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The map label for this gene is sigF [H]

Identifier: 17231345

GI number: 17231345

Start: 4649618

End: 4650394

Strand: Reverse

Name: sigF [H]

Synonym: all3853

Alternate gene names: 17231345

Gene position: 4650394-4649618 (Counterclockwise)

Preceding gene: 17231346

Following gene: 17231344

Centisome position: 72.51

GC content: 42.6

Gene sequence:

>777_bases
ATGCCTACAACAGCTACCAACGAATTGAAGTATGAAATTTGGCAGTTGTTACGAGAATATCAGCAATCTCGATCAGAAAC
TGTTCGTAATCAACTAGTAAAACTGAATCTTGGATTAGTGAGGAAAGAAGCTCACTACTGGATGAATCAATGCCGGGAAA
ACTATGATGATTTGGTTCAAGTTGGTTGTTTAGGTTTAATCAGAGCGATTGAAAGATTTGAAATTGCCAAAGGCCATGCT
TTTAGCTCCTTTGCCATACCTTATATCCGTGGCGAAATTCAACACTACCTCCGCGATAAAGGTGTAACAGTAAGAATTCC
TCGTCGCTACTTGGCGATACAACAGCGAGCAATCGGAGTTTCGCGTTCTTTGCGGGAACAATACAACCGCCAACCAACTG
ACTCAGAATTAGCAACAGCACTGGAAATTTCCCCAGATGAATGGCAGGAAATCAAATTAGCGTGGGTAAACCGCGCTCCT
TTGAGTTTAGATGTACCCGTGCAAGATGGTGAAGAAGGAGCCACTAGCCTGGGAGAATTGGTTCCTGATCATCATTACCG
CAGTTTTCAGTTGGCACAAGAAGACCAAATTCGCTTACAACAAGCATTGTTTCAGTTAGAACAACGCACTCGTGAAGTTT
TAGAGTGTGTGTTTTTACAAGACTTAACGCAGAAACAGGTGGCAGAACACTTAGGAATCAGCGTCGTCACAGTTTCCAGG
AGAGTAAAAAAAGGGTTGGACTTGTTAAAGCATCTGATGTGTGTGGCAGATGATTAA

Upstream 100 bases:

>100_bases
CACAGAGCAAATAATTTGACATTTGCGTTGATAGTGCTTAATCGCAGCATTCTTTAAAAGCGATTACTCAAATAATTATT
TACTTAATAAATGCGGTGCT

Downstream 100 bases:

>100_bases
AGTTCCATTAAGTCATCAGCATTTATTCTGAATGCTTGGTGCCAAAAACAACTCTTTAAATCCCAAAAAATTAGGTTATA
AAGGAAACGACAACAATGCC

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPTTATNELKYEIWQLLREYQQSRSETVRNQLVKLNLGLVRKEAHYWMNQCRENYDDLVQVGCLGLIRAIERFEIAKGHA
FSSFAIPYIRGEIQHYLRDKGVTVRIPRRYLAIQQRAIGVSRSLREQYNRQPTDSELATALEISPDEWQEIKLAWVNRAP
LSLDVPVQDGEEGATSLGELVPDHHYRSFQLAQEDQIRLQQALFQLEQRTREVLECVFLQDLTQKQVAEHLGISVVTVSR
RVKKGLDLLKHLMCVADD

Sequences:

>Translated_258_residues
MPTTATNELKYEIWQLLREYQQSRSETVRNQLVKLNLGLVRKEAHYWMNQCRENYDDLVQVGCLGLIRAIERFEIAKGHA
FSSFAIPYIRGEIQHYLRDKGVTVRIPRRYLAIQQRAIGVSRSLREQYNRQPTDSELATALEISPDEWQEIKLAWVNRAP
LSLDVPVQDGEEGATSLGELVPDHHYRSFQLAQEDQIRLQQALFQLEQRTREVLECVFLQDLTQKQVAEHLGISVVTVSR
RVKKGLDLLKHLMCVADD
>Mature_257_residues
PTTATNELKYEIWQLLREYQQSRSETVRNQLVKLNLGLVRKEAHYWMNQCRENYDDLVQVGCLGLIRAIERFEIAKGHAF
SSFAIPYIRGEIQHYLRDKGVTVRIPRRYLAIQQRAIGVSRSLREQYNRQPTDSELATALEISPDEWQEIKLAWVNRAPL
SLDVPVQDGEEGATSLGELVPDHHYRSFQLAQEDQIRLQQALFQLEQRTREVLECVFLQDLTQKQVAEHLGISVVTVSRR
VKKGLDLLKHLMCVADD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. It is responsible for directing gene

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=237, Percent_Identity=26.1603375527426, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788231, Length=188, Percent_Identity=30.8510638297872, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789098, Length=232, Percent_Identity=25.8620689655172, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29940; Mature: 29808

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTATNELKYEIWQLLREYQQSRSETVRNQLVKLNLGLVRKEAHYWMNQCRENYDDLVQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGCLGLIRAIERFEIAKGHAFSSFAIPYIRGEIQHYLRDKGVTVRIPRRYLAIQQRAIGV
HHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHH
SRSLREQYNRQPTDSELATALEISPDEWQEIKLAWVNRAPLSLDVPVQDGEEGATSLGEL
HHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHEECCCCCEEECCCCCCCHHHHHHHHH
VPDHHYRSFQLAQEDQIRLQQALFQLEQRTREVLECVFLQDLTQKQVAEHLGISVVTVSR
CCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RVKKGLDLLKHLMCVADD
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTTATNELKYEIWQLLREYQQSRSETVRNQLVKLNLGLVRKEAHYWMNQCRENYDDLVQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGCLGLIRAIERFEIAKGHAFSSFAIPYIRGEIQHYLRDKGVTVRIPRRYLAIQQRAIGV
HHHHHHHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHH
SRSLREQYNRQPTDSELATALEISPDEWQEIKLAWVNRAPLSLDVPVQDGEEGATSLGEL
HHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHEECCCCCEEECCCCCCCHHHHHHHHH
VPDHHYRSFQLAQEDQIRLQQALFQLEQRTREVLECVFLQDLTQKQVAEHLGISVVTVSR
CCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RVKKGLDLLKHLMCVADD
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1391049 [H]