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The map label for this gene is thiE1 [H]

Identifier: 17544827

GI number: 17544827

Start: 125843

End: 126994

Strand: Reverse

Name: thiE1 [H]

Synonym: RSc0108

Alternate gene names: 17544827

Gene position: 126994-125843 (Counterclockwise)

Preceding gene: 17544828

Following gene: 17544826

Centisome position: 3.42

GC content: 75.26

Gene sequence:

>1152_bases
ATGAGCGCATCCGCCCTGCGCTTTGCCAGCGCCTGGCCGGATGCCGCCGCGCTGGCCGACCGCCTCATCGATCGCCACGC
CGATGCCTTCGGCCGCGCGCCGCACGCCTGGAGCGTGACCGACCGCGCCGATGACGCCGCGACCGCCGCCGTCCTGCTGA
CCACCGACGCCGCGCAGGCCGAACGCGCCCGCGCCGCCGGCGCCGCCGTCGTGCTGAGCGAGGCGCGCAACGGCGAGCGC
ATCGACACCGTGCACGACCGCCTCGGCACCTACCGCTTCGCCGCCCCGGCGACGGGCGCGGTGTTCGGCGAACGCTTCGT
CGCCATGTTCGGCGCCGCGCTGGCGCTGGCCTTCGAGCCGCGCGACGCGCTGTGCGTGGCGCGTGCGTGGATCGCGGAGG
CCGCCGCCGATGCGCTGGCGTGGCCCGCCCGCTTCGACGCGCTGCCCCGCGTACTCGAACCTGCGCTGCCGTGCGCGGCC
TCGCCGGACCTCGCCTTCGCGCCCTGCCCGCCGCGGCTCGGCGTGTATGCCGTGGTGCCGGACGCGGAATGGGTCGAGCG
CCTCGTTGCGCTCAAGGTGCCGACCGTGCAATTGCGCGTCAAATCGGACGACGCGCGGGCCGTCGCCGGGCAGGTGCGCC
GCGCCGCCGCTGCCGCGCGCGGCAGCCAGACGCGCCTGTTCATCAACGACCACTGGCGCGTTGCACTGGACGTGCATGCG
CAGACGCCCGACAGCGGCCTCTATGGCATCCACCTCGGCCAGGAAGATATCGACGATGCGGATCTCGCGGCCATCCGCGC
GTCCGGCCTGCGCCTGGGCATCAGCACGCACGGCTACGCTGAGATGCTGCGCGTCGCTGCGCTGAACCCGAGCTACCTCG
CGCTGGGCGCGGTCTTCGCCACGCCCACCAAGACCATGCCGACCCTGCCGCAGGGTCTGGGCCGGCTGTTCGCCCATGCG
GCGGCGATGCGCTCGCGCGTGCCGGCGCCGCCGCTCGTCGCCATCGGCGGCATCGACCTGGCTGCGATGCCGCGCGTGCT
CGAGAGCGGCGTCGGCGGCGTGGCCGTGGTGCGCGCCATCACCCAGGCGGAGGATGTGCCCGCCGCCGTGCAGGCGCTGC
AGGCGACGTTCGCGGCGCACGCGCGCGCCTGA

Upstream 100 bases:

>100_bases
GCCTGGCGCGGCTGTCGGGCCCGATGCCGGAGCGCGATGTCGCGCAGGCGAGCACGCCGGTGGTCGGCCTGCCGTTCTGG
CATGCCGACAAGAACAGCGC

Downstream 100 bases:

>100_bases
GCACCGCCCGCGCCACGCCCCCCCGGCCCCATCGCGCAACGGTCATCCGCCTGACCTATCCTGTTGCATGCGCCGCCGCA
GCGGTCGTTGCGGCGACGCA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSASALRFASAWPDAAALADRLIDRHADAFGRAPHAWSVTDRADDAATAAVLLTTDAAQAERARAAGAAVVLSEARNGER
IDTVHDRLGTYRFAAPATGAVFGERFVAMFGAALALAFEPRDALCVARAWIAEAAADALAWPARFDALPRVLEPALPCAA
SPDLAFAPCPPRLGVYAVVPDAEWVERLVALKVPTVQLRVKSDDARAVAGQVRRAAAAARGSQTRLFINDHWRVALDVHA
QTPDSGLYGIHLGQEDIDDADLAAIRASGLRLGISTHGYAEMLRVAALNPSYLALGAVFATPTKTMPTLPQGLGRLFAHA
AAMRSRVPAPPLVAIGGIDLAAMPRVLESGVGGVAVVRAITQAEDVPAAVQALQATFAAHARA

Sequences:

>Translated_383_residues
MSASALRFASAWPDAAALADRLIDRHADAFGRAPHAWSVTDRADDAATAAVLLTTDAAQAERARAAGAAVVLSEARNGER
IDTVHDRLGTYRFAAPATGAVFGERFVAMFGAALALAFEPRDALCVARAWIAEAAADALAWPARFDALPRVLEPALPCAA
SPDLAFAPCPPRLGVYAVVPDAEWVERLVALKVPTVQLRVKSDDARAVAGQVRRAAAAARGSQTRLFINDHWRVALDVHA
QTPDSGLYGIHLGQEDIDDADLAAIRASGLRLGISTHGYAEMLRVAALNPSYLALGAVFATPTKTMPTLPQGLGRLFAHA
AAMRSRVPAPPLVAIGGIDLAAMPRVLESGVGGVAVVRAITQAEDVPAAVQALQATFAAHARA
>Mature_382_residues
SASALRFASAWPDAAALADRLIDRHADAFGRAPHAWSVTDRADDAATAAVLLTTDAAQAERARAAGAAVVLSEARNGERI
DTVHDRLGTYRFAAPATGAVFGERFVAMFGAALALAFEPRDALCVARAWIAEAAADALAWPARFDALPRVLEPALPCAAS
PDLAFAPCPPRLGVYAVVPDAEWVERLVALKVPTVQLRVKSDDARAVAGQVRRAAAAARGSQTRLFINDHWRVALDVHAQ
TPDSGLYGIHLGQEDIDDADLAAIRASGLRLGISTHGYAEMLRVAALNPSYLALGAVFATPTKTMPTLPQGLGRLFAHAA
AMRSRVPAPPLVAIGGIDLAAMPRVLESGVGGVAVVRAITQAEDVPAAVQALQATFAAHARA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=205, Percent_Identity=49.7560975609756, Blast_Score=171, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 40037; Mature: 39906

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASALRFASAWPDAAALADRLIDRHADAFGRAPHAWSVTDRADDAATAAVLLTTDAAQA
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEEEEEECCHHHH
ERARAAGAAVVLSEARNGERIDTVHDRLGTYRFAAPATGAVFGERFVAMFGAALALAFEP
HHHHHCCHHEEEECCCCCCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHEEECC
RDALCVARAWIAEAAADALAWPARFDALPRVLEPALPCAASPDLAFAPCPPRLGVYAVVP
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCEEEECC
DAEWVERLVALKVPTVQLRVKSDDARAVAGQVRRAAAAARGSQTRLFINDHWRVALDVHA
CHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEE
QTPDSGLYGIHLGQEDIDDADLAAIRASGLRLGISTHGYAEMLRVAALNPSYLALGAVFA
CCCCCCEEEEECCCCCCCCHHHHHHHHCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHC
TPTKTMPTLPQGLGRLFAHAAAMRSRVPAPPLVAIGGIDLAAMPRVLESGVGGVAVVRAI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHH
TQAEDVPAAVQALQATFAAHARA
HHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SASALRFASAWPDAAALADRLIDRHADAFGRAPHAWSVTDRADDAATAAVLLTTDAAQA
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEEEEEECCHHHH
ERARAAGAAVVLSEARNGERIDTVHDRLGTYRFAAPATGAVFGERFVAMFGAALALAFEP
HHHHHCCHHEEEECCCCCCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHEEECC
RDALCVARAWIAEAAADALAWPARFDALPRVLEPALPCAASPDLAFAPCPPRLGVYAVVP
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCEEEECC
DAEWVERLVALKVPTVQLRVKSDDARAVAGQVRRAAAAARGSQTRLFINDHWRVALDVHA
CHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEE
QTPDSGLYGIHLGQEDIDDADLAAIRASGLRLGISTHGYAEMLRVAALNPSYLALGAVFA
CCCCCCEEEEECCCCCCCCHHHHHHHHCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHC
TPTKTMPTLPQGLGRLFAHAAAMRSRVPAPPLVAIGGIDLAAMPRVLESGVGGVAVVRAI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHH
TQAEDVPAAVQALQATFAAHARA
HHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA