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The map label for this gene is glyS

Identifier: 17545243

GI number: 17545243

Start: 561288

End: 563381

Strand: Reverse

Name: glyS

Synonym: RSc0524

Alternate gene names: 17545243

Gene position: 563381-561288 (Counterclockwise)

Preceding gene: 17545244

Following gene: 17545242

Centisome position: 15.16

GC content: 69.53

Gene sequence:

>2094_bases
ATGTCCACCCTCCTGATCGAACTGCTGACCGAAGAACTGCCGCCGAAGGCGCTGGCCCGCCTGGGCGAGGCGTTTGCGCA
GAGCCTGTTCGACGGCCTGTCGGCGCAAGGCCTGCTCGAGGAAGGCGCGCAGGTGGAAGGCTTTGCCACCCCGCGCCGCC
TGGCCGCGTCGATCACCGGCGTGCGCCGCGCCGCGCCCGACCGCGAGCTGCGCGAGAAGGTGCTGCCCGTCAACATCGCC
TTCGACGCCGAAGGCAAGCCGACCGCGCCGCTGACCAAGAAGCTCGCGGCGCTCGCCAAGTCGATCGGCGCGGACACGAT
CGCGCCCGAATCGCTGGAGCGCGCGCCGGACGGCAAGGCCGAATCGCTGTTCCACCGCTACACCGCGCGCGGCGCTGTGC
TGGCCGATGGCCTGCAGGCCGCGCTGTCGCAAACCATCGCCGGCCTGCCGATCCCCAAGGTGATGATCTACCAGCGCCCC
AACGGCGACAACGTGCAGTTCGTGCGCCCGGCGCACCGCCTGATCGCGCTGCTGGACGACGAAATCATCCCCGCCGGCGT
GCTGGGCCTGCAGTCGGGCAACGTCACGCTGGGCCACCGCTTCCTGTCGGCCGGCGAGATCATCATTCCGCACGCCACCG
CCTATGCGAGCACGCTGAAGTCGCAGGGCAAGGTCATCGCCGGCTATGCCGAACGCAAGGAAGCCATCCGCGCCGAACTG
CTGAAGGCCGCCGGCGCCGATACGGTGGTGATGCCCGAGGCGCTGCTCGACGAGGTCAATGCGCTGGTCGAATGGCCGGT
GGTCTACCCCTGCCACTTCGAGGAACAGTTCCTCGCCGTGCCGCAGGAGTGCCTGATCCTGACCATGCAGACCAACCAGA
AGTACTTTGCGCTGACCGACGCGCAGGGCCATCTGCGCAACCGCTTCCTGATCGTCTCCAACCTGGCCACCGAGACGCCG
CAGGCCATCATCGAAGGCAACGAGCGCGTGGTGCGCCCGCGCCTGGCCGATGCCCGCTTTTTCTTCGAGCACGACAAGAA
GAAGCCGCTGGCCGATCGCGTGCCGCAGCTCGCGCGCGTGGTCTACCACAACAAGATCGGCACGCAGCTCGAGCGCGTCT
CGCGCCTGCAGGCGATCGCCGGCCAGCTGGCCGAGAAGCTCGGCGCGGAAGTCGCGCACGCCTCGCGCGCGGCGCTGCTG
GCCAAGGCCGACCTGCTGACCGACATGGTCGGCGAGTTCCCCGAGCTGCAGGGCACCATGGGCACCTACTACGCCCGCCA
CGACGGCGAGGCCGAAGACGTGGCGCTGGCCTGCTCCGAGCACTATCAGCCGCGCTTCGCCGGCGACGCGCTGCCCGGCA
CCGCCACCGGCACCGTGGTGGCGCTGGCCGACAAGCTGGAGACGCTGGTCGGCATCTGGGGCATCGGCCTGGCGCCCACC
GGCGAGAAAGACCCGTTCGCCCTGCGCCGCCATGCGCTGGGCATCCTGCGCATGCTGATCGAGAAGCCGCTGGCGCTGGG
CATCGCCGAAGTGCTGGAGGCAGCCGCCGCCAGCTTCGAGGGCATCGCCGCCGTCAAGCCGGACCTGGCCGCCATCACCG
ACTTCCTGTATGACCGCCTGCGCGGCTACCTGAAGGACAAGGGCTACAGCACCAACGAGGTGGAGGCCGTGGTCAGCCAG
CGCCCGCAGCGCCTGGACGACATCGTCGCGCGCCTGGAAGCCGTGCGCGCCTTCGCCGCCCTGCCGCAGGCCGAAGCGCT
GGCCGCCGCCAACAAGCGCATCACCAACATCCTGAAGAAGACCGACATCACCATCGGCTCGGTGCAGCCGCAGTTGCTCA
GGGAAGACGCCGAGCGCGCGCTGCACCAAGCCGTCGCCACCTCGGAGCCGCACGTGCACGATGCCTTCGCGCGCGGCGAC
TTCACCACCGCGCTCAAGACGCTGGCCAGCCTGCGCGAAGCGGTCGACAGCTTCTTCGACGGCGTGATGGTGATGGCCGA
CGACACCGCGCTGCGCGACAACCGCCTGGCCCTGCTGGGCGAGCTGCACGGCCTGATGAACCGCGTGGCCGACATCTCCA
AGCTGGCAGCGTAA

