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The map label for this gene is ligA

Identifier: 17546117

GI number: 17546117

Start: 1493654

End: 1496095

Strand: Direct

Name: ligA

Synonym: RSc1398

Alternate gene names: 17546117

Gene position: 1493654-1496095 (Clockwise)

Preceding gene: 17546116

Following gene: 17546118

Centisome position: 40.19

GC content: 70.88

Gene sequence:

>2442_bases
ATGAGCAAGACCGAACATCCCGCGTCCGGCGCGTCGCCCGAGACGCGGGCAGCCGCGCTGCGCGCGACGCTGAACCGCTA
CGCCCACGAGTACTACGTGCTGGACCAGCCCAGCGTGCCGGACGCCGAATACGACCGGCTCTACCGCGAGCTGGAAGCCC
TGGAAGCCGAACACCCAGAACTGCGGACGCCGGATTCGCCCACGCTGCGGGTCGGCGGCGCCGTGCTGCCGGAGTTCGCG
CCGGTGCGGCACGTGGTGCCGATGCTCTCGATCCGCACCGAGACCGACACCACCGCCGGCGGCGCGCTGGACTTCGATGC
CAGCGTGCGCCGCGAACTGGGGCTGGCCGAATCCGATCCGCCCGTCGAATACGCGGCGGAGCTCAAGTTCGATGGCCTGG
CGATCAACCTGCGCTACGAGAAAGGCTTCCTGGTCCAGGCCGCCACGCGCGGCGACGGGGCCACCGGCGAAGACGTTACG
CAGAACATCCGCACCATCCGCCAGATCCCCCTCGGCCTGCGGCCCGTCGGCGGCGCCGTGCCCGACGTGCTCGAAGTGCG
CGGCGAGGTCTACATGCGGCGCGACGATTTCGAGCGCCTCAATGCCCGCCAGCGCGAGCGCGGCGACAAGACCTTCGTCA
ACCCGCGCAACACCGCTGCCGGCGCGGTGCGCCAGCTCGACCCGAAGATGGCCGCCGAGCGCCCGCTGTCGTTCTTCGCC
TATGGCCTGGGCGAGGCGGCCGGCTGGAGCGGGATGCCCGACACGCATTCCGGCATGCTCGATGCACTGGTCGCCTACGG
TTTCCCGGTCAGCAAGGAACGTGCGGCAGTGAAGGGCGGGGAAGGGCTGGTGCAATTCCACGCCGCCATCGGCGCCAAGC
GCGACAGCCTGCCGTTCGATATCGATGGCGTGGTCTACAAGGTCAACTCGCTGGCACTGCAGCGCGAGCTGGGCTTTCGC
ACGCGCGAGCCGCGCTGGGCGGTGGCGCACAAGTACCCGGCGCAGGAGGCGCTGACCACGGTCGAGTCGATCGGCGTGCA
GGTCGGCCGCACCGGGGCCATCACGCCGGTGGCGCGTCTGGTGCCGGTGTTCGTCGGCGGCGTGACGGTCACCAACGCGA
CCCTGCACAACGAAGACGAAGTCCGCCGCAAGGACGTGCGCGTGGGCGACACCGTGATCGTGCGCCGCGCCGGCGACGTG
ATCCCCGAGGTGGTGGCCGTGGTGCTGGAGCGCCGCCCGATGGAAGACGTGCCGGGCAGCGATCTGTTCAACCCCACGCA
GCAGCCCAAGCATCCGCCGTTCGAACTGCCCAGGAGTTGCCCGGTCTGCGGCTCGCACGTGGTGCGCGAAGAGGGCGAGG
CGGTGGCGCGCTGCTCGGGCGGCCTGTTCTGCTCGGCGCAGCGCAAGGAGGCGATCCGCCATTTCGCCGGCCGCCGCATG
ATGGACATCGAAGGCCTGGGCGAGCGCTACATCGACAACCTCGTCGAGCTGGAATACGTGCACGGCATCGCCGACCTGTA
CCGGCTCACGCTCGACGATTTCCTCGAAATGAAGCGCCGCGCCGACGAGCGCGACGGCGTGACCCCGGAGACCGTGGCCG
CCGGCAAGATCGCCACCAAGTGGGCCGAGAACCTGCTGGACGGCATCCGGGCCAGCAAGACCCCGCCGCTGGCGCGCTTC
CTGTTCGCGATGGGCATCCGCCATGTCGGCGAATCGACGGCCAAGACGCTGGCCGACTGGCTGGGCAGCCTGGCGATAGT
CCGCCGCGCGCCGGCGCCGCTGCTGCTGACCTTGCCCGACGTGGGGGCGACCGTCGCCGAGGCCATCGCCGATTTCTTTG
CCGAGCCGAAGAACCAGCAGGCGCTCGACGCCCTGCTCACCGCCGGCGTGGCGCCCCAGGGCGAGCACCCGCCCAGCGCG
AAGCTGCGCGACCAACTGGAGCCCGCCGAACTGTACGCCGCGCTCGGCGTGCCCAAGCTGACGGCCATCCGCAGCAAGCA
GCTGGCGACGCTGGTGCCGTCGCTCGCGCAACTGGCCAACGTCGACACCGCGCAGCTGGAAGGCCTGCCGGCGGACGTGG
CGGCGTCGCTGCTGGCCTGGCTGGATGCCGACGACCATCGCACCCGGCTGGGGACGCTGGACGCGCTGCGCGCCGAACTG
CTTGCCGCCATGCCGGCCGGGGCCGCCGAGGAAGGCGCGCTGAGCGGCAAGACCGTGGTGCTGACCGGCACGCTGCCCAC
CCTGAGCCGCGACGAGGCCAAGGCCATGCTGGAGGCGGCCGGCGCCAAGGTGTCCGGCTCGGTGTCGAAGAAGACCGACT
ACGTTGTGGCTGGCGTTGAGGCGGGCAGCAAGCTCGCCCGCGCGCAGGAGCTGGGCGTGCGGGTTCTGGACGAAGCCGGT
ATGCTCGCGCTGTTGCAGAACCCGCCGGGAGATTCCGCGTGA

