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The map label for this gene is def2

Identifier: 17229499

GI number: 17229499

Start: 2400694

End: 2401233

Strand: Reverse

Name: def2

Synonym: all2007

Alternate gene names: 17229499

Gene position: 2401233-2400694 (Counterclockwise)

Preceding gene: 17229500

Following gene: 17229498

Centisome position: 37.44

GC content: 40.37

Gene sequence:

>540_bases
ATGAATCCCGAATTGCTCCCCATCATTCAATTAGGCAATCCCACGTTACGCCAAAAAGCTGCTTGGGTGGAAAATATTCA
TGATGCAACTATACAGCAATTAATTGATGACTTAATTGCCACAGTTGCCAAGGCTAACGGCGTGGGAATCGCATCTCCTC
AAGTAGCACAGTCTTATCGTTTATTTATCGTCGCTTCTCGTCCTAACCCCAGATATCCTCATGCTCCAGAAATGGAACCA
ACCGCCATGATCAACCCTAAGATAGTGGGACATTCGACAGAGATAGTCGAAGGTTGGGAAGGTTGTCTTAGTGTTCCGGG
AATTAGAGGATTGGTTCCTCGGCATCAAGCAATTGAGGTCGAATACACGGATCGTTATGGAAATTTACAAAAACAAACCT
TAACTGATTTTGTTGCTCGTATATTTCAACACGAATTTGATCATTTAGATGGTGTACTGTTTATAGACCGCGTAGAAAGT
AATTTAAATACAATTACGGAGGAAGAGTACCAAGAATTAGTAACTAAAAATACAAAATAA

Upstream 100 bases:

>100_bases
AATCTGAAATTGTTACCATGTACTTAGTATCAGAGCGTAGAGTATTACAATTCCCGATTACCCAATCCCCAATCCCCAGT
CCCCAGTCCCCATTCCCCAA

Downstream 100 bases:

>100_bases
AAATTCTTTTTAAGAATTAAACCCAACAAGTTTTTTTAATGTATACTTAACCTATTGTTAACCTAGAAAGCCGGGTGTAG
CTATCGAGTAAATGGCAAAA

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MNPELLPIIQLGNPTLRQKAAWVENIHDATIQQLIDDLIATVAKANGVGIASPQVAQSYRLFIVASRPNPRYPHAPEMEP
TAMINPKIVGHSTEIVEGWEGCLSVPGIRGLVPRHQAIEVEYTDRYGNLQKQTLTDFVARIFQHEFDHLDGVLFIDRVES
NLNTITEEEYQELVTKNTK

Sequences:

>Translated_179_residues
MNPELLPIIQLGNPTLRQKAAWVENIHDATIQQLIDDLIATVAKANGVGIASPQVAQSYRLFIVASRPNPRYPHAPEMEP
TAMINPKIVGHSTEIVEGWEGCLSVPGIRGLVPRHQAIEVEYTDRYGNLQKQTLTDFVARIFQHEFDHLDGVLFIDRVES
NLNTITEEEYQELVTKNTK
>Mature_179_residues
MNPELLPIIQLGNPTLRQKAAWVENIHDATIQQLIDDLIATVAKANGVGIASPQVAQSYRLFIVASRPNPRYPHAPEMEP
TAMINPKIVGHSTEIVEGWEGCLSVPGIRGLVPRHQAIEVEYTDRYGNLQKQTLTDFVARIFQHEFDHLDGVLFIDRVES
NLNTITEEEYQELVTKNTK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=168, Percent_Identity=31.547619047619, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1789682, Length=153, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24645728, Length=172, Percent_Identity=32.5581395348837, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24645726, Length=168, Percent_Identity=32.1428571428571, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF2_NOSS1 (Q8YVH1)

Other databases:

- EMBL:   BA000019
- PIR:   AI2056
- RefSeq:   NP_486047.1
- ProteinModelPortal:   Q8YVH1
- SMR:   Q8YVH1
- STRING:   Q8YVH1
- GeneID:   1105602
- GenomeReviews:   BA000019_GR
- KEGG:   ana:all2007
- NMPDR:   fig|103690.1.peg.2314
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   DFPARIF
- ProtClustDB:   CLSK895652
- BioCyc:   NSP103690:ALL2007-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20136; Mature: 20136

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: ACT_SITE 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPELLPIIQLGNPTLRQKAAWVENIHDATIQQLIDDLIATVAKANGVGIASPQVAQSYR
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEE
LFIVASRPNPRYPHAPEMEPTAMINPKIVGHSTEIVEGWEGCLSVPGIRGLVPRHQAIEV
EEEEECCCCCCCCCCCCCCCCEECCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEE
EYTDRYGNLQKQTLTDFVARIFQHEFDHLDGVLFIDRVESNLNTITEEEYQELVTKNTK
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNPELLPIIQLGNPTLRQKAAWVENIHDATIQQLIDDLIATVAKANGVGIASPQVAQSYR
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEE
LFIVASRPNPRYPHAPEMEPTAMINPKIVGHSTEIVEGWEGCLSVPGIRGLVPRHQAIEV
EEEEECCCCCCCCCCCCCCCCEECCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEE
EYTDRYGNLQKQTLTDFVARIFQHEFDHLDGVLFIDRVESNLNTITEEEYQELVTKNTK
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840