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The map label for this gene is rfc

Identifier: 15920690

GI number: 15920690

Start: 476298

End: 477281

Strand: Reverse

Name: rfc

Synonym: ST0475

Alternate gene names: 15920690

Gene position: 477281-476298 (Counterclockwise)

Preceding gene: 15920691

Following gene: 15920689

Centisome position: 17.71

GC content: 34.15

Gene sequence:

>984_bases
ATGTCACTTGAGGAGGAAATTCTTTGGGCTGAAAAATATAGACCTAGAAGCCTTGATGACATAGTTAATCAAAAAGATAT
TGTAGAGAGATTAAAAAGATTCGTAAAAGATAAGAATATGCCCCATTTACTTTTCTCTGGTCCTCCCGGTACAGGGAAAA
CTACAGCCGCCTTAGCCTTAGTTCATGATTTATACGGTGACAATTATAGGCAATATTTTCTTGAACTTAATGCTAGTGAT
GAAAGAGGTATAGATGTTATAAGGAATAAAGTTAAGGAATTTGCTAGAACAGTAGCTGGGGGAAACGTACCATTTAAGGT
GGTGTTACTTGATGAAGCAGATAATATGACTGCTGATGCTCAACAAGCTTTAAGAAGAACCATGGAATTATATACAGAAA
CAACCCGTTTCATTTTAGCTTGTAATTATCTTAGTAAAATAATTGAACCTATACAATCAAGAACTGCACTTTTTAGGTTT
TATCCGTTAAAGAAAGAAGATGTAGTGGCTAGACTTGCATATATAGCTAAAAACGAGAAAGTAGAGTATGATCAGAAGGC
CCTTGAAACAATTTATGATATAACTCAAGGAGACATGAGAAAAGCGATAAATATACTTCAAGCTTCATCAGTATATGGTA
AAGTAACTGTAGAAGCTGTATACAAAGTTCTTGGACTTGCACAGCCAAAAGAAATAAGAGAAATGATTATGTTGGCACTA
CAAGGAAATTTCCTTAAGGCGAGAGAGAAGTTAAGAGAGTTATTAGTAAATTATGGATTGTCAGGTGAAGATATTATAAA
ACAAATACATAGAGAAGTTACAGGTAACGAAATAAATATTCCAGATGATTTAAAAGTATTACTAGTAGATTATATTGGTG
AGGTAGAATATAGAATAATGGAAGGGGCAGACGACGAGATCCAACTAAATGCTCTTCTAGCTAAACTGGCCGTTTACGGA
GAAAAGTACTTAAAAGGGAAGTAA

Upstream 100 bases:

>100_bases
AAAGGACATATAATTGATCAAGGAGTAATTATGGGTAAGTATAAAAGGAAGTAGCCCTTTTAACTATCTTTTTAGAATAA
TATATGTAAGGGTAAATTTC

Downstream 100 bases:

>100_bases
ATTGCCACTACAATGGTTTCTAAAATATAGGCCAAAGACTCTAAATGAAGTAGAGAACGAAGAAGATGCTAAAAAAGAAC
TAGTGGAATGGATAGAAAGT

Product: replication factor C small subunit

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC small subunit; Clamp loader small subunit

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSLEEEILWAEKYRPRSLDDIVNQKDIVERLKRFVKDKNMPHLLFSGPPGTGKTTAALALVHDLYGDNYRQYFLELNASD
ERGIDVIRNKVKEFARTVAGGNVPFKVVLLDEADNMTADAQQALRRTMELYTETTRFILACNYLSKIIEPIQSRTALFRF
YPLKKEDVVARLAYIAKNEKVEYDQKALETIYDITQGDMRKAINILQASSVYGKVTVEAVYKVLGLAQPKEIREMIMLAL
QGNFLKAREKLRELLVNYGLSGEDIIKQIHREVTGNEINIPDDLKVLLVDYIGEVEYRIMEGADDEIQLNALLAKLAVYG
EKYLKGK

Sequences:

>Translated_327_residues
MSLEEEILWAEKYRPRSLDDIVNQKDIVERLKRFVKDKNMPHLLFSGPPGTGKTTAALALVHDLYGDNYRQYFLELNASD
ERGIDVIRNKVKEFARTVAGGNVPFKVVLLDEADNMTADAQQALRRTMELYTETTRFILACNYLSKIIEPIQSRTALFRF
YPLKKEDVVARLAYIAKNEKVEYDQKALETIYDITQGDMRKAINILQASSVYGKVTVEAVYKVLGLAQPKEIREMIMLAL
QGNFLKAREKLRELLVNYGLSGEDIIKQIHREVTGNEINIPDDLKVLLVDYIGEVEYRIMEGADDEIQLNALLAKLAVYG
EKYLKGK
>Mature_326_residues
SLEEEILWAEKYRPRSLDDIVNQKDIVERLKRFVKDKNMPHLLFSGPPGTGKTTAALALVHDLYGDNYRQYFLELNASDE
RGIDVIRNKVKEFARTVAGGNVPFKVVLLDEADNMTADAQQALRRTMELYTETTRFILACNYLSKIIEPIQSRTALFRFY
PLKKEDVVARLAYIAKNEKVEYDQKALETIYDITQGDMRKAINILQASSVYGKVTVEAVYKVLGLAQPKEIREMIMLALQ
GNFLKAREKLRELLVNYGLSGEDIIKQIHREVTGNEINIPDDLKVLLVDYIGEVEYRIMEGADDEIQLNALLAKLAVYGE
KYLKGK

