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The map label for this gene is nfi

Identifier: 15920798

GI number: 15920798

Start: 568220

End: 568810

Strand: Direct

Name: nfi

Synonym: ST0581

Alternate gene names: 15920798

Gene position: 568220-568810 (Clockwise)

Preceding gene: 15920797

Following gene: 15920799

Centisome position: 21.09

GC content: 30.29

Gene sequence:

>591_bases
ATGGAGGACTATATTATAGATTTTCTTATAAAATTTCAAAAGTTAATAGCAAAAAATGTGAAAATTCAGCATCTTGGAAT
AGAAAATGTTAAAAGAATTTGTGGAACAGATATTGCTTATAAGGGAAATATTGGATATGCTGTTGCTGTAAAAGAAGAAG
AAGGAAAAATTGAATATAATCTCGTAAAAGGGGATGTCTTTTTTCCTTATATACCAACATTTCTTTTCGTAAGAGAAGCT
CCCTTGATGATAAAAGCTGTGGAAAAATATGAGTGTGATTTAATTTTAGTAGATGGACATGGCTTAACACATCCTAGAAA
GAGTGGGATAGCTACAGTCATTGGTGTTTTATTAGATAAACCGACTATAGGTGTGGCTAAATCTAGGTTAACTGGAGATC
TAGTAGTAGAAAATAATATAACTTATGTTATTCTTCATGGTGAAAAGTTAGGTGTAAAAGTAGGAAAGTATTTTTTTAGC
ATAGGTAATAAGACGGATCTTTCTGACGTAATAGATTTAGCTAAGAAAGGATATCCTAAAGTATTGAAACTCGCGGATAA
ACTTTCAAAAGATTTGAAGAAAAAAGAATAA

Upstream 100 bases:

>100_bases
GTGGAGAAGCAATTGAAGAAGTAAGAAATTATAGGCCCGGGGCTGTACAAACTTATGAGCAACAATTGTTTCTCTTACAA
CTTGAAAAGATGAAGGAGAA

Downstream 100 bases:

>100_bases
TAAACATGTGTTCGTATTATAAATTATGAAAGTTGAACTACCTCCAATTCCAGAGGAAAAAGAAGTTTTACTTTCTCCCT
CTGACACAGCATTACTTATT

Product: hypothetical protein

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MEDYIIDFLIKFQKLIAKNVKIQHLGIENVKRICGTDIAYKGNIGYAVAVKEEEGKIEYNLVKGDVFFPYIPTFLFVREA
PLMIKAVEKYECDLILVDGHGLTHPRKSGIATVIGVLLDKPTIGVAKSRLTGDLVVENNITYVILHGEKLGVKVGKYFFS
IGNKTDLSDVIDLAKKGYPKVLKLADKLSKDLKKKE

Sequences:

>Translated_196_residues
MEDYIIDFLIKFQKLIAKNVKIQHLGIENVKRICGTDIAYKGNIGYAVAVKEEEGKIEYNLVKGDVFFPYIPTFLFVREA
PLMIKAVEKYECDLILVDGHGLTHPRKSGIATVIGVLLDKPTIGVAKSRLTGDLVVENNITYVILHGEKLGVKVGKYFFS
IGNKTDLSDVIDLAKKGYPKVLKLADKLSKDLKKKE
>Mature_196_residues
MEDYIIDFLIKFQKLIAKNVKIQHLGIENVKRICGTDIAYKGNIGYAVAVKEEEGKIEYNLVKGDVFFPYIPTFLFVREA
PLMIKAVEKYECDLILVDGHGLTHPRKSGIATVIGVLLDKPTIGVAKSRLTGDLVVENNITYVILHGEKLGVKVGKYFFS
IGNKTDLSDVIDLAKKGYPKVLKLADKLSKDLKKKE

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Escherichia coli, GI87082357, Length=152, Percent_Identity=33.5526315789474, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_SULTO (Q974T1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376467.1
- ProteinModelPortal:   Q974T1
- GeneID:   1458526
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0581
- NMPDR:   fig|273063.1.peg.635
- HOGENOM:   HBG716360
- OMA:   GEPREIC
- ProtClustDB:   CLSK803389
- BioCyc:   STOK273063:ST0581-MONOMER
- BRENDA:   3.1.21.7
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 21898; Mature: 21898

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDYIIDFLIKFQKLIAKNVKIQHLGIENVKRICGTDIAYKGNIGYAVAVKEEEGKIEYN
CCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCEEECCCCEEEEEECCCCCEEEE
LVKGDVFFPYIPTFLFVREAPLMIKAVEKYECDLILVDGHGLTHPRKSGIATVIGVLLDK
EEECCEEHHHHHHHHHHHCCCHHEEEHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHCC
PTIGVAKSRLTGDLVVENNITYVILHGEKLGVKVGKYFFSIGNKTDLSDVIDLAKKGYPK
CCCCHHHHCCCCCEEEECCEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHH
VLKLADKLSKDLKKKE
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEDYIIDFLIKFQKLIAKNVKIQHLGIENVKRICGTDIAYKGNIGYAVAVKEEEGKIEYN
CCHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCEEECCCCEEEEEECCCCCEEEE
LVKGDVFFPYIPTFLFVREAPLMIKAVEKYECDLILVDGHGLTHPRKSGIATVIGVLLDK
EEECCEEHHHHHHHHHHHCCCHHEEEHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHCC
PTIGVAKSRLTGDLVVENNITYVILHGEKLGVKVGKYFFSIGNKTDLSDVIDLAKKGYPK
CCCCHHHHCCCCCEEEECCEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHH
VLKLADKLSKDLKKKE
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479