Upstream 100 bases:

>100_bases
CAGAAGCATCTGCGCGTGCTGTACGACAAGCTCGAGGCGCGCGGCCTCCCGGCGGGTTCCAGCGCGGCAGTGCGCCTGTT
CAGCCTCTGACCGATCACCG

Downstream 100 bases:

>100_bases
TCCGACCGATCCTGAAGATGGGCGACAGCCGCCTGCTGCGCGTGGCCAAGCCGGTCCAGCGCTTCCAGACGCCGGAGCTG
ACCGCGCTGATCGAAGACAT

Product: DNA ligase (polydeoxyribonucleotide synthase [NAD+]) protein

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 813; Mature: 812

Protein sequence:

>813_residues
MSKTEHPASGASPETRAAALRATLNRYAHEYYVLDQPSVPDAEYDRLYRELEALEAEHPELRTPDSPTLRVGGAVLPEFA
PVRHVVPMLSIRTETDTTAGGALDFDASVRRELGLAESDPPVEYAAELKFDGLAINLRYEKGFLVQAATRGDGATGEDVT
QNIRTIRQIPLGLRPVGGAVPDVLEVRGEVYMRRDDFERLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPLSFFA
YGLGEAAGWSGMPDTHSGMLDALVAYGFPVSKERAAVKGGEGLVQFHAAIGAKRDSLPFDIDGVVYKVNSLALQRELGFR
TREPRWAVAHKYPAQEALTTVESIGVQVGRTGAITPVARLVPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRRAGDV
IPEVVAVVLERRPMEDVPGSDLFNPTQQPKHPPFELPRSCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFAGRRM
MDIEGLGERYIDNLVELEYVHGIADLYRLTLDDFLEMKRRADERDGVTPETVAAGKIATKWAENLLDGIRASKTPPLARF
LFAMGIRHVGESTAKTLADWLGSLAIVRRAPAPLLLTLPDVGATVAEAIADFFAEPKNQQALDALLTAGVAPQGEHPPSA
KLRDQLEPAELYAALGVPKLTAIRSKQLATLVPSLAQLANVDTAQLEGLPADVAASLLAWLDADDHRTRLGTLDALRAEL
LAAMPAGAAEEGALSGKTVVLTGTLPTLSRDEAKAMLEAAGAKVSGSVSKKTDYVVAGVEAGSKLARAQELGVRVLDEAG
MLALLQNPPGDSA

Sequences:

>Translated_813_residues
MSKTEHPASGASPETRAAALRATLNRYAHEYYVLDQPSVPDAEYDRLYRELEALEAEHPELRTPDSPTLRVGGAVLPEFA
PVRHVVPMLSIRTETDTTAGGALDFDASVRRELGLAESDPPVEYAAELKFDGLAINLRYEKGFLVQAATRGDGATGEDVT
QNIRTIRQIPLGLRPVGGAVPDVLEVRGEVYMRRDDFERLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPLSFFA
YGLGEAAGWSGMPDTHSGMLDALVAYGFPVSKERAAVKGGEGLVQFHAAIGAKRDSLPFDIDGVVYKVNSLALQRELGFR
TREPRWAVAHKYPAQEALTTVESIGVQVGRTGAITPVARLVPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRRAGDV
IPEVVAVVLERRPMEDVPGSDLFNPTQQPKHPPFELPRSCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFAGRRM
MDIEGLGERYIDNLVELEYVHGIADLYRLTLDDFLEMKRRADERDGVTPETVAAGKIATKWAENLLDGIRASKTPPLARF
LFAMGIRHVGESTAKTLADWLGSLAIVRRAPAPLLLTLPDVGATVAEAIADFFAEPKNQQALDALLTAGVAPQGEHPPSA
KLRDQLEPAELYAALGVPKLTAIRSKQLATLVPSLAQLANVDTAQLEGLPADVAASLLAWLDADDHRTRLGTLDALRAEL
LAAMPAGAAEEGALSGKTVVLTGTLPTLSRDEAKAMLEAAGAKVSGSVSKKTDYVVAGVEAGSKLARAQELGVRVLDEAG
MLALLQNPPGDSA
>Mature_812_residues
SKTEHPASGASPETRAAALRATLNRYAHEYYVLDQPSVPDAEYDRLYRELEALEAEHPELRTPDSPTLRVGGAVLPEFAP
VRHVVPMLSIRTETDTTAGGALDFDASVRRELGLAESDPPVEYAAELKFDGLAINLRYEKGFLVQAATRGDGATGEDVTQ
NIRTIRQIPLGLRPVGGAVPDVLEVRGEVYMRRDDFERLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPLSFFAY
GLGEAAGWSGMPDTHSGMLDALVAYGFPVSKERAAVKGGEGLVQFHAAIGAKRDSLPFDIDGVVYKVNSLALQRELGFRT
REPRWAVAHKYPAQEALTTVESIGVQVGRTGAITPVARLVPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRRAGDVI
PEVVAVVLERRPMEDVPGSDLFNPTQQPKHPPFELPRSCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFAGRRMM
DIEGLGERYIDNLVELEYVHGIADLYRLTLDDFLEMKRRADERDGVTPETVAAGKIATKWAENLLDGIRASKTPPLARFL
FAMGIRHVGESTAKTLADWLGSLAIVRRAPAPLLLTLPDVGATVAEAIADFFAEPKNQQALDALLTAGVAPQGEHPPSAK
LRDQLEPAELYAALGVPKLTAIRSKQLATLVPSLAQLANVDTAQLEGLPADVAASLLAWLDADDHRTRLGTLDALRAELL
AAMPAGAAEEGALSGKTVVLTGTLPTLSRDEAKAMLEAAGAKVSGSVSKKTDYVVAGVEAGSKLARAQELGVRVLDEAGM
LALLQNPPGDSA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=88, Percent_Identity=46.5909090909091, Blast_Score=80, Evalue=1e-14,
Organism=Escherichia coli, GI1788750, Length=795, Percent_Identity=43.7735849056604, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI87082305, Length=567, Percent_Identity=23.4567901234568, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_RALSO (Q8XZJ7)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519519.1
- HSSP:   O87703
- ProteinModelPortal:   Q8XZJ7
- GeneID:   1220222
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1398
- NMPDR:   fig|267608.1.peg.1398
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK337493
- BioCyc:   RSOL267608:RSC1398-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 87471; Mature: 87340

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 129-129 BINDING 127-127 BINDING 150-150 BINDING 189-189 BINDING 307-307 BINDING 331-331