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcS subfamily

Homologues:

Organism=Homo sapiens, GI6677723, Length=312, Percent_Identity=42.6282051282051, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI194306571, Length=310, Percent_Identity=42.5806451612903, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI194306567, Length=310, Percent_Identity=42.5806451612903, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI31563534, Length=316, Percent_Identity=43.9873417721519, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI4506491, Length=318, Percent_Identity=41.1949685534591, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI31881687, Length=318, Percent_Identity=41.1949685534591, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI28882049, Length=313, Percent_Identity=38.6581469648562, Blast_Score=205, Evalue=5e-53,
Organism=Homo sapiens, GI194306569, Length=246, Percent_Identity=42.6829268292683, Blast_Score=198, Evalue=4e-51,
Organism=Homo sapiens, GI4506489, Length=353, Percent_Identity=29.4617563739377, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI108773789, Length=235, Percent_Identity=34.0425531914894, Blast_Score=127, Evalue=9e-30,
Organism=Escherichia coli, GI1786676, Length=238, Percent_Identity=27.7310924369748, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17541988, Length=313, Percent_Identity=45.3674121405751, Blast_Score=257, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI71986063, Length=312, Percent_Identity=41.025641025641, Blast_Score=251, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI17554730, Length=208, Percent_Identity=44.7115384615385, Blast_Score=192, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17541990, Length=287, Percent_Identity=33.1010452961672, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324478, Length=319, Percent_Identity=44.5141065830721, Blast_Score=260, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322528, Length=327, Percent_Identity=39.4495412844037, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6324039, Length=319, Percent_Identity=36.0501567398119, Blast_Score=229, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6319562, Length=253, Percent_Identity=29.6442687747036, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6324791, Length=235, Percent_Identity=28.5106382978723, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17647857, Length=319, Percent_Identity=47.0219435736677, Blast_Score=268, Evalue=3e-72,
Organism=Drosophila melanogaster, GI19921076, Length=314, Percent_Identity=38.8535031847134, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI18859927, Length=314, Percent_Identity=36.9426751592357, Blast_Score=221, Evalue=7e-58,
Organism=Drosophila melanogaster, GI19921136, Length=350, Percent_Identity=29.4285714285714, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCS_SULTO (Q975D3)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376359.1
- ProteinModelPortal:   Q975D3
- SMR:   Q975D3
- GeneID:   1458417
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0475
- NMPDR:   fig|273063.1.peg.527
- HOGENOM:   HBG541599
- OMA:   CDFRIVE
- ProtClustDB:   PRK00440
- BioCyc:   STOK273063:ST0475-MONOMER
- HAMAP:   MF_01509
- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR013748
- SMART:   SM00382

Pfam domain/function: PF00004 AAA; PF08542 Rep_fac_C; SSF48019 Pol_clamp_load_C

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 37390; Mature: 37259

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00039 DEAD_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEEEILWAEKYRPRSLDDIVNQKDIVERLKRFVKDKNMPHLLFSGPPGTGKTTAALAL
CCCHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
VHDLYGDNYRQYFLELNASDERGIDVIRNKVKEFARTVAGGNVPFKVVLLDEADNMTADA
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHH
QQALRRTMELYTETTRFILACNYLSKIIEPIQSRTALFRFYPLKKEDVVARLAYIAKNEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
VEYDQKALETIYDITQGDMRKAINILQASSVYGKVTVEAVYKVLGLAQPKEIREMIMLAL
CHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QGNFLKAREKLRELLVNYGLSGEDIIKQIHREVTGNEINIPDDLKVLLVDYIGEVEYRIM
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
EGADDEIQLNALLAKLAVYGEKYLKGK
CCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLEEEILWAEKYRPRSLDDIVNQKDIVERLKRFVKDKNMPHLLFSGPPGTGKTTAALAL
CCHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
VHDLYGDNYRQYFLELNASDERGIDVIRNKVKEFARTVAGGNVPFKVVLLDEADNMTADA
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHH
QQALRRTMELYTETTRFILACNYLSKIIEPIQSRTALFRFYPLKKEDVVARLAYIAKNEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
VEYDQKALETIYDITQGDMRKAINILQASSVYGKVTVEAVYKVLGLAQPKEIREMIMLAL
CHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QGNFLKAREKLRELLVNYGLSGEDIIKQIHREVTGNEINIPDDLKVLLVDYIGEVEYRIM
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
EGADDEIQLNALLAKLAVYGEKYLKGK
CCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479