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The map label for this gene is hdhA [C]

Identifier: 41409320

GI number: 41409320

Start: 3574745

End: 3575551

Strand: Reverse

Name: hdhA [C]

Synonym: MAP3222c

Alternate gene names: 41409320

Gene position: 3575551-3574745 (Counterclockwise)

Preceding gene: 41409321

Following gene: 41409318

Centisome position: 74.03

GC content: 70.88

Gene sequence:

>807_bases
GTGGCGGTAGCCGGGCGGACCGAGCAAGTCTGGGACGACCGGTTGCCGGGCACCATCGGGTCCACGGTCGCCGACATCGA
GGCCGCCGGTGGGCGCGCGGTGCCGGTGCGGGCCGATCTGACCGACCGCGACGACGTCGCGCGGCTGGTGGATTCGGCTC
GAGAGGCGTTGGGCCCCATCACGATTCTGGTCAATAATGCGGCCTTCACCGCGCCCGGCAGACCGCCGGCGCCCGGTGGT
GAGGCGCGCGCCAAACCGGCGGCCGGCGGCGCCAAACCAGCGTCGGGCGGCGCCAAACCGGCGTCGGGCGGCGCCAAACC
GGGCTGGCCGGGCTTCGTCAGCACGCCGCTGCACGCCTACCGGCGGCATTTCGACATCGCCGTCTTCGCCGCCTACGAAC
TGATGCAGAGGGTGTGCCCGGACATGATCGGGGCGGGCGGCGGAGCGATCATCAACATCACCTCGGTCGCCTCCCGGCTG
CCGGGTGACGGCCCGTACGCCGATCGCAGCGGCGGGGTGCTGCCCGGGTACGGCGGGTCCAAGGCCGCGCTCGAACACCT
GACCCAGTGTGTGGCATACGATCTCGCCGACCATCGCATCGCGGTCAATGCGCTGTCGCCGTCGAAACCGATCCTCACGC
CGGGGCTTTCCTACTACGCCCGCGATTTCGACGACACCGCGTCGGCGGACGAATTCGCCCGGGCCGCAGTGGAATTGGCG
CTGGTAGATCCCGGCAGGGTCACCGGACGCACCATCGGTCACCTGCAGGTGCTGGACGGCAGCTTCCGCCCCTTCGGCTT
GGACTAG

Upstream 100 bases:

>100_bases
GGTGACGGGGCGGCTGACCGGCCGCGCCGCGATCGTCACCGGCGCCAGCCGCGGCCTGGGCCGGGCCATCGCGCTGGCCC
TGGCGAGCGAGGGCGCCGCG

Downstream 100 bases:

>100_bases
GTTCTGGCGGCTTTAGGTTCTTCTGGCCCGCATCACCGCGGCGGGGGAGGCGTTGCACCGTCCCCCACCGCGGCTCACGG
GTTTAGCGACCCGGAGGCCC

Product: hypothetical protein

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: Short-Chain Type Dehydrogenase/Reductase

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MAVAGRTEQVWDDRLPGTIGSTVADIEAAGGRAVPVRADLTDRDDVARLVDSAREALGPITILVNNAAFTAPGRPPAPGG
EARAKPAAGGAKPASGGAKPASGGAKPGWPGFVSTPLHAYRRHFDIAVFAAYELMQRVCPDMIGAGGGAIINITSVASRL
PGDGPYADRSGGVLPGYGGSKAALEHLTQCVAYDLADHRIAVNALSPSKPILTPGLSYYARDFDDTASADEFARAAVELA
LVDPGRVTGRTIGHLQVLDGSFRPFGLD

Sequences:

>Translated_268_residues
MAVAGRTEQVWDDRLPGTIGSTVADIEAAGGRAVPVRADLTDRDDVARLVDSAREALGPITILVNNAAFTAPGRPPAPGG
EARAKPAAGGAKPASGGAKPASGGAKPGWPGFVSTPLHAYRRHFDIAVFAAYELMQRVCPDMIGAGGGAIINITSVASRL
PGDGPYADRSGGVLPGYGGSKAALEHLTQCVAYDLADHRIAVNALSPSKPILTPGLSYYARDFDDTASADEFARAAVELA
LVDPGRVTGRTIGHLQVLDGSFRPFGLD
>Mature_267_residues
AVAGRTEQVWDDRLPGTIGSTVADIEAAGGRAVPVRADLTDRDDVARLVDSAREALGPITILVNNAAFTAPGRPPAPGGE
ARAKPAAGGAKPASGGAKPASGGAKPGWPGFVSTPLHAYRRHFDIAVFAAYELMQRVCPDMIGAGGGAIINITSVASRLP
GDGPYADRSGGVLPGYGGSKAALEHLTQCVAYDLADHRIAVNALSPSKPILTPGLSYYARDFDDTASADEFARAAVELAL
VDPGRVTGRTIGHLQVLDGSFRPFGLD

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787905, Length=195, Percent_Identity=31.7948717948718, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 27549; Mature: 27418

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAGRTEQVWDDRLPGTIGSTVADIEAAGGRAVPVRADLTDRDDVARLVDSAREALGPI
CCCCCCCHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCE
TILVNNAAFTAPGRPPAPGGEARAKPAAGGAKPASGGAKPASGGAKPGWPGFVSTPLHAY
EEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RRHFDIAVFAAYELMQRVCPDMIGAGGGAIINITSVASRLPGDGPYADRSGGVLPGYGGS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCH
KAALEHLTQCVAYDLADHRIAVNALSPSKPILTPGLSYYARDFDDTASADEFARAAVELA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHEEE
LVDPGRVTGRTIGHLQVLDGSFRPFGLD
EECCCCCCCCEECEEEEECCCCCCCCCC
>Mature Secondary Structure 
AVAGRTEQVWDDRLPGTIGSTVADIEAAGGRAVPVRADLTDRDDVARLVDSAREALGPI
CCCCCCHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCE
TILVNNAAFTAPGRPPAPGGEARAKPAAGGAKPASGGAKPASGGAKPGWPGFVSTPLHAY
EEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RRHFDIAVFAAYELMQRVCPDMIGAGGGAIINITSVASRLPGDGPYADRSGGVLPGYGGS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCH
KAALEHLTQCVAYDLADHRIAVNALSPSKPILTPGLSYYARDFDDTASADEFARAAVELA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHEEE
LVDPGRVTGRTIGHLQVLDGSFRPFGLD
EECCCCCCCCEECEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA