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The map label for this gene is lipB [H]

Identifier: 15893280

GI number: 15893280

Start: 1254837

End: 1255466

Strand: Reverse

Name: lipB [H]

Synonym: RC1357

Alternate gene names: 15893280

Gene position: 1255466-1254837 (Counterclockwise)

Preceding gene: 15893281

Following gene: 15893279

Centisome position: 98.95

GC content: 28.25

Gene sequence:

>630_bases
ATGATAAGATTTATAACTATACCGGATTTTGCGGATTATCAAGTTACTTTGAAATTAATGGAAGATTACGTTAACAAAGT
AATTAGTGATCATAAGCCGGAAATTATTTATTTAGTTGAACATTCGGAAGTATATACAGCCGGTACTAATTATAAACAAG
AAGAATTATTAAATTATGGTGATATTCCGGTAATTTACACAGGACGTGGCGGTAAATTTACTTTTCATGGACCGGGTCAG
CGTGTTATTTATCCAATTCTTAATTTAGCTTCACCAAATCGACACAAAGATTTAAAGCTATATATAAAAATGCTTGAAGA
ATGGATTATAAATAGCTTAAATTATTTTGGAATTAAAGCATATATTATTAAAGATAAAGTAGGAATTTGGGTAAAAGTAA
GGAAAGATGAATTTGCTAAAATTGCTGCAATAGGTGTTAGAGTAAGAAAATGGGTTACATATCACGGTGTAGCCATAAAT
ATTTCAACAGATTTAAGTAAGTTTAGTGGGATTATTCCTTGTGGACTTGAAAATTCTTTAGTAACATCTTTAAATCAGTT
AGGGATTCATGTTGAAATGTCTGAATTTGATAAAATTATTCAAACTGAATTTAATAAAATATTTAAATGA

Upstream 100 bases:

>100_bases
TTCAAATGCATTTTCGTCCTAGTAATTTTTCGGGCGATTTGGATGCTGAAACCATAGCAATTTTAGATGCATTAATATTG
AAATATAAATCTGAATTAAT

Downstream 100 bases:

>100_bases
TCAGAAAATTTTTACTCACAATTTTTGCTCTTTGGGTCGGTGGTTTTGGTTATTATTTATATTTAATAAATTCTTATAAG
CTTAATAGTAATACCACTAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIRFITIPDFADYQVTLKLMEDYVNKVISDHKPEIIYLVEHSEVYTAGTNYKQEELLNYGDIPVIYTGRGGKFTFHGPGQ
RVIYPILNLASPNRHKDLKLYIKMLEEWIINSLNYFGIKAYIIKDKVGIWVKVRKDEFAKIAAIGVRVRKWVTYHGVAIN
ISTDLSKFSGIIPCGLENSLVTSLNQLGIHVEMSEFDKIIQTEFNKIFK

Sequences:

>Translated_209_residues
MIRFITIPDFADYQVTLKLMEDYVNKVISDHKPEIIYLVEHSEVYTAGTNYKQEELLNYGDIPVIYTGRGGKFTFHGPGQ
RVIYPILNLASPNRHKDLKLYIKMLEEWIINSLNYFGIKAYIIKDKVGIWVKVRKDEFAKIAAIGVRVRKWVTYHGVAIN
ISTDLSKFSGIIPCGLENSLVTSLNQLGIHVEMSEFDKIIQTEFNKIFK
>Mature_209_residues
MIRFITIPDFADYQVTLKLMEDYVNKVISDHKPEIIYLVEHSEVYTAGTNYKQEELLNYGDIPVIYTGRGGKFTFHGPGQ
RVIYPILNLASPNRHKDLKLYIKMLEEWIINSLNYFGIKAYIIKDKVGIWVKVRKDEFAKIAAIGVRVRKWVTYHGVAIN
ISTDLSKFSGIIPCGLENSLVTSLNQLGIHVEMSEFDKIIQTEFNKIFK

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=174, Percent_Identity=36.7816091954023, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI87081767, Length=151, Percent_Identity=39.7350993377483, Blast_Score=120, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323268, Length=186, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355483, Length=182, Percent_Identity=36.2637362637363, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24092; Mature: 24092

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFITIPDFADYQVTLKLMEDYVNKVISDHKPEIIYLVEHSEVYTAGTNYKQEELLNYG
CEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCHHHHCCCC
DIPVIYTGRGGKFTFHGPGQRVIYPILNLASPNRHKDLKLYIKMLEEWIINSLNYFGIKA
CCCEEEECCCCEEEEECCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
YIIKDKVGIWVKVRKDEFAKIAAIGVRVRKWVTYHGVAINISTDLSKFSGIIPCGLENSL
EEEECCCEEEEEECCCCHHHHHHHHHHHHEEEEECCEEEEEECCHHHHCCCCCCCCCHHH
VTSLNQLGIHVEMSEFDKIIQTEFNKIFK
HHHHHHCCEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRFITIPDFADYQVTLKLMEDYVNKVISDHKPEIIYLVEHSEVYTAGTNYKQEELLNYG
CEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCHHHHCCCC
DIPVIYTGRGGKFTFHGPGQRVIYPILNLASPNRHKDLKLYIKMLEEWIINSLNYFGIKA
CCCEEEECCCCEEEEECCCCEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
YIIKDKVGIWVKVRKDEFAKIAAIGVRVRKWVTYHGVAINISTDLSKFSGIIPCGLENSL
EEEECCCEEEEEECCCCHHHHHHHHHHHHEEEEECCEEEEEECCHHHHCCCCCCCCCHHH
VTSLNQLGIHVEMSEFDKIIQTEFNKIFK
HHHHHHCCEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA