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The map label for this gene is lipW

Identifier: 41409753

GI number: 41409753

Start: 4059631

End: 4060530

Strand: Reverse

Name: lipW

Synonym: MAP3655c

Alternate gene names: 41409753

Gene position: 4060530-4059631 (Counterclockwise)

Preceding gene: 41409759

Following gene: 41409749

Centisome position: 84.07

GC content: 71.89

Gene sequence:

>900_bases
GTGCCGAACAACGATGTTCATCCTGACCTGCAACGATTTGCCCGGTTCGCGCCGCGACGACTGGTCGGCCCGCGAACGCT
GCCGCTGATGCGGGTGGCGCTTGCCTGGCGCGGGCGGCTGGGCAAGCCGGTCCGCGACGTCGAGGTGATCACGCTCGGTT
CCGGGGTGGGGGTCCGCCTGTTCCGGCCCGCCGGCGCCGCGGAGACGACGCCCGCGCTGCTGTGGATCCACGGCGGCGGC
TACGTGCTGGGCACCGCCCAGCAGGACGACCAGCTGTGCAGCCGGTTCAGCCGACGGCTGGGCATCACGGTCGCATCGGT
GGATTACCGCCTGGCGCCCGAACATCGGTATCCGGCGCCGCTGGAGGACTGCTATACGGCGCTGACCTGGCTGGCCGGTC
TGCCGTCGGTGGACCGCTACGGCATCGCCATCGGCGGGGCCAGCGCCGGCGGCGGCCTGGCCGCGGCGCTGGCGCTGCTG
GCCCGCGACCGCGCCGAGGTGAGCCCCGCATTCCAGCTGCTGACCTATCCCATGCTCGACGACCGCAGCTCGGACACCGC
GCCCAGCCCGAACTATCGGCTGTGGGACAACCGCAGCAACAGGTTCGGCTGGGCGGCCTACCTCGGCGACGCCGACCCGC
ACGTCGCGGTGCCCGCCCGGCGGGACGACCTCAGCGGACTGCCGCCGGCCTGGATCGGGGTGGGCACCCATGACCTGTTT
CACGACGAGGACCTCGCCTACGCCGAACGGCTGCGGGCCGCCGGGGTGCCCTGCCAGGTGGAGACCATCCCCGGCGCCTT
CCACGGCTTCGACCTGATTTTGCCGAAAGTTCAAGTGTCGCAGCGGTTTTTCGACAGCCAGTGCAACAGCCTGCGGGCCG
CGCTGACGCCGGCCGGCTGA

Upstream 100 bases:

>100_bases
CGGTGCGGCCGGCGATCACCCGGCCGTCGGGCGGGCCGGTTCGCGGATCGCCTCGCCCTATAGCGGTGCCCCGCTGGTGG
CCGTCGGTGATACTGATCCG

Downstream 100 bases:

>100_bases
GGTCACATCGCGAAGTAGACGAGCTCACCGCCGATGATGGTGGCGGCCACCATGCCGGCGTCCAGTTCGGCCAGCGCGGT
GTGCGGCGGCTCGTTGAGCA

Product: LipW

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MPNNDVHPDLQRFARFAPRRLVGPRTLPLMRVALAWRGRLGKPVRDVEVITLGSGVGVRLFRPAGAAETTPALLWIHGGG
YVLGTAQQDDQLCSRFSRRLGITVASVDYRLAPEHRYPAPLEDCYTALTWLAGLPSVDRYGIAIGGASAGGGLAAALALL
ARDRAEVSPAFQLLTYPMLDDRSSDTAPSPNYRLWDNRSNRFGWAAYLGDADPHVAVPARRDDLSGLPPAWIGVGTHDLF
HDEDLAYAERLRAAGVPCQVETIPGAFHGFDLILPKVQVSQRFFDSQCNSLRAALTPAG

Sequences:

>Translated_299_residues
MPNNDVHPDLQRFARFAPRRLVGPRTLPLMRVALAWRGRLGKPVRDVEVITLGSGVGVRLFRPAGAAETTPALLWIHGGG
YVLGTAQQDDQLCSRFSRRLGITVASVDYRLAPEHRYPAPLEDCYTALTWLAGLPSVDRYGIAIGGASAGGGLAAALALL
ARDRAEVSPAFQLLTYPMLDDRSSDTAPSPNYRLWDNRSNRFGWAAYLGDADPHVAVPARRDDLSGLPPAWIGVGTHDLF
HDEDLAYAERLRAAGVPCQVETIPGAFHGFDLILPKVQVSQRFFDSQCNSLRAALTPAG
>Mature_298_residues
PNNDVHPDLQRFARFAPRRLVGPRTLPLMRVALAWRGRLGKPVRDVEVITLGSGVGVRLFRPAGAAETTPALLWIHGGGY
VLGTAQQDDQLCSRFSRRLGITVASVDYRLAPEHRYPAPLEDCYTALTWLAGLPSVDRYGIAIGGASAGGGLAAALALLA
RDRAEVSPAFQLLTYPMLDDRSSDTAPSPNYRLWDNRSNRFGWAAYLGDADPHVAVPARRDDLSGLPPAWIGVGTHDLFH
DEDLAYAERLRAAGVPCQVETIPGAFHGFDLILPKVQVSQRFFDSQCNSLRAALTPAG

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=297, Percent_Identity=28.2828282828283, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI206597554, Length=160, Percent_Identity=33.125, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI68299767, Length=139, Percent_Identity=36.6906474820144, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1786682, Length=243, Percent_Identity=30.0411522633745, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI72001146, Length=134, Percent_Identity=35.0746268656716, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17567059, Length=145, Percent_Identity=34.4827586206897, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71996133, Length=160, Percent_Identity=31.25, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17540028, Length=147, Percent_Identity=31.9727891156463, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 32460; Mature: 32329

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNDVHPDLQRFARFAPRRLVGPRTLPLMRVALAWRGRLGKPVRDVEVITLGSGVGVRL
CCCCCCCHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEE
FRPAGAAETTPALLWIHGGGYVLGTAQQDDQLCSRFSRRLGITVASVDYRLAPEHRYPAP
ECCCCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHCCEEEEECEEECCCCCCCCC
LEDCYTALTWLAGLPSVDRYGIAIGGASAGGGLAAALALLARDRAEVSPAFQLLTYPMLD
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
DRSSDTAPSPNYRLWDNRSNRFGWAAYLGDADPHVAVPARRDDLSGLPPAWIGVGTHDLF
CCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCEECCCCCCCCCCCCCCEECCCCHHHC
HDEDLAYAERLRAAGVPCQVETIPGAFHGFDLILPKVQVSQRFFDSQCNSLRAALTPAG
CCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PNNDVHPDLQRFARFAPRRLVGPRTLPLMRVALAWRGRLGKPVRDVEVITLGSGVGVRL
CCCCCCHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEE
FRPAGAAETTPALLWIHGGGYVLGTAQQDDQLCSRFSRRLGITVASVDYRLAPEHRYPAP
ECCCCCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHCCEEEEECEEECCCCCCCCC
LEDCYTALTWLAGLPSVDRYGIAIGGASAGGGLAAALALLARDRAEVSPAFQLLTYPMLD
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
DRSSDTAPSPNYRLWDNRSNRFGWAAYLGDADPHVAVPARRDDLSGLPPAWIGVGTHDLF
CCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCEECCCCCCCCCCCCCCEECCCCHHHC
HDEDLAYAERLRAAGVPCQVETIPGAFHGFDLILPKVQVSQRFFDSQCNSLRAALTPAG
CCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]