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The map label for this gene is exoA [H]

Identifier: 17544859

GI number: 17544859

Start: 162230

End: 163015

Strand: Direct

Name: exoA [H]

Synonym: RSc0140

Alternate gene names: 17544859

Gene position: 162230-163015 (Clockwise)

Preceding gene: 17544857

Following gene: 17544860

Centisome position: 4.37

GC content: 64.63

Gene sequence:

>786_bases
ATGTTACGCATCATTTCCGCCAACCTCAACGGCGTCCGCTCCGCCGCCAGCAAGGGCTTTTTCGAATGGATGGGCAAGCA
GGACGCCGACTTCGTCTGCGTGCAGGAACTGAAATGCGCGCAGGACGACATGACCCCGGAATTCCTGGCGCCGCACGGCT
ACCACGGCGTGTTCCAGCATGCCGTCAAGAAGGGCTATAGCGGCGCAGGCCTGTACGCGCGGCATCAGCCGGATGACGTG
ATCGTCGGCTTCGACGGCGGCGAGTTCGATGCCGAGGGCCGCTACGTCGAGGCGCGCTACGGCAAGCTGTCGATCATCTC
GGTCTACGTGCCGTCCGGCTCCAGCGGCGAGGAGCGCCAGCAGGCCAAGTACCGCTTCATGGACCTGTTCATGCAGCACC
TGACCGACCTGCGCCACGAGAAGGGCCGCGAGGTCGTCCTGTGCGGCGACGTCAACATCGTCCACAAGGAAATCGACATC
AAGAACTGGAAGGGCAACCAGAAGAACTCCGGCTGCCTGCCCGAGGAGCGCGCATGGCTGACCCGGCTGTTCGACGAGGT
CGGCTACGTGGATGTCTTCCGCACGCTGGACCCGCGCCCCGAGCAGTACACCTGGTGGAGCAACCGCGGCCAGGCCTACG
CAAAGAACGTCGGGTGGCGCATCGACTACCAGATCGCCACGCCGGGCATCGCCGCCACGGCGCGCAGCACGTCGATCTTC
CGGGACATCAAGTTCAGCGACCATGCCCCGCTGACGGTCGACTACGACGCCAAGCTGCACGGGTAA

Upstream 100 bases:

>100_bases
GAACGACAGTACGCCGGCTTCCACCGCGAAGCGGATGAAGGATTGGCGCAACTCGCTGTTTTGGCTCATCTTTTTTCTCT
GGTCTGCAAAAAGTCCCGCC

Downstream 100 bases:

>100_bases
GCAGCACTGCCGCGCGGCTCCGATCCGCCCGGCGGCGATCGGGGCGTTGCTGGCCGTGTGCGTGAAGCTTAACCATGCGG
CGCCGGGCGGATGGTAAATT

Product: exodeoxyribonuclease III protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLRIISANLNGVRSAASKGFFEWMGKQDADFVCVQELKCAQDDMTPEFLAPHGYHGVFQHAVKKGYSGAGLYARHQPDDV
IVGFDGGEFDAEGRYVEARYGKLSIISVYVPSGSSGEERQQAKYRFMDLFMQHLTDLRHEKGREVVLCGDVNIVHKEIDI
KNWKGNQKNSGCLPEERAWLTRLFDEVGYVDVFRTLDPRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIAATARSTSIF
RDIKFSDHAPLTVDYDAKLHG

Sequences:

>Translated_261_residues
MLRIISANLNGVRSAASKGFFEWMGKQDADFVCVQELKCAQDDMTPEFLAPHGYHGVFQHAVKKGYSGAGLYARHQPDDV
IVGFDGGEFDAEGRYVEARYGKLSIISVYVPSGSSGEERQQAKYRFMDLFMQHLTDLRHEKGREVVLCGDVNIVHKEIDI
KNWKGNQKNSGCLPEERAWLTRLFDEVGYVDVFRTLDPRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIAATARSTSIF
RDIKFSDHAPLTVDYDAKLHG
>Mature_261_residues
MLRIISANLNGVRSAASKGFFEWMGKQDADFVCVQELKCAQDDMTPEFLAPHGYHGVFQHAVKKGYSGAGLYARHQPDDV
IVGFDGGEFDAEGRYVEARYGKLSIISVYVPSGSSGEERQQAKYRFMDLFMQHLTDLRHEKGREVVLCGDVNIVHKEIDI
KNWKGNQKNSGCLPEERAWLTRLFDEVGYVDVFRTLDPRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIAATARSTSIF
RDIKFSDHAPLTVDYDAKLHG

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=255, Percent_Identity=35.2941176470588, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI18375503, Length=255, Percent_Identity=35.2941176470588, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI18375501, Length=255, Percent_Identity=35.2941176470588, Blast_Score=162, Evalue=3e-40,
Organism=Escherichia coli, GI1788046, Length=269, Percent_Identity=32.3420074349442, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71989536, Length=259, Percent_Identity=29.3436293436293, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI221330655, Length=253, Percent_Identity=35.1778656126482, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI17136678, Length=253, Percent_Identity=35.1778656126482, Blast_Score=147, Evalue=6e-36,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29607; Mature: 29607

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIISANLNGVRSAASKGFFEWMGKQDADFVCVQELKCAQDDMTPEFLAPHGYHGVFQH
CEEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHH
AVKKGYSGAGLYARHQPDDVIVGFDGGEFDAEGRYVEARYGKLSIISVYVPSGSSGEERQ
HHHCCCCCCCEEEECCCCCEEEECCCCCCCCCCCEEEEECCEEEEEEEEECCCCCCHHHH
QAKYRFMDLFMQHLTDLRHEKGREVVLCGDVNIVHKEIDIKNWKGNQKNSGCLPEERAWL
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCCCCCCCCCCCHHHHHH
TRLFDEVGYVDVFRTLDPRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIAATARSTSIF
HHHHHHCCHHHHHHHCCCCCCCEEEECCCCCEEECCCCEEEEEEEECCCCEEHHHCHHHH
RDIKFSDHAPLTVDYDAKLHG
EECCCCCCCCEEEECCCEECC
>Mature Secondary Structure
MLRIISANLNGVRSAASKGFFEWMGKQDADFVCVQELKCAQDDMTPEFLAPHGYHGVFQH
CEEEEECCCCHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHH
AVKKGYSGAGLYARHQPDDVIVGFDGGEFDAEGRYVEARYGKLSIISVYVPSGSSGEERQ
HHHCCCCCCCEEEECCCCCEEEECCCCCCCCCCCEEEEECCEEEEEEEEECCCCCCHHHH
QAKYRFMDLFMQHLTDLRHEKGREVVLCGDVNIVHKEIDIKNWKGNQKNSGCLPEERAWL
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCCCCCCCCCCCHHHHHH
TRLFDEVGYVDVFRTLDPRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIAATARSTSIF
HHHHHHCCHHHHHHHCCCCCCCEEEECCCCCEEECCCCEEEEEEEECCCCEEHHHCHHHH
RDIKFSDHAPLTVDYDAKLHG
EECCCCCCCCEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]