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The map label for this gene is thiE [H]

Identifier: 16767417

GI number: 16767417

Start: 4379856

End: 4380491

Strand: Reverse

Name: thiE [H]

Synonym: STM4163

Alternate gene names: 16767417

Gene position: 4380491-4379856 (Counterclockwise)

Preceding gene: 16767418

Following gene: 16767416

Centisome position: 90.18

GC content: 59.59

Gene sequence:

>636_bases
ATGTATCAGCCTGATTTCCCCACCGTGCCGTTCCGCCTCGGGCTTTACCCGGTGGTGGACAGCGTTGAATGGATTGAGCG
TTTACTGGAGTCGGGCGTGCGCACGATCCAGTTGCGTATCAAAGATAAACGCGATGAAGAGGTGGAAGCCGACGTTATCG
CCGCCATCGCGCTGGGGCGTCGTTATAACGCCCGGCTGTTTATCAACGATTACTGGCGTCTGGCAATTAAGCACCGCGCT
TACGGCGTGCATCTGGGCCAGGAAGACCTTGAAACCACCGACCTGAAAGCGATTCAAGCGGCGGGCTTACGCCTGGGCGT
ATCGACCCACGATGATATGGAGATAGACGTCGCGCTCGCCGCTAAGCCTTCTTATATCGCGCTGGGTCATGTCTTCCCCA
CGCAAACCAAGCAGATGCCTTCCGCCCCGCAGGGGCTGGCGCAGCTGGCCAGTCATATTGAACGACTGGCGGATTACCCG
ACCGTGGCAATTGGCGGCATCAGCCTTGAACGCGCTCCGGCGGTACTGGCGACCGGCGTCGGCAGCATTGCCGTGGTCAG
CGCTATTACTCAGGCCGCAGACTGGCGTGAAGCCACTGCAGAGCTGCTGGCAATCGCAGGAGTCGGCGATGAATGA

Upstream 100 bases:

>100_bases
ACTACGCCGCCGCACAAACTATTGAAATCGGCATGGCGGATATGTCAGAGGACTTCCGCGCCAAAGGCGGCGAAATTTAT
CTCAAGCGGGAGGAAGCCTG

Downstream 100 bases:

>100_bases
CCATGATTTTATGCGTTATAGCCGCCAGATCCTGCTCGGCGATATCGCGATTGAAGGCCAGCAAAAGTTGCTCAATAGCC
ATGTATTGATTGTCGGCTTA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MYQPDFPTVPFRLGLYPVVDSVEWIERLLESGVRTIQLRIKDKRDEEVEADVIAAIALGRRYNARLFINDYWRLAIKHRA
YGVHLGQEDLETTDLKAIQAAGLRLGVSTHDDMEIDVALAAKPSYIALGHVFPTQTKQMPSAPQGLAQLASHIERLADYP
TVAIGGISLERAPAVLATGVGSIAVVSAITQAADWREATAELLAIAGVGDE

Sequences:

>Translated_211_residues
MYQPDFPTVPFRLGLYPVVDSVEWIERLLESGVRTIQLRIKDKRDEEVEADVIAAIALGRRYNARLFINDYWRLAIKHRA
YGVHLGQEDLETTDLKAIQAAGLRLGVSTHDDMEIDVALAAKPSYIALGHVFPTQTKQMPSAPQGLAQLASHIERLADYP
TVAIGGISLERAPAVLATGVGSIAVVSAITQAADWREATAELLAIAGVGDE
>Mature_211_residues
MYQPDFPTVPFRLGLYPVVDSVEWIERLLESGVRTIQLRIKDKRDEEVEADVIAAIALGRRYNARLFINDYWRLAIKHRA
YGVHLGQEDLETTDLKAIQAAGLRLGVSTHDDMEIDVALAAKPSYIALGHVFPTQTKQMPSAPQGLAQLASHIERLADYP
TVAIGGISLERAPAVLATGVGSIAVVSAITQAADWREATAELLAIAGVGDE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=211, Percent_Identity=89.5734597156398, Blast_Score=387, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6325042, Length=214, Percent_Identity=29.9065420560748, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22984; Mature: 22984

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQPDFPTVPFRLGLYPVVDSVEWIERLLESGVRTIQLRIKDKRDEEVEADVIAAIALGR
CCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHCC
RYNARLFINDYWRLAIKHRAYGVHLGQEDLETTDLKAIQAAGLRLGVSTHDDMEIDVALA
CCCCEEEEEHHEEEEEHHHEEEEECCCHHCCHHHHHHHHHCCEEEECCCCCCCEEEEEEE
AKPSYIALGHVFPTQTKQMPSAPQGLAQLASHIERLADYPTVAIGGISLERAPAVLATGV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHCC
GSIAVVSAITQAADWREATAELLAIAGVGDE
CHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
>Mature Secondary Structure
MYQPDFPTVPFRLGLYPVVDSVEWIERLLESGVRTIQLRIKDKRDEEVEADVIAAIALGR
CCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHCC
RYNARLFINDYWRLAIKHRAYGVHLGQEDLETTDLKAIQAAGLRLGVSTHDDMEIDVALA
CCCCEEEEEHHEEEEEHHHEEEEECCCHHCCHHHHHHHHHCCEEEECCCCCCCEEEEEEE
AKPSYIALGHVFPTQTKQMPSAPQGLAQLASHIERLADYPTVAIGGISLERAPAVLATGV
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHCC
GSIAVVSAITQAADWREATAELLAIAGVGDE
CHHHHHHHHHHHHHHHHHHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA