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The map label for this gene is 15920956

Identifier: 15920956

GI number: 15920956

Start: 709740

End: 711224

Strand: Direct

Name: 15920956

Synonym: ST0726

Alternate gene names: NA

Gene position: 709740-711224 (Clockwise)

Preceding gene: 15920954

Following gene: 15920957

Centisome position: 26.34

GC content: 36.16

Gene sequence:

>1485_bases
GTGGTCTATTTGATTAGGAAAGGAGAAGACTATATAAAAAGTATAAAGGCCCATCATCCAGTAGTCTATTATGAAGGAGA
AGTAGTAGAAGATATAACTGAACATCAGGCGTTTAAAATACCGGTGAGAACAGTAGCTAAATACTACGACCTTCACTGGG
ATCCAGAATACTCCAAATACCTAAGAGTATATAACCCCGATGTAGGTGAAGAGACCAGCATTAGCTTTTTAAGACCAAGA
AATAAGCAAGATTTAGCTAAGTTAAGGGACGGTTTAATAAAAATATATGATTTCTATAGAGGATTTTTCGGAAGAAGCCC
AGATTATCTAAACGTTTGGACCACAGTATTTTATGCCCATGCAGAGGATTACTTTGGAAAGCATTTCGGCTCTAAGTTCA
TGGAGAACATGATAGAGATTTACAGAGAAGCTACTAAAAATGACTTATTCTATACCCATGCAATAGTGGCACCGATGTAT
GATAGATCGAGACCGCCATCACAATGGGAAGACCCCTATATACAAGTTGGGATTGTTGAAGAGAAACCTGATGGTGTTAT
AGTAAGAGGAGCAGCTATGCTAAGTACTGCTGGTCCCTATTCGGAAATGTTATGGTATTTACCGAATATTAGGAGGGATA
CTGATCCTAGATATGCAATATATTTCTCTATTCCTACAGAGACAAAAGGTGTTAAATTCTTAGCAAGAAGAGGATTTCCT
CCAAGGGAAGGAGGAGAATTTGAATACCCTATATCATCAAGATTTGAAGAGAGTGATGCTATACTAGTCTTTGATAATGT
TTTCGTACCCTACGATAGAATAATATTCTTCAAAAAACCAGAATTAATTGAAGATTTGATGTGGCACACTGTTAGTTTAA
GAGGATGGTTCAACTGGCATTTTGTTATACAACACTACTCTAGGCTTAAGTTCTTAGCAGGATTAGCAATAGCTGTAGCA
GAGGCTGCTGGGATAAATAATTTCATAAATGTCCAAGAAAAAATTGGTGAAATACTTATTTATGTTGCATTAAATGAGGC
TGCACTTTATGCTTCAGTTGAAAGGGCTCAAGAATTGCCAAATATAGCAAGACCTGATCCCTACATTTCTATTTCAGCAA
GTCATTTCAATATGAAGGCTGTTCCAAGGGCAAATGAAATTTTGAGATTAATATCAGCTGGTTCATCAATTCCAGTTCCT
GCTGGAATTAAAGATTTTGAAAACCCAGAAGAGAGGAGATTGTTAGATAAGTATATGGCAATGAAAGGGTTAAACGCTTT
AGAGAGAGTAAAGCTATTTAACTTATTATGGGATGTTATAGGATCTGAGTCTGGAATGAGGTATGAGCAATACGATAGGT
TTAGTAGAGGCGATCCGACAATAAGATGGGCACAGACATATACAGAGGTATTTAAAGATAGGAAAAATGAATTTGTAAAA
TTAGTTAAAGAAATAATGGATCAAATGCCAAATCCTAAAGCATAA

Upstream 100 bases:

>100_bases
AGTTTAGATATAGAAGTGATTTTTGGGGAAGACCAATTCCCCAATCTTGGTTAAGTGAATGGGTTCCCGTGGAGGATATT
TCCACGGGTAAATTAAAGGG

Downstream 100 bases:

>100_bases
GGTGTTATTCCGTTTGACAAAGTTTTTATCTTTTTTAGTTAATAACACTAGAAAGTTAGGAATTCTAAATGGAAATTATA
TATATGAAGTGAAGGATTTT

Product: phenol hydroxylase

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-HPA 3-monooxygenase large component [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MVYLIRKGEDYIKSIKAHHPVVYYEGEVVEDITEHQAFKIPVRTVAKYYDLHWDPEYSKYLRVYNPDVGEETSISFLRPR
NKQDLAKLRDGLIKIYDFYRGFFGRSPDYLNVWTTVFYAHAEDYFGKHFGSKFMENMIEIYREATKNDLFYTHAIVAPMY
DRSRPPSQWEDPYIQVGIVEEKPDGVIVRGAAMLSTAGPYSEMLWYLPNIRRDTDPRYAIYFSIPTETKGVKFLARRGFP
PREGGEFEYPISSRFEESDAILVFDNVFVPYDRIIFFKKPELIEDLMWHTVSLRGWFNWHFVIQHYSRLKFLAGLAIAVA
EAAGINNFINVQEKIGEILIYVALNEAALYASVERAQELPNIARPDPYISISASHFNMKAVPRANEILRLISAGSSIPVP
AGIKDFENPEERRLLDKYMAMKGLNALERVKLFNLLWDVIGSESGMRYEQYDRFSRGDPTIRWAQTYTEVFKDRKNEFVK
LVKEIMDQMPNPKA

Sequences:

