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The map label for this gene is lipA1

Identifier: 17229186

GI number: 17229186

Start: 2023824

End: 2024699

Strand: Reverse

Name: lipA1

Synonym: all1694

Alternate gene names: 17229186

Gene position: 2024699-2023824 (Counterclockwise)

Preceding gene: 17229187

Following gene: 17229185

Centisome position: 31.57

GC content: 47.49

Gene sequence:

>876_bases
GTGACTGTAAAACCAGACTGGTTGCGGGTAAAAGCGCCTCAATGGGAGCGCGTTGGTAACGTTAAAGAAATTTTGCGGGA
TTTAGCGCTAAATACAGTTTGTGAGGAAGCGTCATGTCCAAACATTGGTGAATGCTTCCAAGCTGGTACAGCCACATTTT
TGATTATGGGGCCAGCCTGCACCCGTGCTTGTCCTTATTGTGATATTGATTTTGAGAAAAAACCCAAAGCGCTCGACCCC
ACAGAACCCACACGATTAGCCGAAGCTGTGCGGCGGATGAGACTGAATCATGTGGTCATCACTTCTGTGAACCGAGATGA
TTTACCAGATGGTGGTGCATCACAGTTTGTCAAGTGTATTGCAGAGGTGCGCGCGGTTTCGCCTCAAACCACAATTGAGG
TGCTAATCCCTGATTTGTGCGGTAATTGGGAGGCGTTAAGGATCATTCTCCAAGCATCACCAGAGGTATTAAACCATAAC
ACAGAAACGATTCCTCGCCTTTATCGACGAGTCCGACCCCAAGGTAACTACGATCGCACCCTAGAATTATTGCAGCGATC
GCGTCAAATTGCTCCCTGGCTTTACACCAAATCCGGTATCATGGTCGGCTTAGGCGAAACTAATGCCGAAGTTCGCCAAG
TGATGCAAGACCTACGTGCTGTGGATTGCGATATCCTAACAATCGGGCAGTATCTCCAACCCAGTCAAAAGCACTTGCAA
GTCAACGATTTCGTTACTCCAGAGCAATTTGCTGCTTGGCAAGTTTATGGTGAAGAACTCGGATTTTTACAAGTTGTCTC
CTCACCTTTGACGAGAAGTTCTTACCACGCCGAACAAGTACGACAATTAATGGAGCGTTACCCACGGAACAAGTAG

Upstream 100 bases:

>100_bases
CGAAAAGATATCTACATCTATATATTATATATAGATGAATCGGAACGGGATTTGCTTGCTAGGATTAGATTTGTATAACA
TTCAGTGGTAAAAGCGGATT

Downstream 100 bases:

>100_bases
AAGCGTAATCAATCGTGTCTCTACAATTTTGAATTGAGAATTTTTAAATCGGTGCAAGAAACTACTATATCGATTTTGGG
TGCAGGTGCTTGGGGTGCAT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTVKPDWLRVKAPQWERVGNVKEILRDLALNTVCEEASCPNIGECFQAGTATFLIMGPACTRACPYCDIDFEKKPKALDP
TEPTRLAEAVRRMRLNHVVITSVNRDDLPDGGASQFVKCIAEVRAVSPQTTIEVLIPDLCGNWEALRIILQASPEVLNHN
TETIPRLYRRVRPQGNYDRTLELLQRSRQIAPWLYTKSGIMVGLGETNAEVRQVMQDLRAVDCDILTIGQYLQPSQKHLQ
VNDFVTPEQFAAWQVYGEELGFLQVVSSPLTRSSYHAEQVRQLMERYPRNK

Sequences:

