Click here to switch to the map view.

The map label for this gene is bioF

Identifier: 17546197

GI number: 17546197

Start: 1585318

End: 1586568

Strand: Direct

Name: bioF

Synonym: RSc1478

Alternate gene names: 17546197

Gene position: 1585318-1586568 (Clockwise)

Preceding gene: 17546196

Following gene: 17546198

Centisome position: 42.66

GC content: 74.1

Gene sequence:

>1251_bases
ATGCGATTGCTTGACGACCTGCGGGCCGGCCTGGACGCCATCGACGCCGCGCACCTGCGTCGCGTGCGCCGCACCGCCTA
CAGCCCCACCGACCGCAGCCAGCGCATCGACGTGCCGGGCGGCGAGCCGCGCGAGATCCTCGGCTTCTGCGGCAACGACT
ACCTCGGCCTGGCCGCGCATCCGGCGCTGATGCAGGCGGTGGGCGAGGGCGCCCGCCGGTACGGCTTCGGCAGCGGCGCC
TCGCACCTCGTCAGCGGCCACTCGGTGGCCCATGCGAGGTTGGAAGCGCGCATGGCGGCCCTGCAGGCGCCGCATATTCC
CGATGCCGATGCGCTGTGCTTCTGTACCGGCTACATGGCCAACATGGCGGTGGTCAGCGCGATGGCGCAGGCCGGCGGCA
TCCAGCCGGCGCAGGACTGCACGGTCTTCTCCGACGCGCTGAACCACGCCTCGCTGATCGACGGTGCCCGGCTGTCGCGC
GCGGCGGTCAAGGTCTATCCGCACGTCGACCTGGCCGCGCTGGAGGCGCAGCTGGCCGCCTGCGGCAGCCGCAACAAGCT
GATCGTCACCGACGGCGTGTTCAGCATGGACGGCGACATCGCGCCGCTGCCCGAACTGCTGGCGCTGGCAGAGCGCTTCG
ATGCCTGGCTGATCGTCGACGACGCGCACGGCCTGGGCGTGCTCGGCGCGGACGGGGTGGGCGTGCTGTCGCACTTCGGG
CTGCGCTCCGAGCGCCTGATCTATATCGGCACCTTCGGCAAGGCGGCCGGCGGCGCCGGGGCCTGCGTGGTGGCGCACCG
GATGGCGGTCGACTGGCTGGTGCAGCGGGCCCGCACATATATCTTCACGACCGCCACGCCGCCCGCCGTCGCCTGCGGGG
TGGAGGCGGCGCTGGACCTGATTGCGGGGGAGGAGGGCGCTGCGCGCCGGGCCCGGCTGGCCGGCCACATCGCCCGCTGG
AGCGATCACGCGCAGCGGCTGGCGGCACGGTTCGGCTGGCAGTGGATGCCGTCGCCCACCGCGATCCAGCCGCTGGTGAT
CGGCGAGAACGCGCCGGCGCTGGCGCTGGCGGCGGCGCTGGAGCGCGAGGGCATCCGCGTCGCCGCGATCCGCCCGCCGA
CCGTGCCGGCCGGGACCGCGCGGCTGCGCATCACCCTGTCCGCCGCGCACACCGAGGACGACATCGAGCGGCTGGCCCGC
GCGCTGGAGGCGGCCGGCCAATCGCAGCAATCCGCCGCCCGCGCTGCCTGA

Upstream 100 bases:

>100_bases
GTGCTGACCGACGACGAAACCGACACCCTGGTCGAACGCACGCTGCACACCCTCGAAGACACGCTTGCGCAAGCCACCGG
CGCGCACGAAGGAGAAACCC

Downstream 100 bases:

>100_bases
CATGCCGGTGCGCTTCGACTGCTTCGTCACCGGGACCGACACCGAGATCGGCAAGACGCTGGCCAGCGCGGCACTGCTGA
CGGCGCTGGCCGGCGCCGGC

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MRLLDDLRAGLDAIDAAHLRRVRRTAYSPTDRSQRIDVPGGEPREILGFCGNDYLGLAAHPALMQAVGEGARRYGFGSGA
SHLVSGHSVAHARLEARMAALQAPHIPDADALCFCTGYMANMAVVSAMAQAGGIQPAQDCTVFSDALNHASLIDGARLSR
AAVKVYPHVDLAALEAQLAACGSRNKLIVTDGVFSMDGDIAPLPELLALAERFDAWLIVDDAHGLGVLGADGVGVLSHFG
LRSERLIYIGTFGKAAGGAGACVVAHRMAVDWLVQRARTYIFTTATPPAVACGVEAALDLIAGEEGAARRARLAGHIARW
SDHAQRLAARFGWQWMPSPTAIQPLVIGENAPALALAAALEREGIRVAAIRPPTVPAGTARLRITLSAAHTEDDIERLAR
ALEAAGQSQQSAARAA

Sequences:

>Translated_416_residues
MRLLDDLRAGLDAIDAAHLRRVRRTAYSPTDRSQRIDVPGGEPREILGFCGNDYLGLAAHPALMQAVGEGARRYGFGSGA
SHLVSGHSVAHARLEARMAALQAPHIPDADALCFCTGYMANMAVVSAMAQAGGIQPAQDCTVFSDALNHASLIDGARLSR
AAVKVYPHVDLAALEAQLAACGSRNKLIVTDGVFSMDGDIAPLPELLALAERFDAWLIVDDAHGLGVLGADGVGVLSHFG
LRSERLIYIGTFGKAAGGAGACVVAHRMAVDWLVQRARTYIFTTATPPAVACGVEAALDLIAGEEGAARRARLAGHIARW
SDHAQRLAARFGWQWMPSPTAIQPLVIGENAPALALAAALEREGIRVAAIRPPTVPAGTARLRITLSAAHTEDDIERLAR
ALEAAGQSQQSAARAA
>Mature_416_residues
MRLLDDLRAGLDAIDAAHLRRVRRTAYSPTDRSQRIDVPGGEPREILGFCGNDYLGLAAHPALMQAVGEGARRYGFGSGA
SHLVSGHSVAHARLEARMAALQAPHIPDADALCFCTGYMANMAVVSAMAQAGGIQPAQDCTVFSDALNHASLIDGARLSR
AAVKVYPHVDLAALEAQLAACGSRNKLIVTDGVFSMDGDIAPLPELLALAERFDAWLIVDDAHGLGVLGADGVGVLSHFG
LRSERLIYIGTFGKAAGGAGACVVAHRMAVDWLVQRARTYIFTTATPPAVACGVEAALDLIAGEEGAARRARLAGHIARW
SDHAQRLAARFGWQWMPSPTAIQPLVIGENAPALALAAALEREGIRVAAIRPPTVPAGTARLRITLSAAHTEDDIERLAR
ALEAAGQSQQSAARAA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=404, Percent_Identity=36.1386138613861, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI284448556, Length=364, Percent_Identity=37.0879120879121, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI83977442, Length=380, Percent_Identity=36.0526315789474, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI83977440, Length=380, Percent_Identity=36.0526315789474, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI83977444, Length=380, Percent_Identity=36.0526315789474, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI4758668, Length=426, Percent_Identity=25.5868544600939, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI119220554, Length=380, Percent_Identity=27.3684210526316, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4502025, Length=350, Percent_Identity=31.7142857142857, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI40316939, Length=350, Percent_Identity=31.7142857142857, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI5454084, Length=394, Percent_Identity=23.0964467005076, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1786993, Length=365, Percent_Identity=42.7397260273973, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790046, Length=380, Percent_Identity=35.5263157894737, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71994529, Length=372, Percent_Identity=32.7956989247312, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI32566772, Length=380, Percent_Identity=27.1052631578947, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560914, Length=385, Percent_Identity=26.7532467532467, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17560912, Length=373, Percent_Identity=27.3458445040214, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71982625, Length=274, Percent_Identity=23.3576642335766, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71982617, Length=281, Percent_Identity=22.7758007117438, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320438, Length=393, Percent_Identity=31.2977099236641, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6320267, Length=378, Percent_Identity=28.8359788359788, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323954, Length=279, Percent_Identity=26.1648745519713, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24662918, Length=380, Percent_Identity=33.6842105263158, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI17137420, Length=376, Percent_Identity=33.5106382978723, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI17136286, Length=390, Percent_Identity=28.974358974359, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24653280, Length=283, Percent_Identity=25.7950530035336, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653276, Length=283, Percent_Identity=25.7950530035336, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653278, Length=283, Percent_Identity=25.7950530035336, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_RALSO (Q8XZC3)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519599.1
- HSSP:   P12998
- ProteinModelPortal:   Q8XZC3
- GeneID:   1220307
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1478
- NMPDR:   fig|267608.1.peg.1478
- HOGENOM:   HBG649839
- OMA:   KDYASND
- ProtClustDB:   PRK05958
- BioCyc:   RSOL267608:RSC1478-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 43677; Mature: 43677

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 21-21 BINDING 149-149 BINDING 195-195 BINDING 374-374

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLDDLRAGLDAIDAAHLRRVRRTAYSPTDRSQRIDVPGGEPREILGFCGNDYLGLAAH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHCCCCCCCHHCC
PALMQAVGEGARRYGFGSGASHLVSGHSVAHARLEARMAALQAPHIPDADALCFCTGYMA
HHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
NMAVVSAMAQAGGIQPAQDCTVFSDALNHASLIDGARLSRAAVKVYPHVDLAALEAQLAA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHEEEEECCCHHHHHHHHHHH
CGSRNKLIVTDGVFSMDGDIAPLPELLALAERFDAWLIVDDAHGLGVLGADGVGVLSHFG
CCCCCEEEEECCCEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEECCCCHHHHHHCC
LRSERLIYIGTFGKAAGGAGACVVAHRMAVDWLVQRARTYIFTTATPPAVACGVEAALDL
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHH
IAGEEGAARRARLAGHIARWSDHAQRLAARFGWQWMPSPTAIQPLVIGENAPALALAAAL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHH
EREGIRVAAIRPPTVPAGTARLRITLSAAHTEDDIERLARALEAAGQSQQSAARAA
HHCCCEEEEECCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MRLLDDLRAGLDAIDAAHLRRVRRTAYSPTDRSQRIDVPGGEPREILGFCGNDYLGLAAH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHCCCCCCCHHCC
PALMQAVGEGARRYGFGSGASHLVSGHSVAHARLEARMAALQAPHIPDADALCFCTGYMA
HHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
NMAVVSAMAQAGGIQPAQDCTVFSDALNHASLIDGARLSRAAVKVYPHVDLAALEAQLAA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHEEEEECCCHHHHHHHHHHH
CGSRNKLIVTDGVFSMDGDIAPLPELLALAERFDAWLIVDDAHGLGVLGADGVGVLSHFG
CCCCCEEEEECCCEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEECCCCHHHHHHCC
LRSERLIYIGTFGKAAGGAGACVVAHRMAVDWLVQRARTYIFTTATPPAVACGVEAALDL
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHH
IAGEEGAARRARLAGHIARWSDHAQRLAARFGWQWMPSPTAIQPLVIGENAPALALAAAL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHH
EREGIRVAAIRPPTVPAGTARLRITLSAAHTEDDIERLARALEAAGQSQQSAARAA
HHCCCEEEEECCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852