Click here to switch to the map view.

The map label for this gene is prfA [H]

Identifier: 15892685

GI number: 15892685

Start: 726943

End: 728010

Strand: Reverse

Name: prfA [H]

Synonym: RC0762

Alternate gene names: 15892685

Gene position: 728010-726943 (Counterclockwise)

Preceding gene: 15892687

Following gene: 15892684

Centisome position: 57.38

GC content: 37.08

Gene sequence:

>1068_bases
ATGCGTTTTTCAGATAATTTAGCAAAAATTTTAGACAAATACGAAAATTTAGGCAACAAACTATCTTCCGGCATTATGGG
AGACGAGTTTGTTAAGGCATCTAAAGAATATGCAGAACTTGAAGATGTTGTAGCAAAAATTAAAGAATATAATAAGGCTA
AATCTGAGCTTGAAGAAGCAAATAATTTTAAGCTAGAAGTGGGACTTGATAATGCCACTTTAGAAATGATTGAAGACGAA
ATATACACTCTTGAAAATTCGTTACCAAAACTTGAAAGAGCCGTAAAAATTGCTTTATTACCAAAAGATGATGCGGATAG
TAAAAGTGCTATTATTGAAGTTAGAGCAGGAAGCGGCGGAGAAGAAGCGGCACTTTTTGCTGCCGTACTTTTTAATATGT
ATCAAAGATATGCTGAGTTAAAAGGATGGCATTTTGAGATATTAGCAATTTCCGATACGGGAATAGGCGGTTATAAAGAA
GCATCGGCGTCAATAAAAGGCAAAGATGTATTCTCTAAGCTCAAATTTGAGTCAGGCGTTCATAGAGTACAGAGAGTACC
GGAAACGGAATCACAAGGACGTATTCATACTTCGGCAGCAACGGTTGCAGTACTTCCTGAGGCTGAAGAGGTTGATATTA
AAATTGAAGATAAAGACTTAAGAATCGATACTTATAGAGCTTCAGGGGCTGGTGGACAGCACGTTAATACTACTGATTCT
GCCGTACGTATAACTCATATCCCTACCGGTATTACCGTAGCCTTGCAAGATGAGAAGTCACAGCATAAAAATAAAGCAAA
GGCGTTAAAAATTCTACGTGCTAGAATTTATGAAGAAGAACGACGTAAAAAGGAACAAGAAAGAGCCGATAGCAGAAGAG
GGCAGGTAGGTTCGGGAGATCGTTCGGAACGGATACGTACTTATAATTTCCCACAGGGAAGAGTATCCGACCATAGAATA
AACTTAACACTTTATAAGATAGACGAAGTAGTTAAAAATGGACAATTAGATGAGTTTGTTGAAGCTTTAATTGCAGATGA
TGAAGCTAAAAAACTTTTGGAGATATAA

Upstream 100 bases:

>100_bases
CAATCTCGTCAAATATCCTGAGATTGCTTCATCGCAGCTTTCAGTTGCTTCTCGCAATAACGTTGAAATCTCACCCATAA
ACAAATCACAATAAAATAAC

Downstream 100 bases:

>100_bases
GTGTATATCGTTCACCTCTGTTCACCCCGTGGCTTGACCACGGGGTGAACAGAGGTGAAATTGATCCACGCAACAATGCC
TACTCACAATGACGATATAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MRFSDNLAKILDKYENLGNKLSSGIMGDEFVKASKEYAELEDVVAKIKEYNKAKSELEEANNFKLEVGLDNATLEMIEDE
IYTLENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGGEEAALFAAVLFNMYQRYAELKGWHFEILAISDTGIGGYKE
ASASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRKKEQERADSRRGQVGSGDRSERIRTYNFPQGRVSDHRI
NLTLYKIDEVVKNGQLDEFVEALIADDEAKKLLEI

Sequences:

>Translated_355_residues
MRFSDNLAKILDKYENLGNKLSSGIMGDEFVKASKEYAELEDVVAKIKEYNKAKSELEEANNFKLEVGLDNATLEMIEDE
IYTLENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGGEEAALFAAVLFNMYQRYAELKGWHFEILAISDTGIGGYKE
ASASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRKKEQERADSRRGQVGSGDRSERIRTYNFPQGRVSDHRI
NLTLYKIDEVVKNGQLDEFVEALIADDEAKKLLEI
>Mature_355_residues
MRFSDNLAKILDKYENLGNKLSSGIMGDEFVKASKEYAELEDVVAKIKEYNKAKSELEEANNFKLEVGLDNATLEMIEDE
IYTLENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGGEEAALFAAVLFNMYQRYAELKGWHFEILAISDTGIGGYKE
ASASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRKKEQERADSRRGQVGSGDRSERIRTYNFPQGRVSDHRI
NLTLYKIDEVVKNGQLDEFVEALIADDEAKKLLEI

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=316, Percent_Identity=47.1518987341772, Blast_Score=292, Evalue=3e-79,
Organism=Homo sapiens, GI34577120, Length=359, Percent_Identity=40.3899721448468, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI166795305, Length=209, Percent_Identity=49.2822966507177, Blast_Score=209, Evalue=2e-54,
Organism=Escherichia coli, GI1787462, Length=325, Percent_Identity=55.0769230769231, Blast_Score=343, Evalue=7e-96,
Organism=Escherichia coli, GI2367172, Length=314, Percent_Identity=38.8535031847134, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=261, Percent_Identity=42.1455938697318, Blast_Score=197, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=372, Percent_Identity=43.010752688172, Blast_Score=243, Evalue=2e-65,
Organism=Drosophila melanogaster, GI19921226, Length=328, Percent_Identity=42.6829268292683, Blast_Score=249, Evalue=3e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39746; Mature: 39746

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSDNLAKILDKYENLGNKLSSGIMGDEFVKASKEYAELEDVVAKIKEYNKAKSELEEA
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNFKLEVGLDNATLEMIEDEIYTLENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGG
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAAVLFNMYQRYAELKGWHFEILAISDTGIGGYKEASASIKGKDVFSKLKFESGV
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHH
HRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRKKEQERADSRRGQVGSGD
EEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RSERIRTYNFPQGRVSDHRINLTLYKIDEVVKNGQLDEFVEALIADDEAKKLLEI
CCCCCEEECCCCCCCCCCEEEEEEEEHHHHHHCCCHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MRFSDNLAKILDKYENLGNKLSSGIMGDEFVKASKEYAELEDVVAKIKEYNKAKSELEEA
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NNFKLEVGLDNATLEMIEDEIYTLENSLPKLERAVKIALLPKDDADSKSAIIEVRAGSGG
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAAVLFNMYQRYAELKGWHFEILAISDTGIGGYKEASASIKGKDVFSKLKFESGV
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHH
HRVQRVPETESQGRIHTSAATVAVLPEAEEVDIKIEDKDLRIDTYRASGAGGQHVNTTDS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCC
AVRITHIPTGITVALQDEKSQHKNKAKALKILRARIYEEERRKKEQERADSRRGQVGSGD
EEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RSERIRTYNFPQGRVSDHRINLTLYKIDEVVKNGQLDEFVEALIADDEAKKLLEI
CCCCCEEECCCCCCCCCCEEEEEEEEHHHHHHCCCHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA