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The map label for this gene is rutB [H]

Identifier: 15920799

GI number: 15920799

Start: 568836

End: 569438

Strand: Direct

Name: rutB [H]

Synonym: ST0582

Alternate gene names: 15920799

Gene position: 568836-569438 (Clockwise)

Preceding gene: 15920798

Following gene: 15920800

Centisome position: 21.11

GC content: 33.5

Gene sequence:

>603_bases
ATGAAAGTTGAACTACCTCCAATTCCAGAGGAAAAAGAAGTTTTACTTTCTCCCTCTGACACAGCATTACTTATTGTAGA
TATGCAAAATGATTTCGTAAGAAAGGAAGGTAAATTATATGTTCCTAATGCAGAGACTACAATACCAGCAATAAGGAATC
TAATAGATAAGGCAAGAAATGCAAATGCTCTGATAATATATACTCAAGATTGGCACATGAAAGATGACCCAGAATTTAAA
ATTTGGGGAGAACATGCACTAGCTGGTACTTGGGGAGCTGAAATAGTAGAAGAGCTTAAACCAGAAAAAGATGATTTTAT
AATAAAGAAATATCGTTATGATGCTTTCTTTGAGACACCCTTAGACTATATTTTAAGGGTTAAAGGAATTAAGAATATAA
TAGTAACTGGTACAGTAGCGAATATTTGTGTTTTACATACTGCTGGAAGTGCTGCGTTAAGATGGTATAATGTAGTTATG
GTAAAAGACGGGATTTCAGCGATAACGGAATTCGACTACTATGCAACACTAAGACAAGTAGATTTTCTATATAAAGGAAA
AATTACCGACTCATCTGGAGTAAAATTTATTACTAGAATGTAG

Upstream 100 bases:

>100_bases
TTAGCTAAGAAAGGATATCCTAAAGTATTGAAACTCGCGGATAAACTTTCAAAAGATTTGAAGAAAAAAGAATAATAAAC
ATGTGTTCGTATTATAAATT

Downstream 100 bases:

>100_bases
GTTTATTGGTTATGGATTATTACGCTATAGTGCATAATGATTTTGATGGTACTGCATCTGCTGCCGTATACGCTAGAGCT
GTAAATGATCTTCCTAAAAA

Product: isochorismatase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MKVELPPIPEEKEVLLSPSDTALLIVDMQNDFVRKEGKLYVPNAETTIPAIRNLIDKARNANALIIYTQDWHMKDDPEFK
IWGEHALAGTWGAEIVEELKPEKDDFIIKKYRYDAFFETPLDYILRVKGIKNIIVTGTVANICVLHTAGSAALRWYNVVM
VKDGISAITEFDYYATLRQVDFLYKGKITDSSGVKFITRM

Sequences:

>Translated_200_residues
MKVELPPIPEEKEVLLSPSDTALLIVDMQNDFVRKEGKLYVPNAETTIPAIRNLIDKARNANALIIYTQDWHMKDDPEFK
IWGEHALAGTWGAEIVEELKPEKDDFIIKKYRYDAFFETPLDYILRVKGIKNIIVTGTVANICVLHTAGSAALRWYNVVM
VKDGISAITEFDYYATLRQVDFLYKGKITDSSGVKFITRM
>Mature_200_residues
MKVELPPIPEEKEVLLSPSDTALLIVDMQNDFVRKEGKLYVPNAETTIPAIRNLIDKARNANALIIYTQDWHMKDDPEFK
IWGEHALAGTWGAEIVEELKPEKDDFIIKKYRYDAFFETPLDYILRVKGIKNIIVTGTVANICVLHTAGSAALRWYNVVM
VKDGISAITEFDYYATLRQVDFLYKGKITDSSGVKFITRM

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=208, Percent_Identity=29.8076923076923, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786811, Length=178, Percent_Identity=30.3370786516854, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87081970, Length=179, Percent_Identity=27.9329608938547, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 22819; Mature: 22819

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVELPPIPEEKEVLLSPSDTALLIVDMQNDFVRKEGKLYVPNAETTIPAIRNLIDKARN
CCCCCCCCCCCCEEEECCCCCEEEEEECCCCHHHCCCEEEECCCCCHHHHHHHHHHHHCC
ANALIIYTQDWHMKDDPEFKIWGEHALAGTWGAEIVEELKPEKDDFIIKKYRYDAFFETP
CCEEEEEECCCCCCCCCCEEEECCEEECCCHHHHHHHHHCCCCCCEEEEEEECCEEHHCH
LDYILRVKGIKNIIVTGTVANICVLHTAGSAALRWYNVVMVKDGISAITEFDYYATLRQV
HHHHHHHCCCCEEEEECCHHEEEEEECCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHH
DFLYKGKITDSSGVKFITRM
HHEEECCCCCCCCCEEEEEC
>Mature Secondary Structure
MKVELPPIPEEKEVLLSPSDTALLIVDMQNDFVRKEGKLYVPNAETTIPAIRNLIDKARN
CCCCCCCCCCCCEEEECCCCCEEEEEECCCCHHHCCCEEEECCCCCHHHHHHHHHHHHCC
ANALIIYTQDWHMKDDPEFKIWGEHALAGTWGAEIVEELKPEKDDFIIKKYRYDAFFETP
CCEEEEEECCCCCCCCCCEEEECCEEECCCHHHHHHHHHCCCCCCEEEEEEECCEEHHCH
LDYILRVKGIKNIIVTGTVANICVLHTAGSAALRWYNVVMVKDGISAITEFDYYATLRQV
HHHHHHHCCCCEEEEECCHHEEEEEECCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHH
DFLYKGKITDSSGVKFITRM
HHEEECCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA