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The map label for this gene is pcnA

Identifier: 41410430

GI number: 41410430

Start: 4807753

End: 4809195

Strand: Reverse

Name: pcnA

Synonym: MAP4332c

Alternate gene names: 41410430

Gene position: 4809195-4807753 (Counterclockwise)

Preceding gene: 41410440

Following gene: 41410429

Centisome position: 99.57

GC content: 69.09

Gene sequence:

>1443_bases
GTGCCTGACGCCGCCCAGGATGCCGAGCTGCTGACCGCCGCCGCGGTCGCGCTCAACAAGCATGCGCCGATGCTGCGCGA
GCTGGGTTCTGCGTTCGACGCCGCGGGCCACCAGCTCTACCTCGTCGGCGGTTCGGTGCGCGATGCCCTGCTGGGCCGGT
TGAGCCCCGACCTGGACTTCACCACCGACGCGCGCCCCGAGCAGGTGCAGCAAATCCTGCGGCGGTGGGCCGACAACCTG
TGGGACACCGGAATCGAATTCGGCACCGTCGGCGTCGGCAAGGGCGACCGCCGCCTGGAGATCACCACCTTCCGCGCCGA
CACCTACGACCAGGTGTCCCGAAATCCCCAGGTGCGCTTCGGCAATCGCCTCGAAGACGACCTGGTGCGCCGCGATTTCA
CCGCCAACGCCATGGCGGTGCGGATCACGCCCGACGGACCGGGCGAATTCCTGGACCCCCTGGGCGGATTGGCGGCGCTG
CGCCAGCGGGTGCTGGACACCCCCGCCACACCGGAGGAGTCGTTCGGCGACGATCCGTTGCGGATGCTGCGCGCGGCCCG
GTTCGTGTCGCAACTCGGCTTCACCGTCGCGCCCCGGGTGCGCGAAGCGATCGAGCGGATGGCCCCGCAGCTGGCCCGGA
TCAGCGCGGAACGGGTGGCCGCCGAGCTGGACAAGCTGGTGCTCGGCGACGATCCGGTGGCCGGGATCGATCTGCTGGTG
CACAGCGGAATGGGTGAGGTGGTGCTGCCCGAGATCGGCGGCATGCAGATGGCCATCGACGAACACCACCAGCACAAGGA
CGTCTACCAGCACTCGCTGACCGTGTTGCGTCAGGCGATCGCCCTCGAAGACGACGGCCCGGATCTGGTGCTGCGCTGGG
CCGCGCTGCTGCACGACATCGGCAAGCCCGCCACCCGACGCCACGAGCCCAACGGCGGCGTGAGCTTTCACCACCACGAG
GTGGTCGGCGCCAAGATGGTCCGAAAACGGTTGCGCGCCTTGAAGTATTCCAAGCAGATGATCGACGACGTCTCGCAGCT
GGTGTATCTGCATCTGCGGTTCCACGGCTATGGCGACGGCAAGTGGACCGATTCGGCGGTGCGCCGCTACGTCACCGACG
CCGGGCCGCTGCTGCCCCGGCTGCACAAGCTGGTGCGAGCCGACTGCACGACGCGCAACAAGCGCCGGGCCGCTCGGCTG
CACGCAAGTTATGACCGGCTCGAGGCGCGGATCGCCGAGCTGGCCGCTCAGGAGGATTTGGCGCGGGTCCGCCCCGATCT
GGACGGCAACCAGATCATGGAGATCCTGGGCATCCCGGCCGGCCCTCAGGTCGGGGAGGCGTGGCGGTTCCTGAAGGAGC
TGCGGCTGGAGCGGGGACCGTTGGATCCCGACGAGGCGACCGCAGAACTGCTGTCCTGGTGGCGATCTCGCGGGAACGGG
TAG

Upstream 100 bases:

>100_bases
GTAAGGCTACTGACGCTCCGCGCCGGGTCAATTCACCATTGATCCCGTGCGAGGCTCCTCACACCGGCTTTTGGTGCAGG
TCCGACTAGGCTGATCGAAC

Downstream 100 bases:

>100_bases
CCGCGGCCGCGCGTCCGACAATTTGTGGACTATTGCTTGGCAGGCGACGGCGGCGCGGCCGCGATCTGGCACGGTCAACC
CGACCTCGACCTTGACGGCG

Product: PcnA

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPDAAQDAELLTAAAVALNKHAPMLRELGSAFDAAGHQLYLVGGSVRDALLGRLSPDLDFTTDARPEQVQQILRRWADNL
WDTGIEFGTVGVGKGDRRLEITTFRADTYDQVSRNPQVRFGNRLEDDLVRRDFTANAMAVRITPDGPGEFLDPLGGLAAL
RQRVLDTPATPEESFGDDPLRMLRAARFVSQLGFTVAPRVREAIERMAPQLARISAERVAAELDKLVLGDDPVAGIDLLV
HSGMGEVVLPEIGGMQMAIDEHHQHKDVYQHSLTVLRQAIALEDDGPDLVLRWAALLHDIGKPATRRHEPNGGVSFHHHE
VVGAKMVRKRLRALKYSKQMIDDVSQLVYLHLRFHGYGDGKWTDSAVRRYVTDAGPLLPRLHKLVRADCTTRNKRRAARL
HASYDRLEARIAELAAQEDLARVRPDLDGNQIMEILGIPAGPQVGEAWRFLKELRLERGPLDPDEATAELLSWWRSRGNG

