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The map label for this gene is thiG [H]

Identifier: 17544828

GI number: 17544828

Start: 126991

End: 127818

Strand: Reverse

Name: thiG [H]

Synonym: RSc0109

Alternate gene names: 17544828

Gene position: 127818-126991 (Counterclockwise)

Preceding gene: 17544830

Following gene: 17544827

Centisome position: 3.44

GC content: 71.86

Gene sequence:

>828_bases
ATGACCACTACGAATCTTTCCGCGCTGGCCGATCCGCTGCGGCTGTATGACGAAGTCTTCGGCTCGCGGCTGCTGCTGGG
CACCGCGCGCTATCCGTCGCCGCAATCGCTGGAAGACGCGGTGCGCGCGTGCGGCCCGGCCATGCTGACCGTGGCGCTGC
GCCGCCAGAGCGCCGGCACACCCGAAGGCGGCGCGGGCTTCTGGAAGATGCTGCGCGCGCTGGGCGTGCCCGTGCTGCCC
AACACGGCCGGCTGCTACTCCGCCGACGAGGCCTACACGCTCGCGCAGATGTCGCGCGAAGTGTTCGAGACCGACTGGAT
CAAGCTCGAAGTCATCGGCGACGACTACACGCTGCAGCCCGACACGCTAGCGCTGCCTGCGGCCGCCGAACGCCTGATCC
GCGACGGCTTCAAGGTGCTGCCGTACTGCACCGAAGACCTTGTGCTGTGCCGCCGCCTGCTCGACGTCGGCTGCCGGGCG
CTGATGCCGTGGGCCGCCCCCATCGGCACCGGGCGCGGGCCGACCAACCCGTACGGCCTGCGCCTGCTGCGCGAGCGCCT
GCCCGACGTGCCGCTGATCGTCGATGCCGGCCTGGGCGTGCCGTCGCACGCCACGCAGGTGATGGAGTGGGGCTACGACG
GCGTGCTGCTCAACACCGCCGTCGCGCAGGCGGGCGATCCGGTGGCGATGGCGCGCGCGTTCGCGATGGCCACGCAGGCC
GGCCGCCTGGCGCGGCTGTCGGGCCCGATGCCGGAGCGCGATGTCGCGCAGGCGAGCACGCCGGTGGTCGGCCTGCCGTT
CTGGCATGCCGACAAGAACAGCGCATGA

Upstream 100 bases:

>100_bases
GCCGTCCCATTGGCATGCTGGCATCTCCCGTTTCGTTGATGCCAACATCCTATGTAAACTTAGACCAGTTAACAACGGAA
CAGGACACTAGGAGTCCCTG

Downstream 100 bases:

>100_bases
GCGCATCCGCCCTGCGCTTTGCCAGCGCCTGGCCGGATGCCGCCGCGCTGGCCGACCGCCTCATCGATCGCCACGCCGAT
GCCTTCGGCCGCGCGCCGCA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTTTNLSALADPLRLYDEVFGSRLLLGTARYPSPQSLEDAVRACGPAMLTVALRRQSAGTPEGGAGFWKMLRALGVPVLP
NTAGCYSADEAYTLAQMSREVFETDWIKLEVIGDDYTLQPDTLALPAAAERLIRDGFKVLPYCTEDLVLCRRLLDVGCRA
LMPWAAPIGTGRGPTNPYGLRLLRERLPDVPLIVDAGLGVPSHATQVMEWGYDGVLLNTAVAQAGDPVAMARAFAMATQA
GRLARLSGPMPERDVAQASTPVVGLPFWHADKNSA

Sequences:

>Translated_275_residues
MTTTNLSALADPLRLYDEVFGSRLLLGTARYPSPQSLEDAVRACGPAMLTVALRRQSAGTPEGGAGFWKMLRALGVPVLP
NTAGCYSADEAYTLAQMSREVFETDWIKLEVIGDDYTLQPDTLALPAAAERLIRDGFKVLPYCTEDLVLCRRLLDVGCRA
LMPWAAPIGTGRGPTNPYGLRLLRERLPDVPLIVDAGLGVPSHATQVMEWGYDGVLLNTAVAQAGDPVAMARAFAMATQA
GRLARLSGPMPERDVAQASTPVVGLPFWHADKNSA
>Mature_274_residues
TTTNLSALADPLRLYDEVFGSRLLLGTARYPSPQSLEDAVRACGPAMLTVALRRQSAGTPEGGAGFWKMLRALGVPVLPN
TAGCYSADEAYTLAQMSREVFETDWIKLEVIGDDYTLQPDTLALPAAAERLIRDGFKVLPYCTEDLVLCRRLLDVGCRAL
MPWAAPIGTGRGPTNPYGLRLLRERLPDVPLIVDAGLGVPSHATQVMEWGYDGVLLNTAVAQAGDPVAMARAFAMATQAG
RLARLSGPMPERDVAQASTPVVGLPFWHADKNSA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=44.6640316205534, Blast_Score=211, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 29538; Mature: 29407

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTNLSALADPLRLYDEVFGSRLLLGTARYPSPQSLEDAVRACGPAMLTVALRRQSAGT
CCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCC
PEGGAGFWKMLRALGVPVLPNTAGCYSADEAYTLAQMSREVFETDWIKLEVIGDDYTLQP
CCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEECC
DTLALPAAAERLIRDGFKVLPYCTEDLVLCRRLLDVGCRALMPWAAPIGTGRGPTNPYGL
CCEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
RLLRERLPDVPLIVDAGLGVPSHATQVMEWGYDGVLLNTAVAQAGDPVAMARAFAMATQA
HHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHC
GRLARLSGPMPERDVAQASTPVVGLPFWHADKNSA
CCHHHCCCCCCCHHHHHCCCCEEECCEECCCCCCC
>Mature Secondary Structure 
TTTNLSALADPLRLYDEVFGSRLLLGTARYPSPQSLEDAVRACGPAMLTVALRRQSAGT
CCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCC
PEGGAGFWKMLRALGVPVLPNTAGCYSADEAYTLAQMSREVFETDWIKLEVIGDDYTLQP
CCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEECC
DTLALPAAAERLIRDGFKVLPYCTEDLVLCRRLLDVGCRALMPWAAPIGTGRGPTNPYGL
CCEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
RLLRERLPDVPLIVDAGLGVPSHATQVMEWGYDGVLLNTAVAQAGDPVAMARAFAMATQA
HHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHC
GRLARLSGPMPERDVAQASTPVVGLPFWHADKNSA
CCHHHCCCCCCCHHHHHCCCCEEECCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA