCGACCAGAAGGCCGAGATCTTCG
ATGAGGACATCGTCGCCATCGTCTCCAACGAAGCGCAGCACGCCGAGGGTGAGCACTTCCGCTTCGTGTCGCTGTCGCAG
CGCTCGGAGACCGGCGAGCGGCCGCACGCGCGCATCGTCTTCGTCGCCGACGGCAAGGAAGTGACGGGCGAGGCAGAGGG
CAACGGCCCGGTCGACGCGACGCTCAACGCCATCGAGAGCAAGGTCGCCAGCGGCGCCGAACAGCTGCTGTACTCGGTCA
ACGCCATTACCACCGGCACCCAGGCGCAGGGCGAAGTGACGGTGCGCCTGTCCAAGTCGGGCCGCATCGTCAACGGCGTG
GGCACCGATCCGGACATCGTCGCGGCATCGGCCAAGGCGTACCTGGCGGCGCTGAACAAGCTGCAGGACAAGAGCAGCGA
GAAGCTCAATCCGCAGATCTGA

Upstream 100 bases:

>100_bases
ATGCACGCCGGGGCGTTTTGCATTCTGCGCCGCGAAAAAAGGGCCGCCCGAGATCACCACCGAGATCGACGTCAAAAACG
TCACCCCACAAGGAGCCGCC

Downstream 100 bases:

>100_bases
CTTCGGGTCTCGGATTTCGATCGACACATGCAGGCGCCAGCCCCGGCCTGAACACGATCACAACAGAGCGCGCACCCGGT
GACCCACCGGGCCGCGAAGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERLKVDVIEAGFAASSNGDFEAIRAIAQSVKDSRICSLARANDRD
ISRAAEALKPAGQFRIHTFIATSALHMEKKLRMTPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIA
EGATTINLPDTVGYAVPEGYAALIRSVRERIPNSDKAIWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
SLEEVVMAVKTRRDYFNLDVGVDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGIELDSEAELNAAFTRFKELADQKAEIFDEDIVAIVSNEAQHAEGEHFRFVSLSQ
RSETGERPHARIVFVADGKEVTGEAEGNGPVDATLNAIESKVASGAEQLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGV
GTDPDIVAASAKAYLAALNKLQDKSSEKLNPQI

Sequences:

>Translated_513_residues
MSDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERLKVDVIEAGFAASSNGDFEAIRAIAQSVKDSRICSLARANDRD
ISRAAEALKPAGQFRIHTFIATSALHMEKKLRMTPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIA
EGATTINLPDTVGYAVPEGYAALIRSVRERIPNSDKAIWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
SLEEVVMAVKTRRDYFNLDVGVDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGIELDSEAELNAAFTRFKELADQKAEIFDEDIVAIVSNEAQHAEGEHFRFVSLSQ
RSETGERPHARIVFVADGKEVTGEAEGNGPVDATLNAIESKVASGAEQLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGV
GTDPDIVAASAKAYLAALNKLQDKSSEKLNPQI
>Mature_512_residues
SDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERLKVDVIEAGFAASSNGDFEAIRAIAQSVKDSRICSLARANDRDI
SRAAEALKPAGQFRIHTFIATSALHMEKKLRMTPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAE
GATTINLPDTVGYAVPEGYAALIRSVRERIPNSDKAIWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNTS
LEEVVMAVKTRRDYFNLDVGVDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVG
WTANKIVLGKLSGRNAFKQRLQELGIELDSEAELNAAFTRFKELADQKAEIFDEDIVAIVSNEAQHAEGEHFRFVSLSQR
SETGERPHARIVFVADGKEVTGEAEGNGPVDATLNAIESKVASGAEQLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGVG
TDPDIVAASAKAYLAALNKLQDKSSEKLNPQI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=502, Percent_Identity=50.199203187251, Blast_Score=464, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6324682, Length=538, Percent_Identity=27.3234200743494, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324225, Length=538, Percent_Identity=27.1375464684015, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6320019, Length=388, Percent_Identity=28.8659793814433, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6320071, Length=388, Percent_Identity=28.8659793814433, Blast_Score=129, Evalue=1e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU11_RALSO (Q8XXP1)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520193.1
- ProteinModelPortal:   Q8XXP1
- SMR:   Q8XXP1
- GeneID:   1220913
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2072
- NMPDR:   fig|267608.1.peg.2072
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   RSOL267608:RSC2072-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55573; Mature: 55442

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERLKVDVIEAGFAASSNGDFEAIRA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAQSVKDSRICSLARANDRDISRAAEALKPAGQFRIHTFIATSALHMEKKLRMTPDQVYE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHH
QARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAEGATTINLPDTVGYAVPEGY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHH
AALIRSVRERIPNSDKAIWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCHHEEEECCCEEEEEEEECCCCCCCCC
SLEEVVMAVKTRRDYFNLDVGVDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCEEEEECCCCCHHCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGIELDSEAELNAAFT
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHH
RFKELADQKAEIFDEDIVAIVSNEAQHAEGEHFRFVSLSQRSETGERPHARIVFVADGKE
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCE
VTGEAEGNGPVDATLNAIESKVASGAEQLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGV
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCEEECC
GTDPDIVAASAKAYLAALNKLQDKSSEKLNPQI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERLKVDVIEAGFAASSNGDFEAIRA
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAQSVKDSRICSLARANDRDISRAAEALKPAGQFRIHTFIATSALHMEKKLRMTPDQVYE
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHH
QARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAEGATTINLPDTVGYAVPEGY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHH
AALIRSVRERIPNSDKAIWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCHHEEEECCCEEEEEEEECCCCCCCCC
SLEEVVMAVKTRRDYFNLDVGVDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCEEEEECCCCCHHCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGIELDSEAELNAAFT
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHH
RFKELADQKAEIFDEDIVAIVSNEAQHAEGEHFRFVSLSQRSETGERPHARIVFVADGKE
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCE
VTGEAEGNGPVDATLNAIESKVASGAEQLLYSVNAITTGTQAQGEVTVRLSKSGRIVNGV
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCEEECC
GTDPDIVAASAKAYLAALNKLQDKSSEKLNPQI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852