>Translated_291_residues
MTVKPDWLRVKAPQWERVGNVKEILRDLALNTVCEEASCPNIGECFQAGTATFLIMGPACTRACPYCDIDFEKKPKALDP
TEPTRLAEAVRRMRLNHVVITSVNRDDLPDGGASQFVKCIAEVRAVSPQTTIEVLIPDLCGNWEALRIILQASPEVLNHN
TETIPRLYRRVRPQGNYDRTLELLQRSRQIAPWLYTKSGIMVGLGETNAEVRQVMQDLRAVDCDILTIGQYLQPSQKHLQ
VNDFVTPEQFAAWQVYGEELGFLQVVSSPLTRSSYHAEQVRQLMERYPRNK
>Mature_290_residues
TVKPDWLRVKAPQWERVGNVKEILRDLALNTVCEEASCPNIGECFQAGTATFLIMGPACTRACPYCDIDFEKKPKALDPT
EPTRLAEAVRRMRLNHVVITSVNRDDLPDGGASQFVKCIAEVRAVSPQTTIEVLIPDLCGNWEALRIILQASPEVLNHNT
ETIPRLYRRVRPQGNYDRTLELLQRSRQIAPWLYTKSGIMVGLGETNAEVRQVMQDLRAVDCDILTIGQYLQPSQKHLQV
NDFVTPEQFAAWQVYGEELGFLQVVSSPLTRSSYHAEQVRQLMERYPRNK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=281, Percent_Identity=41.9928825622776, Blast_Score=258, Evalue=4e-69,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=41.3934426229508, Blast_Score=219, Evalue=2e-57,
Organism=Escherichia coli, GI1786846, Length=284, Percent_Identity=48.2394366197183, Blast_Score=287, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI32564533, Length=252, Percent_Identity=43.2539682539683, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324770, Length=282, Percent_Identity=40.0709219858156, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI221513272, Length=279, Percent_Identity=43.7275985663082, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA1_NOSS1 (Q8YWC1)

Other databases:

- EMBL:   BA000019
- PIR:   AH2017
- RefSeq:   NP_485734.1
- ProteinModelPortal:   Q8YWC1
- STRING:   Q8YWC1
- GeneID:   1105286
- GenomeReviews:   BA000019_GR
- KEGG:   ana:all1694
- NMPDR:   fig|103690.1.peg.2001
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q8YWC1
- ProtClustDB:   PRK05481
- BioCyc:   NSP103690:ALL1694-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 33051; Mature: 32920

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKPDWLRVKAPQWERVGNVKEILRDLALNTVCEEASCPNIGECFQAGTATFLIMGPAC
CCCCCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCHH
TRACPYCDIDFEKKPKALDPTEPTRLAEAVRRMRLNHVVITSVNRDDLPDGGASQFVKCI
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHH
AEVRAVSPQTTIEVLIPDLCGNWEALRIILQASPEVLNHNTETIPRLYRRVRPQGNYDRT
HHHHHCCCCCHHEEEEHHHHCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCHHHH
LELLQRSRQIAPWLYTKSGIMVGLGETNAEVRQVMQDLRAVDCDILTIGQYLQPSQKHLQ
HHHHHHHHCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCEEHHHHCCCHHHCEE
VNDFVTPEQFAAWQVYGEELGFLQVVSSPLTRSSYHAEQVRQLMERYPRNK
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVKPDWLRVKAPQWERVGNVKEILRDLALNTVCEEASCPNIGECFQAGTATFLIMGPAC
CCCCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCHH
TRACPYCDIDFEKKPKALDPTEPTRLAEAVRRMRLNHVVITSVNRDDLPDGGASQFVKCI
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHH
AEVRAVSPQTTIEVLIPDLCGNWEALRIILQASPEVLNHNTETIPRLYRRVRPQGNYDRT
HHHHHCCCCCHHEEEEHHHHCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCHHHH
LELLQRSRQIAPWLYTKSGIMVGLGETNAEVRQVMQDLRAVDCDILTIGQYLQPSQKHLQ
HHHHHHHHCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCEEHHHHCCCHHHCEE
VNDFVTPEQFAAWQVYGEELGFLQVVSSPLTRSSYHAEQVRQLMERYPRNK
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840