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The map label for this gene is mcbR [H]

Identifier: 41409697

GI number: 41409697

Start: 3995448

End: 3996263

Strand: Reverse

Name: mcbR [H]

Synonym: MAP3599c

Alternate gene names: 41409697

Gene position: 3996263-3995448 (Counterclockwise)

Preceding gene: 41409713

Following gene: 41409694

Centisome position: 82.74

GC content: 70.59

Gene sequence:

>816_bases
GTGTTGTGTGGGTCACATTATGGCGTTGACGGTCTACTGACAAGCCCCCGTCGACACCGGTCAAAACGTAAACCCCGTCC
CGCTAAGCCACTGAAAGCCCTGGCAAGCCCCGTGAACGCACCGATGTCCATGCAGCCGAGGAGCCGGCGGCGGCCGCTGC
GCCGGGCCCAGTTGTCCGACGAGGTCGCGGGGCACCTGCGGGCCGCGATCATGTCCGGCGCGCTGCGGCCGGGCACCTTC
ATCCGGCTCGACGAGACCGCGGCCGAGCTCGGCGTCAGCGTCACGCCGGTGCGCGAGGCCCTGCTGAAGCTGCGCGGCGA
GGGCATGGTGCAGCTCGAACCGCACCGCGGTCACGTGGTGCTGCCGCTCACCCGCCAAGACATCGAGGACATCTTCTGGC
TGCAGGCGACCATCGCCAAGGAGCTGGCCGCCGCGGCCACCGACCACATCACCGACACCGAGATCGACGAGCTGGATCGC
ATCAACGACGCGCTCGCCGCGGCCGTCGGGTCCGGCGACGCCGAGACCATCGCCGGCCTCGAGTTCGGCTTCCACCGGGT
CTTCAACCAGGCCAGCGGCCGGATCAAGCTGGCCTGGTTCCTGCTCAACGCCGCCCGCTACATGCCGGTGCTGGTGTACG
CCGCCGACCCGCAGTGGGGGCGGGCCGCCGTCGACAACCACCGGCAACTGATCGCCGCGCTGCGCCGCCGCGACACCGCC
GCGGTGATCGAGCACACGGTCTGGCAGTTCACCGACGCGGCGGCGCGGCTGACCGAGATGCGCGACCGCACCGGCACGCC
GAGCAACCGGGGTTAG

Upstream 100 bases:

>100_bases
TCGTTCGCCTGAGTCGGATGGTGTGCCAATTGGGCGGTCAAACTCAACACTTCCCTTCCCTTCACTCGCGCACCGCTTGC
GCCAGGTTTGATAAAACTTA

Downstream 100 bases:

>100_bases
TTCGCGGCCAGCTGCTCGGCGCGCTGCTTGAGGTTGTCGGCCAGGTGGTCGAGCACGTTGTTCAGCATCGACTTCACCAT
CGGCGCGGGAACCGGCATCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MLCGSHYGVDGLLTSPRRHRSKRKPRPAKPLKALASPVNAPMSMQPRSRRRPLRRAQLSDEVAGHLRAAIMSGALRPGTF
IRLDETAAELGVSVTPVREALLKLRGEGMVQLEPHRGHVVLPLTRQDIEDIFWLQATIAKELAAAATDHITDTEIDELDR
INDALAAAVGSGDAETIAGLEFGFHRVFNQASGRIKLAWFLLNAARYMPVLVYAADPQWGRAAVDNHRQLIAALRRRDTA
AVIEHTVWQFTDAAARLTEMRDRTGTPSNRG

Sequences:

>Translated_271_residues
MLCGSHYGVDGLLTSPRRHRSKRKPRPAKPLKALASPVNAPMSMQPRSRRRPLRRAQLSDEVAGHLRAAIMSGALRPGTF
IRLDETAAELGVSVTPVREALLKLRGEGMVQLEPHRGHVVLPLTRQDIEDIFWLQATIAKELAAAATDHITDTEIDELDR
INDALAAAVGSGDAETIAGLEFGFHRVFNQASGRIKLAWFLLNAARYMPVLVYAADPQWGRAAVDNHRQLIAALRRRDTA
AVIEHTVWQFTDAAARLTEMRDRTGTPSNRG
>Mature_271_residues
MLCGSHYGVDGLLTSPRRHRSKRKPRPAKPLKALASPVNAPMSMQPRSRRRPLRRAQLSDEVAGHLRAAIMSGALRPGTF
IRLDETAAELGVSVTPVREALLKLRGEGMVQLEPHRGHVVLPLTRQDIEDIFWLQATIAKELAAAATDHITDTEIDELDR
INDALAAAVGSGDAETIAGLEFGFHRVFNQASGRIKLAWFLLNAARYMPVLVYAADPQWGRAAVDNHRQLIAALRRRDTA
AVIEHTVWQFTDAAARLTEMRDRTGTPSNRG

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=190, Percent_Identity=26.8421052631579, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 29909; Mature: 29909

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCGSHYGVDGLLTSPRRHRSKRKPRPAKPLKALASPVNAPMSMQPRSRRRPLRRAQLSD
CCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHH
EVAGHLRAAIMSGALRPGTFIRLDETAAELGVSVTPVREALLKLRGEGMVQLEPHRGHVV
HHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCEEE
LPLTRQDIEDIFWLQATIAKELAAAATDHITDTEIDELDRINDALAAAVGSGDAETIAGL
EEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
EFGFHRVFNQASGRIKLAWFLLNAARYMPVLVYAADPQWGRAAVDNHRQLIAALRRRDTA
HHHHHHHHHHCCCCEEEHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHH
AVIEHTVWQFTDAAARLTEMRDRTGTPSNRG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLCGSHYGVDGLLTSPRRHRSKRKPRPAKPLKALASPVNAPMSMQPRSRRRPLRRAQLSD
CCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHH
EVAGHLRAAIMSGALRPGTFIRLDETAAELGVSVTPVREALLKLRGEGMVQLEPHRGHVV
HHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCEEE
LPLTRQDIEDIFWLQATIAKELAAAATDHITDTEIDELDRINDALAAAVGSGDAETIAGL
EEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
EFGFHRVFNQASGRIKLAWFLLNAARYMPVLVYAADPQWGRAAVDNHRQLIAALRRRDTA
HHHHHHHHHHCCCCEEEHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHH
AVIEHTVWQFTDAAARLTEMRDRTGTPSNRG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]