Click here to switch to the map view.

The map label for this gene is bigR [H]

Identifier: 17546262

GI number: 17546262

Start: 1649698

End: 1650063

Strand: Reverse

Name: bigR [H]

Synonym: RSc1543

Alternate gene names: 17546262

Gene position: 1650063-1649698 (Counterclockwise)

Preceding gene: 17546264

Following gene: 17546261

Centisome position: 44.4

GC content: 68.03

Gene sequence:

>366_bases
ATGCCCCGATCGACCGCCGTCGCCACCACGGCGCCCCCCGCTCCGCTGCCCGAGCATGCCATCGACATGCTGCGGGATGC
CGCGGCCCAGGCCTGTGCCGTGCTCAAGGCCATGGCCCACGAAGACCGCCTGCTGCTGCTCTGTCAGCTGACCCAGGGCG
AACGCAACGTCGGCGAGCTCGAGATGCTGGTCGGCCTGCACCAGCCCAGCCTGTCGCAGCACCTGGGCGTCCTGCGCGAC
GAAGGCCTGGTGGCCACGCGCCGCGACGGCAAATACATCTATTACCGGCTCGCGAGCTTCGAGGTCATCCAGATCATGCA
GACGCTGTCGTCGCTGTATTGCGGTCAGGCCCTGCCCCCATCTTAG

Upstream 100 bases:

>100_bases
GGCGGGCATGGCAGGACTTCCCCAAACAATCTATATTTTAATAGAATATCGTCAGACATTCTGTCTGCCAATAGAGCACC
TTTCAACAACCGCGCCCGCC

Downstream 100 bases:

>100_bases
AAGGCCGCGCCCATCTGGTCATTTTGTGCTGCGGCATGTGGCGGTTGCCCTGGCACCCGCTGCCACCGCAGCGTTCATCG
AACGGCCGGCCGGACGCCTC

Product: transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 121; Mature: 120

Protein sequence:

>121_residues
MPRSTAVATTAPPAPLPEHAIDMLRDAAAQACAVLKAMAHEDRLLLLCQLTQGERNVGELEMLVGLHQPSLSQHLGVLRD
EGLVATRRDGKYIYYRLASFEVIQIMQTLSSLYCGQALPPS

Sequences:

>Translated_121_residues
MPRSTAVATTAPPAPLPEHAIDMLRDAAAQACAVLKAMAHEDRLLLLCQLTQGERNVGELEMLVGLHQPSLSQHLGVLRD
EGLVATRRDGKYIYYRLASFEVIQIMQTLSSLYCGQALPPS
>Mature_120_residues
PRSTAVATTAPPAPLPEHAIDMLRDAAAQACAVLKAMAHEDRLLLLCQLTQGERNVGELEMLVGLHQPSLSQHLGVLRDE
GLVATRRDGKYIYYRLASFEVIQIMQTLSSLYCGQALPPS

Specific function: Represses an operon that comprises itself, XF_0764, XF_0765, XF_0766 and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=92, Percent_Identity=43.4782608695652, Blast_Score=76, Evalue=6e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 13212; Mature: 13080

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRSTAVATTAPPAPLPEHAIDMLRDAAAQACAVLKAMAHEDRLLLLCQLTQGERNVGEL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
EMLVGLHQPSLSQHLGVLRDEGLVATRRDGKYIYYRLASFEVIQIMQTLSSLYCGQALPP
HHHHHCCCCCHHHHHCCCCCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCC
S
C
>Mature Secondary Structure 
PRSTAVATTAPPAPLPEHAIDMLRDAAAQACAVLKAMAHEDRLLLLCQLTQGERNVGEL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
EMLVGLHQPSLSQHLGVLRDEGLVATRRDGKYIYYRLASFEVIQIMQTLSSLYCGQALPP
HHHHHCCCCCHHHHHCCCCCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]