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The map label for this gene is ampD [H]

Identifier: 15893281

GI number: 15893281

Start: 1255463

End: 1256206

Strand: Reverse

Name: ampD [H]

Synonym: RC1358

Alternate gene names: 15893281

Gene position: 1256206-1255463 (Counterclockwise)

Preceding gene: 15893282

Following gene: 15893280

Centisome position: 99.01

GC content: 32.8

Gene sequence:

>744_bases
ATGATAGTAATCGATAAAACATCTTATAGAGCTGTGGGATTTGATAAACGTATTAAATTCCTAGTGCTACATTATACACA
GTGTGATTTTAAGCAGTCTTTAGATTTTCTAACCGGTGAAAAGTTAAGTAGCCATTATTTAATAAACAATGAAAACCCTG
AACATATATTTCAATTAGTAGAAGAGCGTGATAGAGCATGGCATGCTGGAGTAAGTTACTGGCAAGGTCATGAGCGTATT
AATGATACATCAATCGGTATTGAAATCGTAAATCCTGCTTTTGAAGTAAATGCAAAAAACAATGATATAGTTTGGCTACC
CTATTCGGAAAGGCAAATTAATAGTGTTATAAGTTTGTGTAAACAGATTATTGCTAGATATGATATTAAACCTACTAGAG
TAGTTGGGCATTCTGATATTGCTCCAGGTAGAAAACAGGACCCTGGCCCGCTATTTCCTTGGAAACTATTATATGATAAC
GATATTGGAGCTTGGTATGATGAACAGGTGTTTAACGATCTATTGCCGCAAGTCGACATAACCGATATTGCAGTAATTCA
GCAAAAATTTATTACATACGGTTATAAACTTGAAGTTACCGAAATTTTAGACTCTAAAATGAAAGATATTATAATTTCGT
TTCAAATGCATTTTCGTCCTAGTAATTTTTCGGGCGATTTGGATGCTGAAACCATAGCAATTTTAGATGCATTAATATTG
AAATATAAATCTGAATTAATATGA

Upstream 100 bases:

>100_bases
TCTTCTCGCAATGACGATTTACCGATCCATGCAAGCAAGCTTTCACAAGAATTATATAGAAAAAAACATACAATAGTGAC
ATCCTTTAAAGTTTTTATAT

Downstream 100 bases:

>100_bases
TAAGATTTATAACTATACCGGATTTTGCGGATTATCAAGTTACTTTGAAATTAATGGAAGATTACGTTAACAAAGTAATT
AGTGATCATAAGCCGGAAAT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MIVIDKTSYRAVGFDKRIKFLVLHYTQCDFKQSLDFLTGEKLSSHYLINNENPEHIFQLVEERDRAWHAGVSYWQGHERI
NDTSIGIEIVNPAFEVNAKNNDIVWLPYSERQINSVISLCKQIIARYDIKPTRVVGHSDIAPGRKQDPGPLFPWKLLYDN
DIGAWYDEQVFNDLLPQVDITDIAVIQQKFITYGYKLEVTEILDSKMKDIIISFQMHFRPSNFSGDLDAETIAILDALIL
KYKSELI

Sequences:

>Translated_247_residues
MIVIDKTSYRAVGFDKRIKFLVLHYTQCDFKQSLDFLTGEKLSSHYLINNENPEHIFQLVEERDRAWHAGVSYWQGHERI
NDTSIGIEIVNPAFEVNAKNNDIVWLPYSERQINSVISLCKQIIARYDIKPTRVVGHSDIAPGRKQDPGPLFPWKLLYDN
DIGAWYDEQVFNDLLPQVDITDIAVIQQKFITYGYKLEVTEILDSKMKDIIISFQMHFRPSNFSGDLDAETIAILDALIL
KYKSELI
>Mature_247_residues
MIVIDKTSYRAVGFDKRIKFLVLHYTQCDFKQSLDFLTGEKLSSHYLINNENPEHIFQLVEERDRAWHAGVSYWQGHERI
NDTSIGIEIVNPAFEVNAKNNDIVWLPYSERQINSVISLCKQIIARYDIKPTRVVGHSDIAPGRKQDPGPLFPWKLLYDN
DIGAWYDEQVFNDLLPQVDITDIAVIQQKFITYGYKLEVTEILDSKMKDIIISFQMHFRPSNFSGDLDAETIAILDALIL
KYKSELI

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=245, Percent_Identity=45.3061224489796, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1786300, Length=114, Percent_Identity=40.3508771929825, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 28627; Mature: 28627

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIDKTSYRAVGFDKRIKFLVLHYTQCDFKQSLDFLTGEKLSSHYLINNENPEHIFQLV
CEEECCCCCEECCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHH
EERDRAWHAGVSYWQGHERINDTSIGIEIVNPAFEVNAKNNDIVWLPYSERQINSVISLC
HHHHHHHHHCCHHHCCCCCCCCCEEEEEEECCEEEECCCCCCEEEEECCHHHHHHHHHHH
KQIIARYDIKPTRVVGHSDIAPGRKQDPGPLFPWKLLYDNDIGAWYDEQVFNDLLPQVDI
HHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCH
TDIAVIQQKFITYGYKLEVTEILDSKMKDIIISFQMHFRPSNFSGDLDAETIAILDALIL
HHHHHHHHHHHHCCCEEEHHHHHHHHHHHEEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
KYKSELI
HHHHHCC
>Mature Secondary Structure
MIVIDKTSYRAVGFDKRIKFLVLHYTQCDFKQSLDFLTGEKLSSHYLINNENPEHIFQLV
CEEECCCCCEECCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHH
EERDRAWHAGVSYWQGHERINDTSIGIEIVNPAFEVNAKNNDIVWLPYSERQINSVISLC
HHHHHHHHHCCHHHCCCCCCCCCEEEEEEECCEEEECCCCCCEEEEECCHHHHHHHHHHH
KQIIARYDIKPTRVVGHSDIAPGRKQDPGPLFPWKLLYDNDIGAWYDEQVFNDLLPQVDI
HHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCH
TDIAVIQQKFITYGYKLEVTEILDSKMKDIIISFQMHFRPSNFSGDLDAETIAILDALIL
HHHHHHHHHHHHCCCEEEHHHHHHHHHHHEEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
KYKSELI
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]