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The map label for this gene is rfcL [H]

Identifier: 15920689

GI number: 15920689

Start: 474974

End: 476215

Strand: Reverse

Name: rfcL [H]

Synonym: ST0473

Alternate gene names: 15920689

Gene position: 476215-474974 (Counterclockwise)

Preceding gene: 15920690

Following gene: 15920685

Centisome position: 17.67

GC content: 33.49

Gene sequence:

>1242_bases
GTGGAATGGATAGAAAGTTGGTTAAAGGGAAAACCTAATTATAAGGCCGTATTGCTCTATGGACCTCCCGGAGTTGGAAA
GACAACGTTAGCTGAAGCACTAGCTAGAGATTATAAACTCGAATTATTTGAAATGAATGCTAGTGATTCAAGAAATTTAA
ATGATATCAGAACTATGGCTGAACGAGCTTCTATAACAGGGACAATCTTCGGGATTAAGGGAAAATTAATACTTTTAGAT
GAGGTTGATGGTTTGAATGCAAGAGCTGATGCTGGTGCAATTGATGCTATTCTAGAACTTATTAATAAAACTAAATACCC
AATAATTTTAACAGCTAACGATCCTTGGGACCCTTCCTTAAGACCTTTAAGAAATGCTGTAAAAATGATAGAATTAAAAA
GGTTAACTAAATACCCATTAAAGAGAATACTTAAGAAAATATGTGAAGCAGAAAAGATTACATGTGAGGATGAAGCATTA
GATTTTATAATTGAACAGTCAGAGGGAGACGCTAGATATGCTATAAACATGTTACAAGGGGTTGCAGAAGGTTATGGTAG
AGTAACATTAGATATGGCTAAAAATTTAGTTAGGAGAAAAGATAGAGAATTAGATCCTTTTGAAGCATTAAGAGGTGTAT
TTTGGGCTAAATATTACTGGCAAGCTAAAAGCGCAGTAACAGATACTCAGATAGATTACGAACTTTTAATGAGATGGTTA
GATGAAAATATACCATTGCAATATGATAATTTAGAAGATGTATGGAGAGCATATGATGCTTTATCAAGAGCTTCATTATT
TTTAACAAGATCTAAATTAGTTGGGTGGGATCTTTTATCCTATACGTTTGATCTAATGGGGCCTGGTATAGCTTTTGCCT
CCTTAGAGAAGAAAAAGCCTACATATAAGGCTAAGTGGGTCAAGTATCAGTTTCCTCAGTACATTCAACAGTTAGCTAAA
ACTAAAGAAATTAGAGACGCTTTAGATACATTACTAAGAAAGATAGGCCAGGCTATACATGCCTCAAAAGATAAAACTCT
TAATGATTTTCTACCAGCTTTTATAATATATTACAGAAAATACCAGGAAAAACTAGATAAGGAGCTCGAACTTACCGAAA
AAGAAAAAGATGTTATTAAGTTAATTTCTTCATTCTACGAAGGAAGTAAAGTGGAAATAGAAGAACCAGAGAAAAAAGAA
CCAAGTAAAAGAAGAACTACTTCATATCGTCGTAAGTCTTAA

Upstream 100 bases:

>100_bases
TACTTAAAAGGGAAGTAAATTGCCACTACAATGGTTTCTAAAATATAGGCCAAAGACTCTAAATGAAGTAGAGAACGAAG
AAGATGCTAAAAAAGAACTA

Downstream 100 bases:

>100_bases
TTTTATTAATAACCCTAATTGACTTTATCTCAGTATAAGGATACTGTCCATTTTCATCTTTTTCATACTTTTTAACCATG
TCCCAAATAGTAAGAAGAGC

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MEWIESWLKGKPNYKAVLLYGPPGVGKTTLAEALARDYKLELFEMNASDSRNLNDIRTMAERASITGTIFGIKGKLILLD
EVDGLNARADAGAIDAILELINKTKYPIILTANDPWDPSLRPLRNAVKMIELKRLTKYPLKRILKKICEAEKITCEDEAL
DFIIEQSEGDARYAINMLQGVAEGYGRVTLDMAKNLVRRKDRELDPFEALRGVFWAKYYWQAKSAVTDTQIDYELLMRWL
DENIPLQYDNLEDVWRAYDALSRASLFLTRSKLVGWDLLSYTFDLMGPGIAFASLEKKKPTYKAKWVKYQFPQYIQQLAK
TKEIRDALDTLLRKIGQAIHASKDKTLNDFLPAFIIYYRKYQEKLDKELELTEKEKDVIKLISSFYEGSKVEIEEPEKKE
PSKRRTTSYRRKS

Sequences:

