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The map label for this gene is pdhC

Identifier: 15892687

GI number: 15892687

Start: 728147

End: 729385

Strand: Reverse

Name: pdhC

Synonym: RC0764

Alternate gene names: 15892687

Gene position: 729385-728147 (Counterclockwise)

Preceding gene: 15892688

Following gene: 15892685

Centisome position: 57.49

GC content: 35.43

Gene sequence:

>1239_bases
ATGCCGATTAAAATTTTAATGCCTGCTTTATCTCCAACTATGACGGAAGGAAATCTAGCTAGGTGGTTAAAAAAAGAAGG
GGACAAAGTTAATCCTGGAGAAGTAATTGCTGAAATTGAAACCGATAAAGCAACGATGGAAGTAGAAGCAGTAGATGAAG
GTATACTTGCCAAAATAGTTATCCCACAAAATAGTCAAAATGTACCTGTTAACTCTTTAATTGCCGTGTTAAGTGAAGAG
GGAGAAGAAAAAACAGACATTGATGCGTTTATTGCAAAAAATAATAGTGTATCACCGTCACCGAAAACAGATGCTAATCT
TCCGAAACCCCACGAGAATATAGCTAATGTAGAAGAACAGGTAACAGTTATAAAACATGATGTTAGTAGAATATTCGCCT
CACCGCTTGCAAAAAGACTTGCAAAAATGAGAAATATTAGATTTGAGAGCGTGAAAGGTAGCGGTCCGCACGGTAGAATA
GTCAAACAAGATATCTTATCATATACTCCTAGCACTGCTCATAATAAAATAGTTAGCAGAAATCCTGAAGAATATCGTTT
AGTACCAAATAATAATATCCGCAAGATTATAGCCAAGCGTCTGCTTGAATCTAAACAAACAGTTCCACATTTTTATTTAT
CTATAGAATGTAATGTCGATAAATTGTTAGATATAAGAGAAGACATTAATAAATTTTTTTCTGAAGATAAATCAACAAGG
ATATCGGTTAATGATTTTATTATTTTAGCAGTAGCCAAAGCTTTACAAGAAGTGCCAAATGCCAATGCTAGCTGGGGAGA
AGATGCAATTAGATATTACAATAATGTCGATATTTCAGTAGCGGTAGCGATTGAGAACGGGCTTGTTACACCGATAGTTA
AAAATGCCAATCAAAAAAACATTCTAGAACTATCTCGTGAAATGAAAGCATTAATAAAGAAAGCAAAAGATAATAAATTA
ACCCCTGAAGAATTTCAGGGCGGAGGGTTTACCATTTCTAACCTCGGTATGTACGGTATTAAGAATTTCAATGCTATAAT
TAACCCACCACAAAGTTGTATAATGGGCGTCGGAGCTAGTGCAAAACGTGCTATAGTCAAGAATGATCAAATAACTATAG
CAACAATTATGGACGTAACTCTTTCTGCCGATCACCGAGTAGTAGACGGAGCAGTCGGTGCTGAGTTCTTGGTAGCATTT
AAAAAGTTTATAGAGAGTCCCGTTTTGATGTTGATATAA

Upstream 100 bases:

>100_bases
TCAGGAATTTTACTGTTTCATGAGGTTGCCGCGTCGAGGCTTGGCCTCTCCTCGCAATGACGTGTAATAACAACAATAAA
AAGTCAAATCTAAATAAGTT

Downstream 100 bases:

>100_bases
GTCGTCATTACGAGCAGCCATAGGCTGGCTGCGTGACAATCTCGTCAAATATCCTGAGATTGCTTCATCGCAGCTTTCAG
TTGCTTCTCGCAATAACGTT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPIKILMPALSPTMTEGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIVIPQNSQNVPVNSLIAVLSEE
GEEKTDIDAFIAKNNSVSPSPKTDANLPKPHENIANVEEQVTVIKHDVSRIFASPLAKRLAKMRNIRFESVKGSGPHGRI
VKQDILSYTPSTAHNKIVSRNPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKFFSEDKSTR
ISVNDFIILAVAKALQEVPNANASWGEDAIRYYNNVDISVAVAIENGLVTPIVKNANQKNILELSREMKALIKKAKDNKL
TPEEFQGGGFTISNLGMYGIKNFNAIINPPQSCIMGVGASAKRAIVKNDQITIATIMDVTLSADHRVVDGAVGAEFLVAF
KKFIESPVLMLI

Sequences:

>Translated_412_residues
MPIKILMPALSPTMTEGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIVIPQNSQNVPVNSLIAVLSEE
GEEKTDIDAFIAKNNSVSPSPKTDANLPKPHENIANVEEQVTVIKHDVSRIFASPLAKRLAKMRNIRFESVKGSGPHGRI
VKQDILSYTPSTAHNKIVSRNPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKFFSEDKSTR
ISVNDFIILAVAKALQEVPNANASWGEDAIRYYNNVDISVAVAIENGLVTPIVKNANQKNILELSREMKALIKKAKDNKL
TPEEFQGGGFTISNLGMYGIKNFNAIINPPQSCIMGVGASAKRAIVKNDQITIATIMDVTLSADHRVVDGAVGAEFLVAF
KKFIESPVLMLI
>Mature_411_residues
PIKILMPALSPTMTEGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIVIPQNSQNVPVNSLIAVLSEEG
EEKTDIDAFIAKNNSVSPSPKTDANLPKPHENIANVEEQVTVIKHDVSRIFASPLAKRLAKMRNIRFESVKGSGPHGRIV
KQDILSYTPSTAHNKIVSRNPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKFFSEDKSTRI
SVNDFIILAVAKALQEVPNANASWGEDAIRYYNNVDISVAVAIENGLVTPIVKNANQKNILELSREMKALIKKAKDNKLT
PEEFQGGGFTISNLGMYGIKNFNAIINPPQSCIMGVGASAKRAIVKNDQITIATIMDVTLSADHRVVDGAVGAEFLVAFK
KFIESPVLMLI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI31711992, Length=439, Percent_Identity=42.8246013667426, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=38.9130434782609, Blast_Score=293, Evalue=1e-79,
Organism=Homo sapiens, GI203098753, Length=460, Percent_Identity=38.9130434782609, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=45.5089820359281, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI110671329, Length=428, Percent_Identity=27.5700934579439, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI19923748, Length=224, Percent_Identity=33.0357142857143, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=32, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1786305, Length=395, Percent_Identity=30.8860759493671, Blast_Score=163, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=434, Percent_Identity=41.2442396313364, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=34.8534201954397, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI25146366, Length=424, Percent_Identity=31.3679245283019, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=434, Percent_Identity=26.9585253456221, Blast_Score=155, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=40.0437636761488, Blast_Score=305, Evalue=8e-84,
Organism=Saccharomyces cerevisiae, GI6320352, Length=409, Percent_Identity=30.3178484107579, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6321632, Length=216, Percent_Identity=30.5555555555556, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582497, Length=292, Percent_Identity=46.5753424657534, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI20129315, Length=292, Percent_Identity=46.5753424657534, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI18859875, Length=424, Percent_Identity=26.6509433962264, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=31.8181818181818, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ODP2_RICCN (Q92HK7)

Other databases:

- EMBL:   AE006914
- PIR:   D97795
- RefSeq:   NP_360401.1
- ProteinModelPortal:   Q92HK7
- GeneID:   927481
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC0764
- NMPDR:   fig|272944.1.peg.764
- OMA:   INSADEN
- ProtClustDB:   PRK11856
- BioCyc:   RCON272944:RC0764-MONOMER
- BRENDA:   2.3.1.12
- GO:   GO:0006096
- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- Gene3D:   G3DSA:4.10.320.10
- Gene3D:   G3DSA:3.30.559.10
- TIGRFAMs:   TIGR01349

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding; SSF47005 E3_bd; SSF51230 Hybrid_motif

EC number: =2.3.1.12

Molecular weight: Translated: 45427; Mature: 45296

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: ACT_SITE 385-385

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIKILMPALSPTMTEGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIV
CCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IPQNSQNVPVNSLIAVLSEEGEEKTDIDAFIAKNNSVSPSPKTDANLPKPHENIANVEEQ
ECCCCCCCCHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
VTVIKHDVSRIFASPLAKRLAKMRNIRFESVKGSGPHGRIVKQDILSYTPSTAHNKIVSR
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCCHHHCHHHCC
NPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKFFSEDKSTR
CCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCE
ISVNDFIILAVAKALQEVPNANASWGEDAIRYYNNVDISVAVAIENGLVTPIVKNANQKN
EEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCHHH
ILELSREMKALIKKAKDNKLTPEEFQGGGFTISNLGMYGIKNFNAIINPPQSCIMGVGAS
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHCCCCC
AKRAIVKNDQITIATIMDVTLSADHRVVDGAVGAEFLVAFKKFIESPVLMLI
CCHHEECCCCEEEEEEEEEEECCCCEEECCHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
PIKILMPALSPTMTEGNLARWLKKEGDKVNPGEVIAEIETDKATMEVEAVDEGILAKIV
CCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IPQNSQNVPVNSLIAVLSEEGEEKTDIDAFIAKNNSVSPSPKTDANLPKPHENIANVEEQ
ECCCCCCCCHHHHHHHHHHCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
VTVIKHDVSRIFASPLAKRLAKMRNIRFESVKGSGPHGRIVKQDILSYTPSTAHNKIVSR
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCCCCHHHCHHHCC
NPEEYRLVPNNNIRKIIAKRLLESKQTVPHFYLSIECNVDKLLDIREDINKFFSEDKSTR
CCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCE
ISVNDFIILAVAKALQEVPNANASWGEDAIRYYNNVDISVAVAIENGLVTPIVKNANQKN
EEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCHHH
ILELSREMKALIKKAKDNKLTPEEFQGGGFTISNLGMYGIKNFNAIINPPQSCIMGVGAS
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHCCCCC
AKRAIVKNDQITIATIMDVTLSADHRVVDGAVGAEFLVAFKKFIESPVLMLI
CCHHEECCCCEEEEEEEEEEECCCCEEECCHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893