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The map label for this gene is pgk

Identifier: 17545290

GI number: 17545290

Start: 614382

End: 615641

Strand: Direct

Name: pgk

Synonym: RSc0571

Alternate gene names: 17545290

Gene position: 614382-615641 (Clockwise)

Preceding gene: 17545289

Following gene: 17545291

Centisome position: 16.53

GC content: 66.9

Gene sequence:

>1260_bases
GTGGCATCGGGTAAAATCGCCGCTTTCCGTGATTTCTCCGCTGCTGCCATGCCCAACGTCCTGCGTCTGACCGATCTCAT
CTCCGAAGGCAAGCTTGCCGGCAAGCGTGTCTTCATCCGCGCCGATCTCAACGTGCCGCAAGACGATGCCGGCAACATCA
CCGAAGACACGCGCATCCGCGCTTCGGTGCCGGCCATCCGCGCCGCGCTCGACGCCGGTGCCGCCGTGATGGTCACCTCG
CATCTGGGCCGTCCGACCGAAGGCGAGTTCAAGCCGGAAGATTCGCTGGCCCCGGTGGCCGTGCGCCTGTCCGAGTTGCT
CGGCTGTGAGGTGAAGCTGGTGCAGAACTGGGTCGACGGCGTGGATGTCGCGCCCGGCCAGGTGGTGCTGCTGGAAAACT
GCCGCGTCAACAAGGGCGAGAAGAAGAACAGCGACGAACTGGCCCAGAAGATGGCCAAGCTGTGCGACGTCTACGTCAAC
GACGCCTTCGGCACCGCCCACCGCGCCGAGGCCACCACGCACGGCATCGCCAAGTTTGCCCCCATCGCCTGCGCCGGCCC
GCTGCTGGGCGCCGAGCTGGACGCGCTGGGCAAGGCGCTGGGCCAACCGGCCCGTCCGCTGGTCGCCATCGTGGCCGGCT
CCAAGGTGTCGACCAAGCTGACCATCCTGAAATCGCTGGCCGACAAGGTGGACAACCTGATCGTCGGCGGCGGCATCGCC
AACACGTTCATGCTGGCCGCGGGCCTGAAGATCGGCAAGTCGCTGGCCGAGGCGGATCTGGTGGGCGATGCCAAGGCGAT
CATCGACCTGATGGCTGCCCGTGGCGCCTCGGTGCCCATTCCTGTCGATGTGGTCTGCGCCAAGGAATTCAGCGCCACCG
CTGCCGCCACCGTCAAGGACGTGAAGGACGTGGCGGATGACGACATGATTCTCGACATCGGCCCGAAGACCGCCGCGCAA
CTGGCCGACCAGCTCAAGGCCTCCGGCACCATCGTGTGGAACGGCCCGGTCGGCGTGTTCGAGTTCGACCAGTTCGGCAA
CGGCACCAAGGTGCTGGCCGAGGCGATCGCGGCCTCGTCGGGCTTCTCGATCGCCGGTGGCGGCGATACGCTGGCGGCGA
TCGCCAAGTACGGCATCGCCGACCGCGTGGGCTACATCTCCACCGGCGGCGGCGCATTCCTGGAATTCCTGGAAGGCAAG
ACGCTGCCGGCCGTGGAGATCCTGGAGCAGCGTGCCCAGCGCCAGGAAGCGCTGGCCTGA

Upstream 100 bases:

>100_bases
CGCGCCGGTGGCGGCCTGACCGGCGCGTGCGCCATCCAGCCGGCTTCGCCGCGATGGACGGGCGCGTCAGGTTTCCGGGC
GGAAACGCGCGTTCCGACCG

Downstream 100 bases:

>100_bases
AGCGCCAGGCACGTCGCCTGCGTCGTCAACGTCCCCCAAACGTCACCGGTACCCCTTGCCCATGACTCGCGCCACCAAGA
TCGTCGCCACACTCGGCCCT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MASGKIAAFRDFSAAAMPNVLRLTDLISEGKLAGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIRAALDAGAAVMVTS
HLGRPTEGEFKPEDSLAPVAVRLSELLGCEVKLVQNWVDGVDVAPGQVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVN
DAFGTAHRAEATTHGIAKFAPIACAGPLLGAELDALGKALGQPARPLVAIVAGSKVSTKLTILKSLADKVDNLIVGGGIA
NTFMLAAGLKIGKSLAEADLVGDAKAIIDLMAARGASVPIPVDVVCAKEFSATAAATVKDVKDVADDDMILDIGPKTAAQ
LADQLKASGTIVWNGPVGVFEFDQFGNGTKVLAEAIAASSGFSIAGGGDTLAAIAKYGIADRVGYISTGGGAFLEFLEGK
TLPAVEILEQRAQRQEALA

Sequences:

>Translated_419_residues
MASGKIAAFRDFSAAAMPNVLRLTDLISEGKLAGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIRAALDAGAAVMVTS
HLGRPTEGEFKPEDSLAPVAVRLSELLGCEVKLVQNWVDGVDVAPGQVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVN
DAFGTAHRAEATTHGIAKFAPIACAGPLLGAELDALGKALGQPARPLVAIVAGSKVSTKLTILKSLADKVDNLIVGGGIA
NTFMLAAGLKIGKSLAEADLVGDAKAIIDLMAARGASVPIPVDVVCAKEFSATAAATVKDVKDVADDDMILDIGPKTAAQ
LADQLKASGTIVWNGPVGVFEFDQFGNGTKVLAEAIAASSGFSIAGGGDTLAAIAKYGIADRVGYISTGGGAFLEFLEGK
TLPAVEILEQRAQRQEALA
>Mature_418_residues
ASGKIAAFRDFSAAAMPNVLRLTDLISEGKLAGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIRAALDAGAAVMVTSH
LGRPTEGEFKPEDSLAPVAVRLSELLGCEVKLVQNWVDGVDVAPGQVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVND
AFGTAHRAEATTHGIAKFAPIACAGPLLGAELDALGKALGQPARPLVAIVAGSKVSTKLTILKSLADKVDNLIVGGGIAN
TFMLAAGLKIGKSLAEADLVGDAKAIIDLMAARGASVPIPVDVVCAKEFSATAAATVKDVKDVADDDMILDIGPKTAAQL
ADQLKASGTIVWNGPVGVFEFDQFGNGTKVLAEAIAASSGFSIAGGGDTLAAIAKYGIADRVGYISTGGGAFLEFLEGKT
LPAVEILEQRAQRQEALA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=410, Percent_Identity=42.4390243902439, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI4505763, Length=425, Percent_Identity=40, Blast_Score=257, Evalue=2e-68,
Organism=Escherichia coli, GI1789294, Length=396, Percent_Identity=63.1313131313131, Blast_Score=483, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17508823, Length=409, Percent_Identity=43.2762836185819, Blast_Score=286, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6319857, Length=406, Percent_Identity=41.1330049261084, Blast_Score=262, Evalue=6e-71,
Organism=Drosophila melanogaster, GI17136394, Length=404, Percent_Identity=41.5841584158416, Blast_Score=254, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24581203, Length=408, Percent_Identity=38.4803921568627, Blast_Score=234, Evalue=8e-62,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_RALSO (Q8Y1W6)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518692.1
- ProteinModelPortal:   Q8Y1W6
- SMR:   Q8Y1W6
- GeneID:   1219376
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0571
- NMPDR:   fig|267608.1.peg.571
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- ProtClustDB:   PRK00073
- BioCyc:   RSOL267608:RSC0571-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43269; Mature: 43138

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 58-58 BINDING 135-135 BINDING 168-168 BINDING 219-219 BINDING 341-341

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGKIAAFRDFSAAAMPNVLRLTDLISEGKLAGKRVFIRADLNVPQDDAGNITEDTRIR
CCCCCEEEECCCCHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEE
ASVPAIRAALDAGAAVMVTSHLGRPTEGEFKPEDSLAPVAVRLSELLGCEVKLVQNWVDG
HHHHHHHHHHHCCCEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCC
VDVAPGQVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIAKFA
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
PIACAGPLLGAELDALGKALGQPARPLVAIVAGSKVSTKLTILKSLADKVDNLIVGGGIA
CHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCEEECCCHH
NTFMLAAGLKIGKSLAEADLVGDAKAIIDLMAARGASVPIPVDVVCAKEFSATAAATVKD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHH
VKDVADDDMILDIGPKTAAQLADQLKASGTIVWNGPVGVFEFDQFGNGTKVLAEAIAASS
HHHHCCCCEEEEECCHHHHHHHHHHHHCCEEEECCCCCEEEHHCCCCHHHHHHHHHHHCC
GFSIAGGGDTLAAIAKYGIADRVGYISTGGGAFLEFLEGKTLPAVEILEQRAQRQEALA
CCEEECCCHHHHHHHHHCHHHCCCEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASGKIAAFRDFSAAAMPNVLRLTDLISEGKLAGKRVFIRADLNVPQDDAGNITEDTRIR
CCCCEEEECCCCHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEE
ASVPAIRAALDAGAAVMVTSHLGRPTEGEFKPEDSLAPVAVRLSELLGCEVKLVQNWVDG
HHHHHHHHHHHCCCEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCC
VDVAPGQVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIAKFA
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
PIACAGPLLGAELDALGKALGQPARPLVAIVAGSKVSTKLTILKSLADKVDNLIVGGGIA
CHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCEEECCCHH
NTFMLAAGLKIGKSLAEADLVGDAKAIIDLMAARGASVPIPVDVVCAKEFSATAAATVKD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHH
VKDVADDDMILDIGPKTAAQLADQLKASGTIVWNGPVGVFEFDQFGNGTKVLAEAIAASS
HHHHCCCCEEEEECCHHHHHHHHHHHHCCEEEECCCCCEEEHHCCCCHHHHHHHHHHHCC
GFSIAGGGDTLAAIAKYGIADRVGYISTGGGAFLEFLEGKTLPAVEILEQRAQRQEALA
CCEEECCCHHHHHHHHHCHHHCCCEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852