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The map label for this gene is def

Identifier: 17546118

GI number: 17546118

Start: 1496092

End: 1496625

Strand: Direct

Name: def

Synonym: RSc1399

Alternate gene names: 17546118

Gene position: 1496092-1496625 (Clockwise)

Preceding gene: 17546117

Following gene: 17546119

Centisome position: 40.26

GC content: 67.6

Gene sequence:

>534_bases
GTGATCCGACCGATCCTGAAGATGGGCGACAGCCGCCTGCTGCGCGTGGCCAAGCCGGTCCAGCGCTTCCAGACGCCGGA
GCTGACCGCGCTGATCGAAGACATGTTCGACACCATGGACGCCGCGCGCGGCGCCGGCCTGGCGGCACCGCAGATCGGCG
TCGACCTGCAGGTGGTCATCTTCGGCTTCGACCGCAACGATCGCTATCCGGATGCGCCCGCCGTGCCCAAGACGGTGCTG
ATCAATCCGACCATCGAGCCGCTGTCCGACGCGATGGAAGATGGCTGGGAGGGCTGCCTGTCGGTGCCCGGCCTGCGCGG
CGTGGTGCCGCGCTACACGCGCCTGCGCTATACCGGCTACGACCAGCACGGCCACGCCATCGACCGCATCGCCGAGGGCT
TCCATGCCCGCGTGGTGCAGCACGAGTGCGATCACCTGCAGGGCATCCTGTACCCGATGCGCGTGCAGGATTTCACCCGC
TTCGGCTTCACCGAGATCCTCTTCCCCGAATTGCCCGCCCACCACAACGACTGA

Upstream 100 bases:

>100_bases
AGGCGGGCAGCAAGCTCGCCCGCGCGCAGGAGCTGGGCGTGCGGGTTCTGGACGAAGCCGGTATGCTCGCGCTGTTGCAG
AACCCGCCGGGAGATTCCGC

Downstream 100 bases:

>100_bases
GCGCGGCAGCGGGGTGCCTCACCCGCGTGGCCGGGTGGGGCACCTGTCCACCACGGAGGCGATATGGCGCACGATGTACC
GAGCGGACTGCGCCGACTGG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIRPILKMGDSRLLRVAKPVQRFQTPELTALIEDMFDTMDAARGAGLAAPQIGVDLQVVIFGFDRNDRYPDAPAVPKTVL
INPTIEPLSDAMEDGWEGCLSVPGLRGVVPRYTRLRYTGYDQHGHAIDRIAEGFHARVVQHECDHLQGILYPMRVQDFTR
FGFTEILFPELPAHHND

Sequences:

>Translated_177_residues
MIRPILKMGDSRLLRVAKPVQRFQTPELTALIEDMFDTMDAARGAGLAAPQIGVDLQVVIFGFDRNDRYPDAPAVPKTVL
INPTIEPLSDAMEDGWEGCLSVPGLRGVVPRYTRLRYTGYDQHGHAIDRIAEGFHARVVQHECDHLQGILYPMRVQDFTR
FGFTEILFPELPAHHND
>Mature_177_residues
MIRPILKMGDSRLLRVAKPVQRFQTPELTALIEDMFDTMDAARGAGLAAPQIGVDLQVVIFGFDRNDRYPDAPAVPKTVL
INPTIEPLSDAMEDGWEGCLSVPGLRGVVPRYTRLRYTGYDQHGHAIDRIAEGFHARVVQHECDHLQGILYPMRVQDFTR
FGFTEILFPELPAHHND

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=33.1428571428571, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1789682, Length=148, Percent_Identity=37.1621621621622, Blast_Score=92, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=34.6153846153846, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24645728, Length=182, Percent_Identity=30.2197802197802, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF2_RALSO (Q8XZJ6)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519520.1
- ProteinModelPortal:   Q8XZJ6
- SMR:   Q8XZJ6
- GeneID:   1220223
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1399
- NMPDR:   fig|267608.1.peg.1399
- HOGENOM:   HBG665227
- OMA:   HNERYPD
- ProtClustDB:   PRK12846
- BioCyc:   RSOL267608:RSC1399-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19950; Mature: 19950

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPILKMGDSRLLRVAKPVQRFQTPELTALIEDMFDTMDAARGAGLAAPQIGVDLQVVI
CCCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
FGFDRNDRYPDAPAVPKTVLINPTIEPLSDAMEDGWEGCLSVPGLRGVVPRYTRLRYTGY
EEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEECCC
DQHGHAIDRIAEGFHARVVQHECDHLQGILYPMRVQDFTRFGFTEILFPELPAHHND
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIRPILKMGDSRLLRVAKPVQRFQTPELTALIEDMFDTMDAARGAGLAAPQIGVDLQVVI
CCCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEE
FGFDRNDRYPDAPAVPKTVLINPTIEPLSDAMEDGWEGCLSVPGLRGVVPRYTRLRYTGY
EEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEECCC
DQHGHAIDRIAEGFHARVVQHECDHLQGILYPMRVQDFTRFGFTEILFPELPAHHND
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852