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The map label for this gene is thiF [H]

Identifier: 16767416

GI number: 16767416

Start: 4379105

End: 4379863

Strand: Reverse

Name: thiF [H]

Synonym: STM4162

Alternate gene names: 16767416

Gene position: 4379863-4379105 (Counterclockwise)

Preceding gene: 16767417

Following gene: 16767415

Centisome position: 90.17

GC content: 57.84

Gene sequence:

>759_bases
ATGAATGACCATGATTTTATGCGTTATAGCCGCCAGATCCTGCTCGGCGATATCGCGATTGAAGGCCAGCAAAAGTTGCT
CAATAGCCATGTATTGATTGTCGGCTTAGGCGGATTGGGTTCGCCTGCCGCGCTGTATCTGGCGGGAGCAGGCATCGGCA
AACTAACGCTGGTAGACGATGACGATGTTCACTTGAGCAATCTCCAGCGGCAAATTCTGTTCACCACCGACGATATCGCC
CACCCGAAGGCGCAGGCGGCAAAACTACGGCTGGCGCAGCTCAACCCCGGTAGCAAGCTGATCGTATTGCAACAACGTCT
GACTGGCGATGTGCTTAAAAACGCGGTAGCACACGCCGACGTAGTGCTCGACTGTACCGATAACATGGCGACGCGCCAGG
AGATTAACGCCGCCTGTGTGGCGCTCAACACTCCGTTAATTTCCGCCAGCGCCGTCGGCTTTGGCGGCCAGCTAATGGTC
CTCACACCACCGTGGGAACAAGGCTGTTACCGCTGCCTGTGGCCGGACGATGTCGAGCCTGAACGCAACTGCCGTACCGC
CGGTATCGTCGGACCGGTTGTTGGCGTGATGGGCACCTTGCAGGCGCTGGAGGCAATCAAATTACTCAGCGGTATTGAGA
CGCCCAGCGGAGAGCTACGCCTGTTTGACGGCAAAACCAGCCAGTGGCGCAGCCTGGCGCTGCGTCATGCCAGCGGCTGT
CCGGTCTGCGGAGGGCAACATGCAGATTCAGTTCAATGA

Upstream 100 bases:

>100_bases
GCGACCGGCGTCGGCAGCATTGCCGTGGTCAGCGCTATTACTCAGGCCGCAGACTGGCGTGAAGCCACTGCAGAGCTGCT
GGCAATCGCAGGAGTCGGCG

Downstream 100 bases:

>100_bases
TGAACCGATGCAGTGCGCCGCAGGGCAAACCGTCAGTGAGCTGCTCACGCAACTCAATCAGCTTAAACCGGGCGCGGCGC
TGGCGCTCAATCAGCAGATC

Product: thiamine biosynthesis protein ThiF

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNDHDFMRYSRQILLGDIAIEGQQKLLNSHVLIVGLGGLGSPAALYLAGAGIGKLTLVDDDDVHLSNLQRQILFTTDDIA
HPKAQAAKLRLAQLNPGSKLIVLQQRLTGDVLKNAVAHADVVLDCTDNMATRQEINAACVALNTPLISASAVGFGGQLMV
LTPPWEQGCYRCLWPDDVEPERNCRTAGIVGPVVGVMGTLQALEAIKLLSGIETPSGELRLFDGKTSQWRSLALRHASGC
PVCGGQHADSVQ

Sequences:

>Translated_252_residues
MNDHDFMRYSRQILLGDIAIEGQQKLLNSHVLIVGLGGLGSPAALYLAGAGIGKLTLVDDDDVHLSNLQRQILFTTDDIA
HPKAQAAKLRLAQLNPGSKLIVLQQRLTGDVLKNAVAHADVVLDCTDNMATRQEINAACVALNTPLISASAVGFGGQLMV
LTPPWEQGCYRCLWPDDVEPERNCRTAGIVGPVVGVMGTLQALEAIKLLSGIETPSGELRLFDGKTSQWRSLALRHASGC
PVCGGQHADSVQ
>Mature_252_residues
MNDHDFMRYSRQILLGDIAIEGQQKLLNSHVLIVGLGGLGSPAALYLAGAGIGKLTLVDDDDVHLSNLQRQILFTTDDIA
HPKAQAAKLRLAQLNPGSKLIVLQQRLTGDVLKNAVAHADVVLDCTDNMATRQEINAACVALNTPLISASAVGFGGQLMV
LTPPWEQGCYRCLWPDDVEPERNCRTAGIVGPVVGVMGTLQALEAIKLLSGIETPSGELRLFDGKTSQWRSLALRHASGC
PVCGGQHADSVQ

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=228, Percent_Identity=43.421052631579, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI4885649, Length=144, Percent_Identity=30.5555555555556, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI38045948, Length=163, Percent_Identity=33.1288343558282, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI87082356, Length=251, Percent_Identity=84.0637450199203, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1787048, Length=245, Percent_Identity=44.0816326530612, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1789177, Length=152, Percent_Identity=33.5526315789474, Blast_Score=77, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17540406, Length=248, Percent_Identity=41.9354838709677, Blast_Score=190, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI193203301, Length=154, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321903, Length=249, Percent_Identity=36.144578313253, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320598, Length=165, Percent_Identity=31.5151515151515, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322639, Length=167, Percent_Identity=27.5449101796407, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582879, Length=251, Percent_Identity=44.2231075697211, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24660640, Length=155, Percent_Identity=32.258064516129, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28573937, Length=164, Percent_Identity=27.4390243902439, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 26891; Mature: 26891

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDHDFMRYSRQILLGDIAIEGQQKLLNSHVLIVGLGGLGSPAALYLAGAGIGKLTLVDD
CCCHHHHHHHHHEEEEEEEECCHHHHHCCCEEEEEECCCCCCCEEEEECCCCCEEEEECC
DDVHLSNLQRQILFTTDDIAHPKAQAAKLRLAQLNPGSKLIVLQQRLTGDVLKNAVAHAD
CCCHHHHHCEEEEEEECCCCCCCHHHHHEEEEEECCCCEEEEEHHHHHHHHHHHHHHHCE
VVLDCTDNMATRQEINAACVALNTPLISASAVGFGGQLMVLTPPWEQGCYRCLWPDDVEP
EEEECCCCHHHHHHHCEEEEEECCCCEEEEECCCCCEEEEECCCHHHCCEEECCCCCCCC
ERNCRTAGIVGPVVGVMGTLQALEAIKLLSGIETPSGELRLFDGKTSQWRSLALRHASGC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCC
PVCGGQHADSVQ
CCCCCCCCCCCC
>Mature Secondary Structure
MNDHDFMRYSRQILLGDIAIEGQQKLLNSHVLIVGLGGLGSPAALYLAGAGIGKLTLVDD
CCCHHHHHHHHHEEEEEEEECCHHHHHCCCEEEEEECCCCCCCEEEEECCCCCEEEEECC
DDVHLSNLQRQILFTTDDIAHPKAQAAKLRLAQLNPGSKLIVLQQRLTGDVLKNAVAHAD
CCCHHHHHCEEEEEEECCCCCCCHHHHHEEEEEECCCCEEEEEHHHHHHHHHHHHHHHCE
VVLDCTDNMATRQEINAACVALNTPLISASAVGFGGQLMVLTPPWEQGCYRCLWPDDVEP
EEEECCCCHHHHHHHCEEEEEECCCCEEEEECCCCCEEEEECCCHHHCCEEECCCCCCCC
ERNCRTAGIVGPVVGVMGTLQALEAIKLLSGIETPSGELRLFDGKTSQWRSLALRHASGC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCC
PVCGGQHADSVQ
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]