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The map label for this gene is rlmN [H]

Identifier: 17545931

GI number: 17545931

Start: 1280644

End: 1281795

Strand: Direct

Name: rlmN [H]

Synonym: RSc1212

Alternate gene names: 17545931

Gene position: 1280644-1281795 (Clockwise)

Preceding gene: 17545930

Following gene: 17545932

Centisome position: 34.46

GC content: 64.15

Gene sequence:

>1152_bases
ATGAACGATATGGTGAATCTTCTTGATTTCGACGCACAGGGCCTGCTCGCATATTGCGAGAGCCTGGGCGAGAAGTCGTT
CCGCGCCAAGCAGCTGCAGCGCTGGATTCACCAGTCGGGCGCCGCCGATTTTGGCGAGATGACCGATCTCGCCAAGTCCC
TGCGCGAGAAGCTTGCGACCCGCGCCACCATCCAGGCGCCGGCGGTCATCTCCGACCACCTGTCGTCGGACGGCACGCGC
AAGTGGCTGGTCGACGTGGGTGCCGGCAATGCCGTCGAGACGGTGTACATCCCCGAGGAGACGCGCGGCACGCTGTGCGT
GTCTTCGCAGGCGGGGTGCGCCGTCAACTGCCGGTTCTGTTCGACCGGCAAGCAGGGGTTCTCGCGCAACCTCAGCACAG
GCGAGATCGTCGGCCAGCTGTGGATGGCCGAGTTCGCCATGCGCAAGCAGCTCGGCCGCGGCCCCAAGGATGACCGCGTC
ATCACAAACGTCGTGATGATGGGCATGGGCGAGCCGCTGCTCAACTATGACGCCGTGGTGCCGGCCATGCGCCTGATGCT
GGATGACAATGCCTATGGCCTGTCGCGCCGGCGCGTGACGCTGTCCACTTCCGGCGTGGTGCCGATGATGGACCGGCTGT
CGCAAGATTTGCCGGTGGCGCTGGCGGTTTCGCTGCATGCCTCCAACGATGCGCTGCGCGATGTGCTGGTGCCACTCAAC
AAGAAGTATCCGCTGGCCGAACTGATGGCGGCCTGCCGCCGCTATCTCGAATTCGCGCCGCGGGACTTCATCACCTTCGA
ATACTGCATGCTGGACGGCGTCAATGATTCCGTCGAGCATGCGCGCGAACTGCTGCGCGTCGTTGCCGACGTGCCGTGCA
AGTTCAACCTGATCCCGTTCAACCCGTTCCCCGAATCGGGCCTGAAGCGCTCGAACAACGAGCAGATTCGCCGGTTCTCG
CAGGTGCTGCTGGACGCGGGCATCGTCACCACGATCCGCAAGACGCGCGGTGACGATATCGACGCGGCCTGCGGCCAGTT
GGCCGGCGAGGTCAAGGATCGTACGCGCCTGGCCGAGCGCGGTAAATTCGGCAAGACTGTTCCGATCCAGGTGATCGGGG
CCGATTCCACTCACCGCATGGGTTCCGCATGA

Upstream 100 bases:

>100_bases
ACGGCTGCCGTGGAAGTCGCTTTCTTCTTCCCCGGCCTGAACATTTACAGCCGCTAAGCGTCAGCACTAGACTGGCATTG
GCGGTTTCGGGCAGCAAGTC

Downstream 100 bases:

>100_bases
AGCGTTGGTCGACTGCTGTCAGTCTGGTTTTCACACTGGCCGCCGCCGGGTGCGCACTGCCGCCGCCACCGGCCCAGACG
CAGGACGTCAAGACGCTCTC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNDMVNLLDFDAQGLLAYCESLGEKSFRAKQLQRWIHQSGAADFGEMTDLAKSLREKLATRATIQAPAVISDHLSSDGTR
KWLVDVGAGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIVGQLWMAEFAMRKQLGRGPKDDRV
ITNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSQDLPVALAVSLHASNDALRDVLVPLN
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDSVEHARELLRVVADVPCKFNLIPFNPFPESGLKRSNNEQIRRFS
QVLLDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAERGKFGKTVPIQVIGADSTHRMGSA

Sequences:

>Translated_383_residues
MNDMVNLLDFDAQGLLAYCESLGEKSFRAKQLQRWIHQSGAADFGEMTDLAKSLREKLATRATIQAPAVISDHLSSDGTR
KWLVDVGAGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIVGQLWMAEFAMRKQLGRGPKDDRV
ITNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSQDLPVALAVSLHASNDALRDVLVPLN
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDSVEHARELLRVVADVPCKFNLIPFNPFPESGLKRSNNEQIRRFS
QVLLDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAERGKFGKTVPIQVIGADSTHRMGSA
>Mature_383_residues
MNDMVNLLDFDAQGLLAYCESLGEKSFRAKQLQRWIHQSGAADFGEMTDLAKSLREKLATRATIQAPAVISDHLSSDGTR
KWLVDVGAGNAVETVYIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIVGQLWMAEFAMRKQLGRGPKDDRV
ITNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSQDLPVALAVSLHASNDALRDVLVPLN
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDSVEHARELLRVVADVPCKFNLIPFNPFPESGLKRSNNEQIRRFS
QVLLDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAERGKFGKTVPIQVIGADSTHRMGSA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=373, Percent_Identity=54.6916890080429, Blast_Score=403, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 42185; Mature: 42185

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDMVNLLDFDAQGLLAYCESLGEKSFRAKQLQRWIHQSGAADFGEMTDLAKSLREKLAT
CCCHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RATIQAPAVISDHLSSDGTRKWLVDVGAGNAVETVYIPEETRGTLCVSSQAGCAVNCRFC
HHHHCCCHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEEEEE
STGKQGFSRNLSTGEIVGQLWMAEFAMRKQLGRGPKDDRVITNVVMMGMGEPLLNYDAVV
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
PAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSQDLPVALAVSLHASNDALRDVLVPLN
HHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHCCC
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDSVEHARELLRVVADVPCKFNLIPF
CCCCHHHHHHHHHHHHHHCCCHHEEEEHHEECCCCHHHHHHHHHHHHHHCCCCEEEEEEC
NPFPESGLKRSNNEQIRRFSQVLLDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAER
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GKFGKTVPIQVIGADSTHRMGSA
CCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MNDMVNLLDFDAQGLLAYCESLGEKSFRAKQLQRWIHQSGAADFGEMTDLAKSLREKLAT
CCCHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RATIQAPAVISDHLSSDGTRKWLVDVGAGNAVETVYIPEETRGTLCVSSQAGCAVNCRFC
HHHHCCCHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEEEEE
STGKQGFSRNLSTGEIVGQLWMAEFAMRKQLGRGPKDDRVITNVVMMGMGEPLLNYDAVV
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
PAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSQDLPVALAVSLHASNDALRDVLVPLN
HHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHCCC
KKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDSVEHARELLRVVADVPCKFNLIPF
CCCCHHHHHHHHHHHHHHCCCHHEEEEHHEECCCCHHHHHHHHHHHHHHCCCCEEEEEEC
NPFPESGLKRSNNEQIRRFSQVLLDAGIVTTIRKTRGDDIDAACGQLAGEVKDRTRLAER
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GKFGKTVPIQVIGADSTHRMGSA
CCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA