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The map label for this gene is miaB

Identifier: 17545250

GI number: 17545250

Start: 569640

End: 571013

Strand: Reverse

Name: miaB

Synonym: RSc0531

Alternate gene names: 17545250

Gene position: 571013-569640 (Counterclockwise)

Preceding gene: 17545251

Following gene: 17545249

Centisome position: 15.36

GC content: 66.81

Gene sequence:

>1374_bases
ATGAAAAAAGTCTTCATCAAAACCTTCGGCTGCCAGATGAACGAGTACGACTCGGACAAGATGGCCGACGTTCTCAACGC
CGCTGAAGGTCTCGTCCCCACCGACACGCCCGAAGACGCGGACGTGATCCTCTTCAACACCTGCTCGGTGCGCGAGAAGG
CGCAGGAGAAAGTGTTCTCCGACCTCGGCCGCGTCAAGGCGCTCAAGGCGCGCAACCCGGACCTCGTGGTCGGCGTGGGC
GGCTGCGTGGCGAGCCAGGAAGGCGCCTCCATCGTGGCGCGCGCGCCCTACGTCGACGTGGTCTTCGGCCCGCAGACGCT
GCACCGCCTGCCGGAGCTGATCGACGCGCGCCGCCGCACGGGCCGCCCGCAGGTGGACGTGTCCTTCCCCGAGATCGAGA
AGTTCGACCACCTGCCGCCCGCGCGCGTGGAAGGCCCGAGCGCGTTCGTCTCGATCATGGAGGGCTGCTCGAAATACTGC
AGCTACTGCGTGGTGCCTTACACGCGCGGCGAAGAGGTGTCGCGCCCGTTCGAGGATGTGCTGGCCGAGGTGGCCGGCCT
GGCCGAGCAGGGCGTGCGCGAAGTCACGCTGCTGGGCCAGAACGTCAACGCCTACATCGGCAAGATGGGCGGCACCAGCG
AGCGTGCGGACTTTGCCCTGCTGCTCGAATACGTGGCCGAGATTCCCGGCATCGAGCGCATCCGCTACACCACCAGCCAC
CCGAAGGAATTCACCGCGCGCCTGATCGAGGCCTACGCCACCAACCGCAAGCTGGTCGACCACCTGCACCTGCCCGTGCA
GCACGGCTCCGACCGCATCCTGATGGCGATGAAGCGCGGCTACACGGTGCTCGAGTACAAGAGCATCATCCGCAAGCTCC
GCGCGATCCGGCCGGACATCTCGATCGCCACCGACTTCATCGTCGGCTTCCCCGGCGAGACCGATGCGGACTTCGCCAAG
ACCATGGACCTCGTCCACGAGATCGGCTATGACAACTCGTTCAGCTTCATCTACAGCCCGCGCCCCGGCACGCCGGCCGC
CAATCTGCACGACGACACGCCGCACGCCGTCAAGCTCGAGCGCCTGAAGCACCTGCAGGCGACCATCGACGCCAACATGG
CGCGCATCAGCGAAGGCATGGTCGGCAGCGTGCAGCGCATCCTGGTCGAAGGCCCGTCGCGCAAGGACCCGAGCGAGCTG
CACGGCCGCACCGAGAACAACCGCGTGGTCAACTTCGCCCTGCCCGATCTGCCGCAGGCGCGCCGCGACCAGCTGGTCGG
CCAGATGCTGGACGTGCGCATCGTGCATGCGTATCCGCACTCGCTGCGCGGGGACGTGGTTGAACAGCGCCCGGACGGCG
TGACCCAGCACTAG

Upstream 100 bases:

>100_bases
CGACGACGAGTACAAGGCGGCGGCGAGCAGCCAGTAAGCCAACCGGGGGCCACCCGCCCCGCGAAGCACGTGCCCCCAGG
TCAACCACTGCGAACGGCAC

Downstream 100 bases:

>100_bases
GCACACCCATCTTGAACAAAGCGAAACCCCACGAGTTCGTCGCCCCGCGCGACGACAACGCGCGCCTGCAGAACCTGTGC
GGGCCGCTCGATGAAAACCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKKVFIKTFGCQMNEYDSDKMADVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFSDLGRVKALKARNPDLVVGVG
GCVASQEGASIVARAPYVDVVFGPQTLHRLPELIDARRRTGRPQVDVSFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYC
SYCVVPYTRGEEVSRPFEDVLAEVAGLAEQGVREVTLLGQNVNAYIGKMGGTSERADFALLLEYVAEIPGIERIRYTTSH
PKEFTARLIEAYATNRKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSIIRKLRAIRPDISIATDFIVGFPGETDADFAK
TMDLVHEIGYDNSFSFIYSPRPGTPAANLHDDTPHAVKLERLKHLQATIDANMARISEGMVGSVQRILVEGPSRKDPSEL
HGRTENNRVVNFALPDLPQARRDQLVGQMLDVRIVHAYPHSLRGDVVEQRPDGVTQH

