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The map label for this gene is metK [H]

Identifier: 17544853

GI number: 17544853

Start: 156370

End: 157560

Strand: Reverse

Name: metK [H]

Synonym: RSc0134

Alternate gene names: 17544853

Gene position: 157560-156370 (Counterclockwise)

Preceding gene: 17544858

Following gene: 17544851

Centisome position: 4.24

GC content: 68.26

Gene sequence:

>1191_bases
GTGTCAAACGACTTCCTCTTCACCTCGGAGTCGGTCTCCGAGGGCCATCCGGACAAGGTCGCCGACCAGATTTCCGACGC
CATCCTGGACGCGATCCTCGCCCAGGACAAATATGCGCGCGTCGCCGCGGAAACGCTGTGCAACACCGGCCTGGTGGTGC
TGGCCGGCGAGATCACCACGTCCGCCAACGTCGACTACATCCACGTCGCGCGCGAGACCATCAAGCGCATCGGCTACGAC
AACACCGACTACGGCATCGACTACAAGGGCTGCGCGGTGCTGGTCGCCTACGACAAGCAGTCGCCCGACATCGCCCAGGG
CGTGGACCGCGCGTCCGACGACTACCTGAACCAGGGCGCCGGCGACCAGGGCCTGATGTTCGGCTACGCCTGCGACGAAA
CGCCCGAGCTGATGCCCTTCCCGATCTACTACGCGCACCGCCTGGTCGAGCGCCAGTCGCAGCTGCGCCGCGACGGCCGC
CTGCCCTGGCTGCGCCCCGACGCGAAGTCGCAGGTGACGGTGCGCTACGTGAACGGCAAGCCGCACAGCGTCGACACCGT
GGTGCTGTCCACCCAGCACGCGCCGGAGATCTCGCAGGAGCAGATCCGCGAGGCCGTGATCGAAGAGATCATCAAGCCGG
TGCTGCCCTCGCACATGCTGGCCGAGACCAAGTACCTGGTGAACCCGACCGGCCGCTTCGTCATCGGCGGCCCGCAGGGC
GACTGCGGCCTGACCGGCCGCAAGATCATCGTCGACACCTATGGCGGCGCGGCCCCGCACGGCGGCGGCGCGTTCTCGGG
CAAGGACCCGTCCAAGGTGGACCGCTCGGCCGCCTACGCCGCGCGCTACGTGGCCAAGAACGTGGTGGCGGCGGGCCTGG
CGCGCCAGTGCCAGGTGCAGGTGAGCTACGCCATCGGCGTGGCGCGTCCGATCAACATCACGGTGTACACCGAAGGCACC
GGCGTGATCCCGGACGAGCAGATCGCCAAGCTCGTGCAGGAGCACTTCGACCTGCGTCCGAAGGGCATCGTGCAGATGCT
GGACCTGCTGCGCCCGATCTACGGCAAGACCGCCGCCTACGGCCACTTCGGCCGCGAAGAGCCGGAGTTCAGCTGGGAAG
CCACCGACAAGGCCCAACTGCTGCGCGAGGCAGCCGGGCTGGCCGGCGAGCCGGTCAAGGCGTTCGCGTAA

Upstream 100 bases:

>100_bases
CGGCCACAAACCTGGATGAGGTTGATAAATACCGCTAAAGCGTCGCCGGCCGTGGCACAAACGGATTGGCAACGTGATAG
CCGATCTGGAGCTAACAGCC

Downstream 100 bases:

>100_bases
GCACGGCGCCGCCGCACGGAAGAAACCGCCCACCGGGGCGGTTTCTTTTTATGTGCCGCGCGAATCCGCACATCAAGGCA
TCTTGCTGAGGATCTGGTTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSNDFLFTSESVSEGHPDKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITTSANVDYIHVARETIKRIGYD
NTDYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYAHRLVERQSQLRRDGR
LPWLRPDAKSQVTVRYVNGKPHSVDTVVLSTQHAPEISQEQIREAVIEEIIKPVLPSHMLAETKYLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINITVYTEGT
GVIPDEQIAKLVQEHFDLRPKGIVQMLDLLRPIYGKTAAYGHFGREEPEFSWEATDKAQLLREAAGLAGEPVKAFA

Sequences:

>Translated_396_residues
MSNDFLFTSESVSEGHPDKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITTSANVDYIHVARETIKRIGYD
NTDYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYAHRLVERQSQLRRDGR
LPWLRPDAKSQVTVRYVNGKPHSVDTVVLSTQHAPEISQEQIREAVIEEIIKPVLPSHMLAETKYLVNPTGRFVIGGPQG
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINITVYTEGT
GVIPDEQIAKLVQEHFDLRPKGIVQMLDLLRPIYGKTAAYGHFGREEPEFSWEATDKAQLLREAAGLAGEPVKAFA
>Mature_395_residues
SNDFLFTSESVSEGHPDKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITTSANVDYIHVARETIKRIGYDN
TDYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGAGDQGLMFGYACDETPELMPFPIYYAHRLVERQSQLRRDGRL
PWLRPDAKSQVTVRYVNGKPHSVDTVVLSTQHAPEISQEQIREAVIEEIIKPVLPSHMLAETKYLVNPTGRFVIGGPQGD
CGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQVSYAIGVARPINITVYTEGTG
VIPDEQIAKLVQEHFDLRPKGIVQMLDLLRPIYGKTAAYGHFGREEPEFSWEATDKAQLLREAAGLAGEPVKAFA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=60, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=58.6387434554974, Blast_Score=422, Evalue=1e-118,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=70.2349869451697, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17538498, Length=376, Percent_Identity=57.9787234042553, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17538494, Length=393, Percent_Identity=56.234096692112, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25145633, Length=383, Percent_Identity=56.6579634464752, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17551082, Length=378, Percent_Identity=55.8201058201058, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=393, Percent_Identity=50.8905852417303, Blast_Score=357, Evalue=8e-99,
Organism=Caenorhabditis elegans, GI32565911, Length=330, Percent_Identity=55.4545454545455, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI25145635, Length=335, Percent_Identity=54.0298507462687, Blast_Score=348, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI32565903, Length=320, Percent_Identity=53.4375, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=61.9047619047619, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=81.5789473684211, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320710, Length=386, Percent_Identity=58.0310880829015, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=58.994708994709, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45552159, Length=376, Percent_Identity=56.1170212765958, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=376, Percent_Identity=56.1170212765958, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=376, Percent_Identity=56.1170212765958, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=376, Percent_Identity=56.1170212765958, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=376, Percent_Identity=56.1170212765958, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=376, Percent_Identity=56.1170212765958, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=376, Percent_Identity=55.5851063829787, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580525, Length=376, Percent_Identity=55.5851063829787, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580541, Length=377, Percent_Identity=53.0503978779841, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580539, Length=121, Percent_Identity=58.6776859504132, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43102; Mature: 42971

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDFLFTSESVSEGHPDKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITT
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEC
SANVDYIHVARETIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGA
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHCCC
GDQGLMFGYACDETPELMPFPIYYAHRLVERQSQLRRDGRLPWLRPDAKSQVTVRYVNGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSVDTVVLSTQHAPEISQEQIREAVIEEIIKPVLPSHMLAETKYLVNPTGRFVIGGPQG
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCEEECCCCCEEEECCCC
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VSYAIGVARPINITVYTEGTGVIPDEQIAKLVQEHFDLRPKGIVQMLDLLRPIYGKTAAY
EEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHC
GHFGREEPEFSWEATDKAQLLREAAGLAGEPVKAFA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCC
>Mature Secondary Structure 
SNDFLFTSESVSEGHPDKVADQISDAILDAILAQDKYARVAAETLCNTGLVVLAGEITT
CCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEC
SANVDYIHVARETIKRIGYDNTDYGIDYKGCAVLVAYDKQSPDIAQGVDRASDDYLNQGA
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCHHHHHCCC
GDQGLMFGYACDETPELMPFPIYYAHRLVERQSQLRRDGRLPWLRPDAKSQVTVRYVNGK
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PHSVDTVVLSTQHAPEISQEQIREAVIEEIIKPVLPSHMLAETKYLVNPTGRFVIGGPQG
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCEEECCCCCEEEECCCC
DCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLARQCQVQ
CCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
VSYAIGVARPINITVYTEGTGVIPDEQIAKLVQEHFDLRPKGIVQMLDLLRPIYGKTAAY
EEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHC
GHFGREEPEFSWEATDKAQLLREAAGLAGEPVKAFA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA