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The map label for this gene is hisH [H]

Identifier: 15921758

GI number: 15921758

Start: 1468476

End: 1469069

Strand: Direct

Name: hisH [H]

Synonym: ST1466

Alternate gene names: 15921758

Gene position: 1468476-1469069 (Clockwise)

Preceding gene: 15921757

Following gene: 15921759

Centisome position: 54.49

GC content: 34.85

Gene sequence:

>594_bases
GTGATGAAAGCTACTCTAATAAACTATGGTGTAGGAAACCTGTTCAGCATTAAGGCTGGTCTAGAAAGGGTTGGGTTTAA
TGTTAAGATTTCTTTCTTACCAGAGGGAGATGAAGATGTCATAGTATTGCCTGGTGTAGGTGCGTTTTCTGCAGTCTCTT
CCTATCTAAATTCTATGAAGGATAAATTTAATGAACTGAGAGAGAGAGGAGTAAAGTTTTTGGGTGTATGTTTAGGTATG
CAAGTAATGTTTGATGAAGGAACTGAGGGGGGTTTGAGTAAAGGTTTAGGATGGTTTAAAGGTAAAGTTGATAAAATTCA
TTCTAACGTAAAACTTCCTCATATTGGTTGGGACAAGTTATTTGTTAATAAAGATTCTTGTAATTTGACTGAAGGCTTAG
ATGGTAAATATGTGTATTATGTCCATAGTTATGTAGCGTATACAAATGATTACGTTGCTTATAGTGAGTATGGAATTAAA
TACCCAGCTATAGTATGTAATGATTTTGCTGTAGGAACTCAGTTTCATCCTGAAAAGAGCAGTGTAACTGGTAAGATATT
CCTTAGAAACTTTTATTCGTGGGTTAAAAGATGA

Upstream 100 bases:

>100_bases
TTAGTGAAGTAGCAGACTTAATATATCATCTTTTCGTATTAATGGCTGTAGAAGGAGTTAAGCCAGAAGATGTTTATGAG
GAGCTAAAAAGGAGGATGAA

Downstream 100 bases:

>100_bases
TGAAACTAAGTGAGAATGAAGCCTCTAAGTTAATAGAAAAATTATGGTTTAGGCATACAGATTCTACTATAATAGCTGTT
CTTCAAGATTATAAAACTAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MMKATLINYGVGNLFSIKAGLERVGFNVKISFLPEGDEDVIVLPGVGAFSAVSSYLNSMKDKFNELRERGVKFLGVCLGM
QVMFDEGTEGGLSKGLGWFKGKVDKIHSNVKLPHIGWDKLFVNKDSCNLTEGLDGKYVYYVHSYVAYTNDYVAYSEYGIK
YPAIVCNDFAVGTQFHPEKSSVTGKIFLRNFYSWVKR

Sequences:

>Translated_197_residues
MMKATLINYGVGNLFSIKAGLERVGFNVKISFLPEGDEDVIVLPGVGAFSAVSSYLNSMKDKFNELRERGVKFLGVCLGM
QVMFDEGTEGGLSKGLGWFKGKVDKIHSNVKLPHIGWDKLFVNKDSCNLTEGLDGKYVYYVHSYVAYTNDYVAYSEYGIK
YPAIVCNDFAVGTQFHPEKSSVTGKIFLRNFYSWVKR
>Mature_197_residues
MMKATLINYGVGNLFSIKAGLERVGFNVKISFLPEGDEDVIVLPGVGAFSAVSSYLNSMKDKFNELRERGVKFLGVCLGM
QVMFDEGTEGGLSKGLGWFKGKVDKIHSNVKLPHIGWDKLFVNKDSCNLTEGLDGKYVYYVHSYVAYTNDYVAYSEYGIK
YPAIVCNDFAVGTQFHPEKSSVTGKIFLRNFYSWVKR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=195, Percent_Identity=32.8205128205128, Blast_Score=100, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=206, Percent_Identity=30.5825242718447, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22050; Mature: 22050

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKATLINYGVGNLFSIKAGLERVGFNVKISFLPEGDEDVIVLPGVGAFSAVSSYLNSMK
CCCEEEEECCCCCEEEHHHHHHHCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHH
DKFNELRERGVKFLGVCLGMQVMFDEGTEGGLSKGLGWFKGKVDKIHSNVKLPHIGWDKL
HHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHCCHHHHCHHHHHHCCCCCCCCCCCEE
FVNKDSCNLTEGLDGKYVYYVHSYVAYTNDYVAYSEYGIKYPAIVCNDFAVGTQFHPEKS
EEECCCCCCCCCCCCCEEEEEEEEHHHCCCEEEECCCCCCCCEEEECCEECCCEECCCCC
SVTGKIFLRNFYSWVKR
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMKATLINYGVGNLFSIKAGLERVGFNVKISFLPEGDEDVIVLPGVGAFSAVSSYLNSMK
CCCEEEEECCCCCEEEHHHHHHHCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHH
DKFNELRERGVKFLGVCLGMQVMFDEGTEGGLSKGLGWFKGKVDKIHSNVKLPHIGWDKL
HHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHCCHHHHCHHHHHHCCCCCCCCCCCEE
FVNKDSCNLTEGLDGKYVYYVHSYVAYTNDYVAYSEYGIKYPAIVCNDFAVGTQFHPEKS
EEECCCCCCCCCCCCCEEEEEEEEHHHCCCEEEECCCCCCCCEEEECCEECCCEECCCCC
SVTGKIFLRNFYSWVKR
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]