Sequences:

>Translated_457_residues
MKKVFIKTFGCQMNEYDSDKMADVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFSDLGRVKALKARNPDLVVGVG
GCVASQEGASIVARAPYVDVVFGPQTLHRLPELIDARRRTGRPQVDVSFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYC
SYCVVPYTRGEEVSRPFEDVLAEVAGLAEQGVREVTLLGQNVNAYIGKMGGTSERADFALLLEYVAEIPGIERIRYTTSH
PKEFTARLIEAYATNRKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSIIRKLRAIRPDISIATDFIVGFPGETDADFAK
TMDLVHEIGYDNSFSFIYSPRPGTPAANLHDDTPHAVKLERLKHLQATIDANMARISEGMVGSVQRILVEGPSRKDPSEL
HGRTENNRVVNFALPDLPQARRDQLVGQMLDVRIVHAYPHSLRGDVVEQRPDGVTQH
>Mature_457_residues
MKKVFIKTFGCQMNEYDSDKMADVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFSDLGRVKALKARNPDLVVGVG
GCVASQEGASIVARAPYVDVVFGPQTLHRLPELIDARRRTGRPQVDVSFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYC
SYCVVPYTRGEEVSRPFEDVLAEVAGLAEQGVREVTLLGQNVNAYIGKMGGTSERADFALLLEYVAEIPGIERIRYTTSH
PKEFTARLIEAYATNRKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSIIRKLRAIRPDISIATDFIVGFPGETDADFAK
TMDLVHEIGYDNSFSFIYSPRPGTPAANLHDDTPHAVKLERLKHLQATIDANMARISEGMVGSVQRILVEGPSRKDPSEL
HGRTENNRVVNFALPDLPQARRDQLVGQMLDVRIVHAYPHSLRGDVVEQRPDGVTQH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=36.7219917012448, Blast_Score=298, Evalue=5e-81,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=36.7219917012448, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI93277076, Length=471, Percent_Identity=29.5116772823779, Blast_Score=172, Evalue=6e-43,
Organism=Escherichia coli, GI1786882, Length=448, Percent_Identity=63.1696428571429, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1787057, Length=466, Percent_Identity=28.9699570815451, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=468, Percent_Identity=34.8290598290598, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI71996771, Length=301, Percent_Identity=30.2325581395349, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=34.4329896907216, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI19922432, Length=475, Percent_Identity=29.6842105263158, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_RALSO (Q8Y206)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518652.1
- ProteinModelPortal:   Q8Y206
- SMR:   Q8Y206
- GeneID:   1219335
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0531
- NMPDR:   fig|267608.1.peg.531
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- BioCyc:   RSOL267608:RSC0531-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50732; Mature: 50732

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVFIKTFGCQMNEYDSDKMADVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFS
CCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
DLGRVKALKARNPDLVVGVGGCVASQEGASIVARAPYVDVVFGPQTLHRLPELIDARRRT
HHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHHC
GRPQVDVSFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV
CCCCEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHCCCHHHH
LAEVAGLAEQGVREVTLLGQNVNAYIGKMGGTSERADFALLLEYVAEIPGIERIRYTTSH
HHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
PKEFTARLIEAYATNRKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSIIRKLRAIRPDI
CHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCCCH
SIATDFIVGFPGETDADFAKTMDLVHEIGYDNSFSFIYSPRPGTPAANLHDDTPHAVKLE
HHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHH
RLKHLQATIDANMARISEGMVGSVQRILVEGPSRKDPSELHGRTENNRVVNFALPDLPQA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCHH
RRDQLVGQMLDVRIVHAYPHSLRGDVVEQRPDGVTQH
HHHHHHHHHHCEEEEECCCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MKKVFIKTFGCQMNEYDSDKMADVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFS
CCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
DLGRVKALKARNPDLVVGVGGCVASQEGASIVARAPYVDVVFGPQTLHRLPELIDARRRT
HHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEECCEEEEEECHHHHHHHHHHHHHHHHC
GRPQVDVSFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV
CCCCEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHCCCHHHH
LAEVAGLAEQGVREVTLLGQNVNAYIGKMGGTSERADFALLLEYVAEIPGIERIRYTTSH
HHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
PKEFTARLIEAYATNRKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSIIRKLRAIRPDI
CHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCCCH
SIATDFIVGFPGETDADFAKTMDLVHEIGYDNSFSFIYSPRPGTPAANLHDDTPHAVKLE
HHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHH
RLKHLQATIDANMARISEGMVGSVQRILVEGPSRKDPSELHGRTENNRVVNFALPDLPQA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCHH
RRDQLVGQMLDVRIVHAYPHSLRGDVVEQRPDGVTQH
HHHHHHHHHHCEEEEECCCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852