Upstream 100 bases:

>100_bases
GTCGGGCGTGGAGCCCGTGCCGGGCTCGCCCTGCTGAACCCAACACCGTAACCGCCGCCCGCGCGCGGCGCAGAACAACG
ACGTCTCTGATTCTTACGCT

Downstream 100 bases:

>100_bases
GCCCTGCCCCGGAGCCCGACATGCCGCACATACCGAAGCTCGTGATCCTGGACCGCGATGGCGTCATCAACCTCGACAGC
GACCAGTTCATCAAGTCGCC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MSTLLIELLTEELPPKALARLGEAFAQSLFDGLSAQGLLEEGAQVEGFATPRRLAASITGVRRAAPDRELREKVLPVNIA
FDAEGKPTAPLTKKLAALAKSIGADTIAPESLERAPDGKAESLFHRYTARGAVLADGLQAALSQTIAGLPIPKVMIYQRP
NGDNVQFVRPAHRLIALLDDEIIPAGVLGLQSGNVTLGHRFLSAGEIIIPHATAYASTLKSQGKVIAGYAERKEAIRAEL
LKAAGADTVVMPEALLDEVNALVEWPVVYPCHFEEQFLAVPQECLILTMQTNQKYFALTDAQGHLRNRFLIVSNLATETP
QAIIEGNERVVRPRLADARFFFEHDKKKPLADRVPQLARVVYHNKIGTQLERVSRLQAIAGQLAEKLGAEVAHASRAALL
AKADLLTDMVGEFPELQGTMGTYYARHDGEAEDVALACSEHYQPRFAGDALPGTATGTVVALADKLETLVGIWGIGLAPT
GEKDPFALRRHALGILRMLIEKPLALGIAEVLEAAAASFEGIAAVKPDLAAITDFLYDRLRGYLKDKGYSTNEVEAVVSQ
RPQRLDDIVARLEAVRAFAALPQAEALAAANKRITNILKKTDITIGSVQPQLLREDAERALHQAVATSEPHVHDAFARGD
FTTALKTLASLREAVDSFFDGVMVMADDTALRDNRLALLGELHGLMNRVADISKLAA

Sequences:

>Translated_697_residues
MSTLLIELLTEELPPKALARLGEAFAQSLFDGLSAQGLLEEGAQVEGFATPRRLAASITGVRRAAPDRELREKVLPVNIA
FDAEGKPTAPLTKKLAALAKSIGADTIAPESLERAPDGKAESLFHRYTARGAVLADGLQAALSQTIAGLPIPKVMIYQRP
NGDNVQFVRPAHRLIALLDDEIIPAGVLGLQSGNVTLGHRFLSAGEIIIPHATAYASTLKSQGKVIAGYAERKEAIRAEL
LKAAGADTVVMPEALLDEVNALVEWPVVYPCHFEEQFLAVPQECLILTMQTNQKYFALTDAQGHLRNRFLIVSNLATETP
QAIIEGNERVVRPRLADARFFFEHDKKKPLADRVPQLARVVYHNKIGTQLERVSRLQAIAGQLAEKLGAEVAHASRAALL
AKADLLTDMVGEFPELQGTMGTYYARHDGEAEDVALACSEHYQPRFAGDALPGTATGTVVALADKLETLVGIWGIGLAPT
GEKDPFALRRHALGILRMLIEKPLALGIAEVLEAAAASFEGIAAVKPDLAAITDFLYDRLRGYLKDKGYSTNEVEAVVSQ
RPQRLDDIVARLEAVRAFAALPQAEALAAANKRITNILKKTDITIGSVQPQLLREDAERALHQAVATSEPHVHDAFARGD
FTTALKTLASLREAVDSFFDGVMVMADDTALRDNRLALLGELHGLMNRVADISKLAA
>Mature_696_residues
STLLIELLTEELPPKALARLGEAFAQSLFDGLSAQGLLEEGAQVEGFATPRRLAASITGVRRAAPDRELREKVLPVNIAF
DAEGKPTAPLTKKLAALAKSIGADTIAPESLERAPDGKAESLFHRYTARGAVLADGLQAALSQTIAGLPIPKVMIYQRPN
GDNVQFVRPAHRLIALLDDEIIPAGVLGLQSGNVTLGHRFLSAGEIIIPHATAYASTLKSQGKVIAGYAERKEAIRAELL
KAAGADTVVMPEALLDEVNALVEWPVVYPCHFEEQFLAVPQECLILTMQTNQKYFALTDAQGHLRNRFLIVSNLATETPQ
AIIEGNERVVRPRLADARFFFEHDKKKPLADRVPQLARVVYHNKIGTQLERVSRLQAIAGQLAEKLGAEVAHASRAALLA
KADLLTDMVGEFPELQGTMGTYYARHDGEAEDVALACSEHYQPRFAGDALPGTATGTVVALADKLETLVGIWGIGLAPTG
EKDPFALRRHALGILRMLIEKPLALGIAEVLEAAAASFEGIAAVKPDLAAITDFLYDRLRGYLKDKGYSTNEVEAVVSQR
PQRLDDIVARLEAVRAFAALPQAEALAAANKRITNILKKTDITIGSVQPQLLREDAERALHQAVATSEPHVHDAFARGDF
TTALKTLASLREAVDSFFDGVMVMADDTALRDNRLALLGELHGLMNRVADISKLAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=700, Percent_Identity=45.8571428571429, Blast_Score=565, Evalue=1e-162,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_RALSO (Q8Y213)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518645.1
- ProteinModelPortal:   Q8Y213
- GeneID:   1219328
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0524
- NMPDR:   fig|267608.1.peg.524
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- BioCyc:   RSOL267608:RSC0524-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75258; Mature: 75127

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLLIELLTEELPPKALARLGEAFAQSLFDGLSAQGLLEEGAQVEGFATPRRLAASITG
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHH
VRRAAPDRELREKVLPVNIAFDAEGKPTAPLTKKLAALAKSIGADTIAPESLERAPDGKA
HHHCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHH
ESLFHRYTARGAVLADGLQAALSQTIAGLPIPKVMIYQRPNGDNVQFVRPAHRLIALLDD
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEECHHHHHHHHHCC
EIIPAGVLGLQSGNVTLGHRFLSAGEIIIPHATAYASTLKSQGKVIAGYAERKEAIRAEL
CCCCCHHEEECCCCEEHHHHHHCCCCEEECCHHHHHHHHHHCCCEEECHHHHHHHHHHHH
LKAAGADTVVMPEALLDEVNALVEWPVVYPCHFEEQFLAVPQECLILTMQTNQKYFALTD
HHHCCCCCEECCHHHHHHHHHHHHCCEEECCCCCHHHHHCCHHHEEEEEECCCEEEEEEC
AQGHLRNRFLIVSNLATETPQAIIEGNERVVRPRLADARFFFEHDKKKPLADRVPQLARV
CCHHHHCCEEEEECCCCCCCHHHHCCCCCEECCCHHHHHHHHHCCCCCCHHHHHHHHHHH
VYHNKIGTQLERVSRLQAIAGQLAEKLGAEVAHASRAALLAKADLLTDMVGEFPELQGTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCH
GTYYARHDGEAEDVALACSEHYQPRFAGDALPGTATGTVVALADKLETLVGIWGIGLAPT
HHEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GEKDPFALRRHALGILRMLIEKPLALGIAEVLEAAAASFEGIAAVKPDLAAITDFLYDRL
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHH
RGYLKDKGYSTNEVEAVVSQRPQRLDDIVARLEAVRAFAALPQAEALAAANKRITNILKK
HHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TDITIGSVQPQLLREDAERALHQAVATSEPHVHDAFARGDFTTALKTLASLREAVDSFFD
HCCEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GVMVMADDTALRDNRLALLGELHGLMNRVADISKLAA
CEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLLIELLTEELPPKALARLGEAFAQSLFDGLSAQGLLEEGAQVEGFATPRRLAASITG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHH
VRRAAPDRELREKVLPVNIAFDAEGKPTAPLTKKLAALAKSIGADTIAPESLERAPDGKA
HHHCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHH
ESLFHRYTARGAVLADGLQAALSQTIAGLPIPKVMIYQRPNGDNVQFVRPAHRLIALLDD
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEECHHHHHHHHHCC
EIIPAGVLGLQSGNVTLGHRFLSAGEIIIPHATAYASTLKSQGKVIAGYAERKEAIRAEL
CCCCCHHEEECCCCEEHHHHHHCCCCEEECCHHHHHHHHHHCCCEEECHHHHHHHHHHHH
LKAAGADTVVMPEALLDEVNALVEWPVVYPCHFEEQFLAVPQECLILTMQTNQKYFALTD
HHHCCCCCEECCHHHHHHHHHHHHCCEEECCCCCHHHHHCCHHHEEEEEECCCEEEEEEC
AQGHLRNRFLIVSNLATETPQAIIEGNERVVRPRLADARFFFEHDKKKPLADRVPQLARV
CCHHHHCCEEEEECCCCCCCHHHHCCCCCEECCCHHHHHHHHHCCCCCCHHHHHHHHHHH
VYHNKIGTQLERVSRLQAIAGQLAEKLGAEVAHASRAALLAKADLLTDMVGEFPELQGTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCH
GTYYARHDGEAEDVALACSEHYQPRFAGDALPGTATGTVVALADKLETLVGIWGIGLAPT
HHEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GEKDPFALRRHALGILRMLIEKPLALGIAEVLEAAAASFEGIAAVKPDLAAITDFLYDRL
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHH
RGYLKDKGYSTNEVEAVVSQRPQRLDDIVARLEAVRAFAALPQAEALAAANKRITNILKK
HHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
TDITIGSVQPQLLREDAERALHQAVATSEPHVHDAFARGDFTTALKTLASLREAVDSFFD
HCCEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GVMVMADDTALRDNRLALLGELHGLMNRVADISKLAA
CEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852