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTEHPASGASPETRAAALRATLNRYAHEYYVLDQPSVPDAEYDRLYRELEALEAEHPE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
LRTPDSPTLRVGGAVLPEFAPVRHVVPMLSIRTETDTTAGGALDFDASVRRELGLAESDP
CCCCCCCCEEECCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHCCCCCCCC
PVEYAAELKFDGLAINLRYEKGFLVQAATRGDGATGEDVTQNIRTIRQIPLGLRPVGGAV
CHHHHHHEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PDVLEVRGEVYMRRDDFERLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPLSFFA
HHHHHHCCHHEEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHCCHHHHH
YGLGEAAGWSGMPDTHSGMLDALVAYGFPVSKERAAVKGGEGLVQFHAAIGAKRDSLPFD
HHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCC
IDGVVYKVNSLALQRELGFRTREPRWAVAHKYPAQEALTTVESIGVQVGRTGAITPVARL
CCHHEEEHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHCHHCCCCCCCHHHHHH
VPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRRAGDVIPEVVAVVLERRPMEDVPGS
HHHHHCCEEEEECEECCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCC
DLFNPTQQPKHPPFELPRSCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFAGRRM
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCEEECHHHHHHHHHHHCCCE
MDIEGLGERYIDNLVELEYVHGIADLYRLTLDDFLEMKRRADERDGVTPETVAAGKIATK
ECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHH
WAENLLDGIRASKTPPLARFLFAMGIRHVGESTAKTLADWLGSLAIVRRAPAPLLLTLPD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
VGATVAEAIADFFAEPKNQQALDALLTAGVAPQGEHPPSAKLRDQLEPAELYAALGVPKL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCHH
TAIRSKQLATLVPSLAQLANVDTAQLEGLPADVAASLLAWLDADDHRTRLGTLDALRAEL
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LAAMPAGAAEEGALSGKTVVLTGTLPTLSRDEAKAMLEAAGAKVSGSVSKKTDYVVAGVE
HHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
AGSKLARAQELGVRVLDEAGMLALLQNPPGDSA
CCHHHHHHHHHCHHEECCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SKTEHPASGASPETRAAALRATLNRYAHEYYVLDQPSVPDAEYDRLYRELEALEAEHPE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
LRTPDSPTLRVGGAVLPEFAPVRHVVPMLSIRTETDTTAGGALDFDASVRRELGLAESDP
CCCCCCCCEEECCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHCCCCCCCC
PVEYAAELKFDGLAINLRYEKGFLVQAATRGDGATGEDVTQNIRTIRQIPLGLRPVGGAV
CHHHHHHEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
PDVLEVRGEVYMRRDDFERLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPLSFFA
HHHHHHCCHHEEECCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHCCHHHHH
YGLGEAAGWSGMPDTHSGMLDALVAYGFPVSKERAAVKGGEGLVQFHAAIGAKRDSLPFD
HHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCC
IDGVVYKVNSLALQRELGFRTREPRWAVAHKYPAQEALTTVESIGVQVGRTGAITPVARL
CCHHEEEHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHCHHCCCCCCCHHHHHH
VPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRRAGDVIPEVVAVVLERRPMEDVPGS
HHHHHCCEEEEECEECCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCC
DLFNPTQQPKHPPFELPRSCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFAGRRM
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCEEECHHHHHHHHHHHCCCE
MDIEGLGERYIDNLVELEYVHGIADLYRLTLDDFLEMKRRADERDGVTPETVAAGKIATK
ECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHH
WAENLLDGIRASKTPPLARFLFAMGIRHVGESTAKTLADWLGSLAIVRRAPAPLLLTLPD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
VGATVAEAIADFFAEPKNQQALDALLTAGVAPQGEHPPSAKLRDQLEPAELYAALGVPKL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCHH
TAIRSKQLATLVPSLAQLANVDTAQLEGLPADVAASLLAWLDADDHRTRLGTLDALRAEL
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LAAMPAGAAEEGALSGKTVVLTGTLPTLSRDEAKAMLEAAGAKVSGSVSKKTDYVVAGVE
HHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
AGSKLARAQELGVRVLDEAGMLALLQNPPGDSA
CCHHHHHHHHHCHHEECCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852