>Translated_413_residues
MEWIESWLKGKPNYKAVLLYGPPGVGKTTLAEALARDYKLELFEMNASDSRNLNDIRTMAERASITGTIFGIKGKLILLD
EVDGLNARADAGAIDAILELINKTKYPIILTANDPWDPSLRPLRNAVKMIELKRLTKYPLKRILKKICEAEKITCEDEAL
DFIIEQSEGDARYAINMLQGVAEGYGRVTLDMAKNLVRRKDRELDPFEALRGVFWAKYYWQAKSAVTDTQIDYELLMRWL
DENIPLQYDNLEDVWRAYDALSRASLFLTRSKLVGWDLLSYTFDLMGPGIAFASLEKKKPTYKAKWVKYQFPQYIQQLAK
TKEIRDALDTLLRKIGQAIHASKDKTLNDFLPAFIIYYRKYQEKLDKELELTEKEKDVIKLISSFYEGSKVEIEEPEKKE
PSKRRTTSYRRKS
>Mature_413_residues
MEWIESWLKGKPNYKAVLLYGPPGVGKTTLAEALARDYKLELFEMNASDSRNLNDIRTMAERASITGTIFGIKGKLILLD
EVDGLNARADAGAIDAILELINKTKYPIILTANDPWDPSLRPLRNAVKMIELKRLTKYPLKRILKKICEAEKITCEDEAL
DFIIEQSEGDARYAINMLQGVAEGYGRVTLDMAKNLVRRKDRELDPFEALRGVFWAKYYWQAKSAVTDTQIDYELLMRWL
DENIPLQYDNLEDVWRAYDALSRASLFLTRSKLVGWDLLSYTFDLMGPGIAFASLEKKKPTYKAKWVKYQFPQYIQQLAK
TKEIRDALDTLLRKIGQAIHASKDKTLNDFLPAFIIYYRKYQEKLDKELELTEKEKDVIKLISSFYEGSKVEIEEPEKKE
PSKRRTTSYRRKS

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=246, Percent_Identity=28.8617886178862, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI27501458, Length=209, Percent_Identity=29.6650717703349, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI6677723, Length=179, Percent_Identity=29.608938547486, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI194306571, Length=179, Percent_Identity=29.608938547486, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI194306567, Length=179, Percent_Identity=29.608938547486, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI31563534, Length=187, Percent_Identity=28.8770053475936, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4506491, Length=224, Percent_Identity=27.2321428571429, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI31881687, Length=224, Percent_Identity=27.2321428571429, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17563226, Length=398, Percent_Identity=25.6281407035176, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17541368, Length=381, Percent_Identity=26.246719160105, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17541988, Length=179, Percent_Identity=30.1675977653631, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17554730, Length=195, Percent_Identity=28.7179487179487, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324791, Length=200, Percent_Identity=30.5, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323724, Length=201, Percent_Identity=27.8606965174129, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324478, Length=226, Percent_Identity=28.3185840707965, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324039, Length=180, Percent_Identity=30, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI62472391, Length=296, Percent_Identity=28.7162162162162, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17737765, Length=296, Percent_Identity=28.7162162162162, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28574716, Length=225, Percent_Identity=30.6666666666667, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17647857, Length=205, Percent_Identity=28.780487804878, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921076, Length=181, Percent_Identity=28.7292817679558, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18859927, Length=182, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: NA

Molecular weight: Translated: 47817; Mature: 47817

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWIESWLKGKPNYKAVLLYGPPGVGKTTLAEALARDYKLELFEMNASDSRNLNDIRTMA
CCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
ERASITGTIFGIKGKLILLDEVDGLNARADAGAIDAILELINKTKYPIILTANDPWDPSL
HHHCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCH
RPLRNAVKMIELKRLTKYPLKRILKKICEAEKITCEDEALDFIIEQSEGDARYAINMLQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
VAEGYGRVTLDMAKNLVRRKDRELDPFEALRGVFWAKYYWQAKSAVTDTQIDYELLMRWL
HHHHCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DENIPLQYDNLEDVWRAYDALSRASLFLTRSKLVGWDLLSYTFDLMGPGIAFASLEKKKP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
TYKAKWVKYQFPQYIQQLAKTKEIRDALDTLLRKIGQAIHASKDKTLNDFLPAFIIYYRK
CCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YQEKLDKELELTEKEKDVIKLISSFYEGSKVEIEEPEKKEPSKRRTTSYRRKS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MEWIESWLKGKPNYKAVLLYGPPGVGKTTLAEALARDYKLELFEMNASDSRNLNDIRTMA
CCHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
ERASITGTIFGIKGKLILLDEVDGLNARADAGAIDAILELINKTKYPIILTANDPWDPSL
HHHCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCH
RPLRNAVKMIELKRLTKYPLKRILKKICEAEKITCEDEALDFIIEQSEGDARYAINMLQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
VAEGYGRVTLDMAKNLVRRKDRELDPFEALRGVFWAKYYWQAKSAVTDTQIDYELLMRWL
HHHHCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DENIPLQYDNLEDVWRAYDALSRASLFLTRSKLVGWDLLSYTFDLMGPGIAFASLEKKKP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
TYKAKWVKYQFPQYIQQLAKTKEIRDALDTLLRKIGQAIHASKDKTLNDFLPAFIIYYRK
CCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YQEKLDKELELTEKEKDVIKLISSFYEGSKVEIEEPEKKEPSKRRTTSYRRKS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]