Sequences:

>Translated_480_residues
MPDAAQDAELLTAAAVALNKHAPMLRELGSAFDAAGHQLYLVGGSVRDALLGRLSPDLDFTTDARPEQVQQILRRWADNL
WDTGIEFGTVGVGKGDRRLEITTFRADTYDQVSRNPQVRFGNRLEDDLVRRDFTANAMAVRITPDGPGEFLDPLGGLAAL
RQRVLDTPATPEESFGDDPLRMLRAARFVSQLGFTVAPRVREAIERMAPQLARISAERVAAELDKLVLGDDPVAGIDLLV
HSGMGEVVLPEIGGMQMAIDEHHQHKDVYQHSLTVLRQAIALEDDGPDLVLRWAALLHDIGKPATRRHEPNGGVSFHHHE
VVGAKMVRKRLRALKYSKQMIDDVSQLVYLHLRFHGYGDGKWTDSAVRRYVTDAGPLLPRLHKLVRADCTTRNKRRAARL
HASYDRLEARIAELAAQEDLARVRPDLDGNQIMEILGIPAGPQVGEAWRFLKELRLERGPLDPDEATAELLSWWRSRGNG
>Mature_479_residues
PDAAQDAELLTAAAVALNKHAPMLRELGSAFDAAGHQLYLVGGSVRDALLGRLSPDLDFTTDARPEQVQQILRRWADNLW
DTGIEFGTVGVGKGDRRLEITTFRADTYDQVSRNPQVRFGNRLEDDLVRRDFTANAMAVRITPDGPGEFLDPLGGLAALR
QRVLDTPATPEESFGDDPLRMLRAARFVSQLGFTVAPRVREAIERMAPQLARISAERVAAELDKLVLGDDPVAGIDLLVH
SGMGEVVLPEIGGMQMAIDEHHQHKDVYQHSLTVLRQAIALEDDGPDLVLRWAALLHDIGKPATRRHEPNGGVSFHHHEV
VGAKMVRKRLRALKYSKQMIDDVSQLVYLHLRFHGYGDGKWTDSAVRRYVTDAGPLLPRLHKLVRADCTTRNKRRAARLH
ASYDRLEARIAELAAQEDLARVRPDLDGNQIMEILGIPAGPQVGEAWRFLKELRLERGPLDPDEATAELLSWWRSRGNG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=233, Percent_Identity=30.0429184549356, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI87081691, Length=235, Percent_Identity=33.6170212765957, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789436, Length=335, Percent_Identity=30.7462686567164, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71995920, Length=212, Percent_Identity=30.188679245283, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=234, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI281360112, Length=214, Percent_Identity=29.9065420560748, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357337, Length=214, Percent_Identity=29.9065420560748, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 53431; Mature: 53300

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDAAQDAELLTAAAVALNKHAPMLRELGSAFDAAGHQLYLVGGSVRDALLGRLSPDLDF
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCC
TTDARPEQVQQILRRWADNLWDTGIEFGTVGVGKGDRRLEITTFRADTYDQVSRNPQVRF
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEEEECCCHHHHCCCCCCCC
GNRLEDDLVRRDFTANAMAVRITPDGPGEFLDPLGGLAALRQRVLDTPATPEESFGDDPL
CCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RMLRAARFVSQLGFTVAPRVREAIERMAPQLARISAERVAAELDKLVLGDDPVAGIDLLV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
HSGMGEVVLPEIGGMQMAIDEHHQHKDVYQHSLTVLRQAIALEDDGPDLVLRWAALLHDI
HCCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GKPATRRHEPNGGVSFHHHEVVGAKMVRKRLRALKYSKQMIDDVSQLVYLHLRFHGYGDG
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
KWTDSAVRRYVTDAGPLLPRLHKLVRADCTTRNKRRAARLHASYDRLEARIAELAAQEDL
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARVRPDLDGNQIMEILGIPAGPQVGEAWRFLKELRLERGPLDPDEATAELLSWWRSRGNG
HHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PDAAQDAELLTAAAVALNKHAPMLRELGSAFDAAGHQLYLVGGSVRDALLGRLSPDLDF
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCC
TTDARPEQVQQILRRWADNLWDTGIEFGTVGVGKGDRRLEITTFRADTYDQVSRNPQVRF
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEEEECCCHHHHCCCCCCCC
GNRLEDDLVRRDFTANAMAVRITPDGPGEFLDPLGGLAALRQRVLDTPATPEESFGDDPL
CCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RMLRAARFVSQLGFTVAPRVREAIERMAPQLARISAERVAAELDKLVLGDDPVAGIDLLV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
HSGMGEVVLPEIGGMQMAIDEHHQHKDVYQHSLTVLRQAIALEDDGPDLVLRWAALLHDI
HCCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GKPATRRHEPNGGVSFHHHEVVGAKMVRKRLRALKYSKQMIDDVSQLVYLHLRFHGYGDG
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
KWTDSAVRRYVTDAGPLLPRLHKLVRADCTTRNKRRAARLHASYDRLEARIAELAAQEDL
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARVRPDLDGNQIMEILGIPAGPQVGEAWRFLKELRLERGPLDPDEATAELLSWWRSRGNG
HHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA