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The map label for this gene is pyrE [H]

Identifier: 17544858

GI number: 17544858

Start: 161518

End: 162231

Strand: Reverse

Name: pyrE [H]

Synonym: RSc0139

Alternate gene names: 17544858

Gene position: 162231-161518 (Counterclockwise)

Preceding gene: 17544863

Following gene: 17544853

Centisome position: 4.37

GC content: 64.99

Gene sequence:

>714_bases
ATGGCGGGACTTTTTGCAGACCAGAGAAAAAAGATGAGCCAAAACAGCGAGTTGCGCCAATCCTTCATCCGCTTCGCGGT
GGAAGCCGGCGTACTGTCGTTCGGGGAATTCGTGACCAAGGCGGGGCGGACGTCGCCCTATTTCTTCAACGCCGGCAAGT
TCAGCGACGGCGCGCTGCTCGGCCAGGTCGCGCAATTCTATGCGAAAACCCTGCTGGACTCGGGCGTGCAGTTCGACATG
CTGTTCGGCCCCGCCTACAAGGGCATCACGCTCGCTTCGGCCACCGCCGTGGCGCTGGCCGGCATGGGCCGCAACGTAGG
CTTCGCCTACAACCGCAAGGAAGCCAAGGACCACGGCGAAGGCGGCAGCCTGGTCGGCGCCAAGCTGCAGGGCCGCGTGG
TGATCGTCGATGACGTGATCTCCGCCGGCACCTCGGTGCGCGAATCGGTGGAGCTGATCCGCAACGCGGGCGCCACCCCC
GCCGCCGTGCTGATCCTGATGGACCGCATGGAGCGCAGCGGCAACGCCGTCGACATCGGCGAACGCTCGGCGGTGCAGGA
CGTGCAGGCGCAATACGGCATGCCGGTGGTGTCGATTGCCAACCTGGATGATCTGCTGGGGTATCTCGACCATGCCGGCG
ATCCGGCGCTGGCGGGTTATCGTGCCAAGGCGGCGGCGTATCGGGACAAGTACGGCGTCAGCGCTGTTGTTTGA

Upstream 100 bases:

>100_bases
CACGCAGACGAAGTCGGCGTCCTGCTTGCCCATCCATTCGAAAAAGCCCTTGCTGGCGGCGGAGCGGACGCCGTTGAGGT
TGGCGGAAATGATGCGTAAC

Downstream 100 bases:

>100_bases
TTGGTTTTGCGTTTTGGGCTGAGTGCTTTTGGCGCTTGCTTTGGGGGTGGAGCTTGGCGGCTCCCCCGATTTCGTCCCCC
GCCAGGGCCGGGACGAACGC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MAGLFADQRKKMSQNSELRQSFIRFAVEAGVLSFGEFVTKAGRTSPYFFNAGKFSDGALLGQVAQFYAKTLLDSGVQFDM
LFGPAYKGITLASATAVALAGMGRNVGFAYNRKEAKDHGEGGSLVGAKLQGRVVIVDDVISAGTSVRESVELIRNAGATP
AAVLILMDRMERSGNAVDIGERSAVQDVQAQYGMPVVSIANLDDLLGYLDHAGDPALAGYRAKAAAYRDKYGVSAVV

Sequences:

>Translated_237_residues
MAGLFADQRKKMSQNSELRQSFIRFAVEAGVLSFGEFVTKAGRTSPYFFNAGKFSDGALLGQVAQFYAKTLLDSGVQFDM
LFGPAYKGITLASATAVALAGMGRNVGFAYNRKEAKDHGEGGSLVGAKLQGRVVIVDDVISAGTSVRESVELIRNAGATP
AAVLILMDRMERSGNAVDIGERSAVQDVQAQYGMPVVSIANLDDLLGYLDHAGDPALAGYRAKAAAYRDKYGVSAVV
>Mature_236_residues
AGLFADQRKKMSQNSELRQSFIRFAVEAGVLSFGEFVTKAGRTSPYFFNAGKFSDGALLGQVAQFYAKTLLDSGVQFDML
FGPAYKGITLASATAVALAGMGRNVGFAYNRKEAKDHGEGGSLVGAKLQGRVVIVDDVISAGTSVRESVELIRNAGATPA
AVLILMDRMERSGNAVDIGERSAVQDVQAQYGMPVVSIANLDDLLGYLDHAGDPALAGYRAKAAAYRDKYGVSAVV

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=180, Percent_Identity=28.8888888888889, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1790073, Length=216, Percent_Identity=53.7037037037037, Blast_Score=227, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17508631, Length=139, Percent_Identity=30.2158273381295, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554964, Length=203, Percent_Identity=25.1231527093596, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323530, Length=222, Percent_Identity=43.2432432432432, Blast_Score=178, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6323927, Length=222, Percent_Identity=38.7387387387387, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 25074; Mature: 24943

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLFADQRKKMSQNSELRQSFIRFAVEAGVLSFGEFVTKAGRTSPYFFNAGKFSDGALL
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHH
GQVAQFYAKTLLDSGVQFDMLFGPAYKGITLASATAVALAGMGRNVGFAYNRKEAKDHGE
HHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHCCCCCCCEECCHHHHCCCC
GGSLVGAKLQGRVVIVDDVISAGTSVRESVELIRNAGATPAAVLILMDRMERSGNAVDIG
CCCEEEEEECCCEEEEECHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEECCC
ERSAVQDVQAQYGMPVVSIANLDDLLGYLDHAGDPALAGYRAKAAAYRDKYGVSAVV
CHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCHHCHHHHHHHHHHHCCCEECC
>Mature Secondary Structure 
AGLFADQRKKMSQNSELRQSFIRFAVEAGVLSFGEFVTKAGRTSPYFFNAGKFSDGALL
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHH
GQVAQFYAKTLLDSGVQFDMLFGPAYKGITLASATAVALAGMGRNVGFAYNRKEAKDHGE
HHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHCCCCCCCEECCHHHHCCCC
GGSLVGAKLQGRVVIVDDVISAGTSVRESVELIRNAGATPAAVLILMDRMERSGNAVDIG
CCCEEEEEECCCEEEEECHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEECCC
ERSAVQDVQAQYGMPVVSIANLDDLLGYLDHAGDPALAGYRAKAAAYRDKYGVSAVV
CHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCHHCHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]