>Translated_494_residues
MVYLIRKGEDYIKSIKAHHPVVYYEGEVVEDITEHQAFKIPVRTVAKYYDLHWDPEYSKYLRVYNPDVGEETSISFLRPR
NKQDLAKLRDGLIKIYDFYRGFFGRSPDYLNVWTTVFYAHAEDYFGKHFGSKFMENMIEIYREATKNDLFYTHAIVAPMY
DRSRPPSQWEDPYIQVGIVEEKPDGVIVRGAAMLSTAGPYSEMLWYLPNIRRDTDPRYAIYFSIPTETKGVKFLARRGFP
PREGGEFEYPISSRFEESDAILVFDNVFVPYDRIIFFKKPELIEDLMWHTVSLRGWFNWHFVIQHYSRLKFLAGLAIAVA
EAAGINNFINVQEKIGEILIYVALNEAALYASVERAQELPNIARPDPYISISASHFNMKAVPRANEILRLISAGSSIPVP
AGIKDFENPEERRLLDKYMAMKGLNALERVKLFNLLWDVIGSESGMRYEQYDRFSRGDPTIRWAQTYTEVFKDRKNEFVK
LVKEIMDQMPNPKA
>Mature_494_residues
MVYLIRKGEDYIKSIKAHHPVVYYEGEVVEDITEHQAFKIPVRTVAKYYDLHWDPEYSKYLRVYNPDVGEETSISFLRPR
NKQDLAKLRDGLIKIYDFYRGFFGRSPDYLNVWTTVFYAHAEDYFGKHFGSKFMENMIEIYREATKNDLFYTHAIVAPMY
DRSRPPSQWEDPYIQVGIVEEKPDGVIVRGAAMLSTAGPYSEMLWYLPNIRRDTDPRYAIYFSIPTETKGVKFLARRGFP
PREGGEFEYPISSRFEESDAILVFDNVFVPYDRIIFFKKPELIEDLMWHTVSLRGWFNWHFVIQHYSRLKFLAGLAIAVA
EAAGINNFINVQEKIGEILIYVALNEAALYASVERAQELPNIARPDPYISISASHFNMKAVPRANEILRLISAGSSIPVP
AGIKDFENPEERRLLDKYMAMKGLNALERVKLFNLLWDVIGSESGMRYEQYDRFSRGDPTIRWAQTYTEVFKDRKNEFVK
LVKEIMDQMPNPKA

Specific function: Utilizes FADH(2) supplied by hpaC, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 57645; Mature: 57645

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYLIRKGEDYIKSIKAHHPVVYYEGEVVEDITEHQAFKIPVRTVAKYYDLHWDPEYSKY
CEEEEECCHHHHHHHHHCCCEEEECCHHHHHHHHCCEEECHHHHHHHHHCCCCCCCHHHH
LRVYNPDVGEETSISFLRPRNKQDLAKLRDGLIKIYDFYRGFFGRSPDYLNVWTTVFYAH
EEEECCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AEDYFGKHFGSKFMENMIEIYREATKNDLFYTHAIVAPMYDRSRPPSQWEDPYIQVGIVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
EKPDGVIVRGAAMLSTAGPYSEMLWYLPNIRRDTDPRYAIYFSIPTETKGVKFLARRGFP
ECCCCEEEECHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCC
PREGGEFEYPISSRFEESDAILVFDNVFVPYDRIIFFKKPELIEDLMWHTVSLRGWFNWH
CCCCCCCCCCHHCCCCCCCEEEEEECCCCCCCCEEEEECHHHHHHHHHHHEEECCEEEHH
FVIQHYSRLKFLAGLAIAVAEAAGINNFINVQEKIGEILIYVALNEAALYASVERAQELP
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCC
NIARPDPYISISASHFNMKAVPRANEILRLISAGSSIPVPAGIKDFENPEERRLLDKYMA
CCCCCCCEEEEEECCCCEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MKGLNALERVKLFNLLWDVIGSESGMRYEQYDRFSRGDPTIRWAQTYTEVFKDRKNEFVK
HHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHH
LVKEIMDQMPNPKA
HHHHHHHHCCCCCC
>Mature Secondary Structure
MVYLIRKGEDYIKSIKAHHPVVYYEGEVVEDITEHQAFKIPVRTVAKYYDLHWDPEYSKY
CEEEEECCHHHHHHHHHCCCEEEECCHHHHHHHHCCEEECHHHHHHHHHCCCCCCCHHHH
LRVYNPDVGEETSISFLRPRNKQDLAKLRDGLIKIYDFYRGFFGRSPDYLNVWTTVFYAH
EEEECCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AEDYFGKHFGSKFMENMIEIYREATKNDLFYTHAIVAPMYDRSRPPSQWEDPYIQVGIVE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
EKPDGVIVRGAAMLSTAGPYSEMLWYLPNIRRDTDPRYAIYFSIPTETKGVKFLARRGFP
ECCCCEEEECHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCC
PREGGEFEYPISSRFEESDAILVFDNVFVPYDRIIFFKKPELIEDLMWHTVSLRGWFNWH
CCCCCCCCCCHHCCCCCCCEEEEEECCCCCCCCEEEEECHHHHHHHHHHHEEECCEEEHH
FVIQHYSRLKFLAGLAIAVAEAAGINNFINVQEKIGEILIYVALNEAALYASVERAQELP
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHCC
NIARPDPYISISASHFNMKAVPRANEILRLISAGSSIPVPAGIKDFENPEERRLLDKYMA
CCCCCCCEEEEEECCCCEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MKGLNALERVKLFNLLWDVIGSESGMRYEQYDRFSRGDPTIRWAQTYTEVFKDRKNEFVK
HHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHH
LVKEIMDQMPNPKA
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA