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The map label for this gene is thiF [H]

Identifier: 17545070

GI number: 17545070

Start: 382722

End: 383471

Strand: Reverse

Name: thiF [H]

Synonym: RSc0351

Alternate gene names: 17545070

Gene position: 383471-382722 (Counterclockwise)

Preceding gene: 17545071

Following gene: 17545059

Centisome position: 10.32

GC content: 70.27

Gene sequence:

>750_bases
ATGAACGACAACCAACTGCTGCGCTACTCCCGCCATATCCTGCTCGACGAGATCGGCATCGAGGGACAATCGCGGCTGCT
CGCCGCGCATGCGCTGGTGATCGGCGCCGGCGGACTGGGCGCGGCGGCCCTGCCCTACCTGGCCGCGGCAGGCATCGGCA
CCCTCACGATCGTCGATGACGACAGCGTCGACCTGACCAACCTGCAGCGCCAGATCATCCACACCACCGAGTCGGTCGGC
CACCCGAAGGTCGAATCGGCGCGGCAGGCCATTGCGCGGCTGAACCCGGAGGTGCGCGTCCACGCGGTGCGGCAACGGCT
CGATGCGGACGGCATCGGCGCGCTGCTGGACGGCGTGACGGTGGTGCTGGACTGTTCCGACAACTTCGCTACCCGCCAGG
CCACGAACCAGGCGTGCGTGCGTGCGCGGGTGCCGCTGGTATCCGGCGCGGCCATCCGCTTCGATGGGCAGATCAGCGTG
TTCGACAGCCGCACCGGCGGCCCCTGCTACGCCTGCCTGTTCCCGCCCGACGAACCCGCACCGGAGGCCGCCTGCGCGAC
CATGGGCGTGTTCTCGCCGCTGGTCGGCATGGTCGGGACGGTGCAGGCAGCGGAGGCGCTCAAGCTGGCAGCGGGCATCG
GGCGATCGCTCGCCGGACGCCTGCTGATGCTCGACGGCCTGGCCATGGAATGGACGACAATGCGCATCGCGCGGCAACCG
TCCTGCCCCGTCTGCGGCAGCGGCCACTGA

Upstream 100 bases:

>100_bases
CCGGCACCGGAACCCACCGGCGCACGATAAGCCCGCATCCGCCAGGACTGGCAACTGGCAAGGCCGTGCACCGCACGGCC
TTTTTCATTCCCGACGCGCC

Downstream 100 bases:

>100_bases
CCGGTCACCGGCGCAAAAGAAAAGGAGGCCGTAGCCTCCTTTCTTGTTTGCGCGCCGGGCAGCGTTACTCGCCTTCCGGC
TCCATCTGCGACTGCAGGTA

Product: molybdopterin biosynthesis protein MoeB

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNDNQLLRYSRHILLDEIGIEGQSRLLAAHALVIGAGGLGAAALPYLAAAGIGTLTIVDDDSVDLTNLQRQIIHTTESVG
HPKVESARQAIARLNPEVRVHAVRQRLDADGIGALLDGVTVVLDCSDNFATRQATNQACVRARVPLVSGAAIRFDGQISV
FDSRTGGPCYACLFPPDEPAPEAACATMGVFSPLVGMVGTVQAAEALKLAAGIGRSLAGRLLMLDGLAMEWTTMRIARQP
SCPVCGSGH

Sequences:

>Translated_249_residues
MNDNQLLRYSRHILLDEIGIEGQSRLLAAHALVIGAGGLGAAALPYLAAAGIGTLTIVDDDSVDLTNLQRQIIHTTESVG
HPKVESARQAIARLNPEVRVHAVRQRLDADGIGALLDGVTVVLDCSDNFATRQATNQACVRARVPLVSGAAIRFDGQISV
FDSRTGGPCYACLFPPDEPAPEAACATMGVFSPLVGMVGTVQAAEALKLAAGIGRSLAGRLLMLDGLAMEWTTMRIARQP
SCPVCGSGH
>Mature_249_residues
MNDNQLLRYSRHILLDEIGIEGQSRLLAAHALVIGAGGLGAAALPYLAAAGIGTLTIVDDDSVDLTNLQRQIIHTTESVG
HPKVESARQAIARLNPEVRVHAVRQRLDADGIGALLDGVTVVLDCSDNFATRQATNQACVRARVPLVSGAAIRFDGQISV
FDSRTGGPCYACLFPPDEPAPEAACATMGVFSPLVGMVGTVQAAEALKLAAGIGRSLAGRLLMLDGLAMEWTTMRIARQP
SCPVCGSGH

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=228, Percent_Identity=45.1754385964912, Blast_Score=170, Evalue=9e-43,
Organism=Homo sapiens, GI4885649, Length=146, Percent_Identity=28.0821917808219, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI38045948, Length=151, Percent_Identity=31.7880794701987, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787048, Length=249, Percent_Identity=44.9799196787149, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=46.1847389558233, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI1789177, Length=138, Percent_Identity=36.9565217391304, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17540406, Length=259, Percent_Identity=41.3127413127413, Blast_Score=208, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI193203301, Length=198, Percent_Identity=28.2828282828283, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI86565433, Length=171, Percent_Identity=27.4853801169591, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI86565431, Length=171, Percent_Identity=27.4853801169591, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=248, Percent_Identity=37.9032258064516, Blast_Score=175, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6320598, Length=157, Percent_Identity=26.1146496815287, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322639, Length=188, Percent_Identity=25.531914893617, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582879, Length=250, Percent_Identity=41.2, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24660640, Length=151, Percent_Identity=31.7880794701987, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573937, Length=185, Percent_Identity=28.6486486486486, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 26073; Mature: 26073

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNQLLRYSRHILLDEIGIEGQSRLLAAHALVIGAGGLGAAALPYLAAAGIGTLTIVDD
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCEEEEEEC
DSVDLTNLQRQIIHTTESVGHPKVESARQAIARLNPEVRVHAVRQRLDADGIGALLDGVT
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCEE
VVLDCSDNFATRQATNQACVRARVPLVSGAAIRFDGQISVFDSRTGGPCYACLFPPDEPA
EEEECCCCCHHHHHHHHHHHHHCCCEECCCEEEECCEEEEEECCCCCCEEEEECCCCCCC
PEAACATMGVFSPLVGMVGTVQAAEALKLAAGIGRSLAGRLLMLDGLAMEWTTMRIARQP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEECCCHHHHHHHHHHCCC
SCPVCGSGH
CCCCCCCCC
>Mature Secondary Structure
MNDNQLLRYSRHILLDEIGIEGQSRLLAAHALVIGAGGLGAAALPYLAAAGIGTLTIVDD
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCEEEEEEC
DSVDLTNLQRQIIHTTESVGHPKVESARQAIARLNPEVRVHAVRQRLDADGIGALLDGVT
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHCCEE
VVLDCSDNFATRQATNQACVRARVPLVSGAAIRFDGQISVFDSRTGGPCYACLFPPDEPA
EEEECCCCCHHHHHHHHHHHHHCCCEECCCEEEECCEEEEEECCCCCCEEEEECCCCCCC
PEAACATMGVFSPLVGMVGTVQAAEALKLAAGIGRSLAGRLLMLDGLAMEWTTMRIARQP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEECCCHHHHHHHHHHCCC
SCPVCGSGH
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]