Click here to switch to the map view.

The map label for this gene is rpoH [H]

Identifier: 15892330

GI number: 15892330

Start: 407395

End: 408276

Strand: Reverse

Name: rpoH [H]

Synonym: RC0407

Alternate gene names: 15892330

Gene position: 408276-407395 (Counterclockwise)

Preceding gene: 15892332

Following gene: 15892325

Centisome position: 32.18

GC content: 32.2

Gene sequence:

>882_bases
ATGACTAATAATATTAATGCATTAGCAATTTCTAGCGAATCAGGATTTTATAGCTACTTACAGAAAATCAATAAAATTCC
TTCACTTACGCAAGAAGAAGAATTGTTATTAGCAAAATCCTATTTAGAAGGGAATGATTTACAAGCTGCACATAAATTAG
TAACAAGCCATCTTAAACTTGTTGCAAAAATTGCAAGCGGCTATAGGACTTACGGTCTTCCTATTACCGAATTGGTTTCA
GAAGGTAATATAGGTTTAATGCAGGCAGTAAAAAAATTCAATCCTGAACTTGGTTTTCGCCTATCAACATATGCGATGTG
GTGGATTAAAGCAGCCATACAAGAATATATATTAAAATCTTGGTCATTAGTAAAGATGGGAACAACGGCCGCACAAAAAA
AATTATTTTTTAGTCTTAATAAAGTTAAACATAAAATTACTAATTTATACTCAAGGGCTATTACTACCGATGATTTTGTA
CAAATAGCAGATGAGCTAGGCGTTTCGGTTAATGAAGTTTCTGAAATGAATACTAGAATTTCAGGTCCTGATTTATCGTT
AAATAACTCTATAAATAGTGACGATGCAGAATCCGGAGAATTAATAGAACTATTACCGGAAACACGCCCTACTCCTGAAG
CTATGGCTATTAATAAGCAAAATTCTACCAGTAAAAGGCAGTTACTATCAAATGCTATGAAGATTTTAAACGACAGGGAA
CTGCGTATTTTAACAGAGCGTAAATTAACGGATACTCCTAAAACTCTAGATATTCTAAGCAATGAGTATAATATTTCTAA
GGAACGTATTAGACAAATAGAAAATACGGCTTTTGAAAAAATAAAGAAGTTTATTTTAAACCATAGTAGAGAAGCAGCTT
AA

Upstream 100 bases:

>100_bases
TTTTTTAGACATTATTTGATTTTATTTAAAAATACTCTAGAAAAAGTTTATTTTAGATCATATATGTGTTAATATAAAAA
AAATAGTTTTTAAGGTTAGT

Downstream 100 bases:

>100_bases
GCCTTATTGGACTGTCATCCCGTGGCTTGACCACAGGATCCAAAAACAACTAATAGTATTTTTACTGGATACCGCGATTA
AGTCACGGTATGACAACGGA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTNNINALAISSESGFYSYLQKINKIPSLTQEEELLLAKSYLEGNDLQAAHKLVTSHLKLVAKIASGYRTYGLPITELVS
EGNIGLMQAVKKFNPELGFRLSTYAMWWIKAAIQEYILKSWSLVKMGTTAAQKKLFFSLNKVKHKITNLYSRAITTDDFV
QIADELGVSVNEVSEMNTRISGPDLSLNNSINSDDAESGELIELLPETRPTPEAMAINKQNSTSKRQLLSNAMKILNDRE
LRILTERKLTDTPKTLDILSNEYNISKERIRQIENTAFEKIKKFILNHSREAA

Sequences:

>Translated_293_residues
MTNNINALAISSESGFYSYLQKINKIPSLTQEEELLLAKSYLEGNDLQAAHKLVTSHLKLVAKIASGYRTYGLPITELVS
EGNIGLMQAVKKFNPELGFRLSTYAMWWIKAAIQEYILKSWSLVKMGTTAAQKKLFFSLNKVKHKITNLYSRAITTDDFV
QIADELGVSVNEVSEMNTRISGPDLSLNNSINSDDAESGELIELLPETRPTPEAMAINKQNSTSKRQLLSNAMKILNDRE
LRILTERKLTDTPKTLDILSNEYNISKERIRQIENTAFEKIKKFILNHSREAA
>Mature_292_residues
TNNINALAISSESGFYSYLQKINKIPSLTQEEELLLAKSYLEGNDLQAAHKLVTSHLKLVAKIASGYRTYGLPITELVSE
GNIGLMQAVKKFNPELGFRLSTYAMWWIKAAIQEYILKSWSLVKMGTTAAQKKLFFSLNKVKHKITNLYSRAITTDDFVQ
IADELGVSVNEVSEMNTRISGPDLSLNNSINSDDAESGELIELLPETRPTPEAMAINKQNSTSKRQLLSNAMKILNDREL
RILTERKLTDTPKTLDILSNEYNISKERIRQIENTAFEKIKKFILNHSREAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=287, Percent_Identity=37.6306620209059, Blast_Score=190, Evalue=8e-50,
Organism=Escherichia coli, GI1789098, Length=274, Percent_Identity=30.2919708029197, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1789448, Length=268, Percent_Identity=27.9850746268657, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33045; Mature: 32914

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNINALAISSESGFYSYLQKINKIPSLTQEEELLLAKSYLEGNDLQAAHKLVTSHLKL
CCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VAKIASGYRTYGLPITELVSEGNIGLMQAVKKFNPELGFRLSTYAMWWIKAAIQEYILKS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTAAQKKLFFSLNKVKHKITNLYSRAITTDDFVQIADELGVSVNEVSEMNTRI
CCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCC
SGPDLSLNNSINSDDAESGELIELLPETRPTPEAMAINKQNSTSKRQLLSNAMKILNDRE
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHCCCH
LRILTERKLTDTPKTLDILSNEYNISKERIRQIENTAFEKIKKFILNHSREAA
HEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TNNINALAISSESGFYSYLQKINKIPSLTQEEELLLAKSYLEGNDLQAAHKLVTSHLKL
CCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VAKIASGYRTYGLPITELVSEGNIGLMQAVKKFNPELGFRLSTYAMWWIKAAIQEYILKS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTAAQKKLFFSLNKVKHKITNLYSRAITTDDFVQIADELGVSVNEVSEMNTRI
CCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCC
SGPDLSLNNSINSDDAESGELIELLPETRPTPEAMAINKQNSTSKRQLLSNAMKILNDRE
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHCCCH
LRILTERKLTDTPKTLDILSNEYNISKERIRQIENTAFEKIKKFILNHSREAA
HEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]