Click here to switch to the map view.

The map label for this gene is nuoM

Identifier: 41409311

GI number: 41409311

Start: 3563642

End: 3565210

Strand: Direct

Name: nuoM

Synonym: MAP3213

Alternate gene names: 41409311

Gene position: 3563642-3565210 (Clockwise)

Preceding gene: 41409310

Following gene: 41409312

Centisome position: 73.78

GC content: 67.24

Gene sequence:

>1569_bases
GTGAATCACCTGCCCTGGCTGAGCATCCTGTGGCTGGTGCCGCTGGCCGGCTCGGTACTGATCATCCTGATGCCGCCCGG
GCTGCGGCAGCTCGCCAAGTGGACCGGCCTGGTGTTCGGGGTGCTGACCCTGGCGGTGGCGATCGTGGTCACCGCCGGCT
TCAAAACCGGCGGCGCCCCCTATCAGTTCGTCGAAAAACACCAGTGGATCCCGGCTTTCGGCGCCGGCTACACGCTGGGC
GTGGACGGCATCGCGGTGGTGCTGGTGCTGCTGACCGCGGTGCTGATCCCGGTGCTGCAGGTGGCCGGCTGGAACGACGG
CGGCGATCGGCAGCGCGGCGTGCACGCCTACGTCGCCCTGACGCTGGCCATCGAGTCGATGGTGCTGGTCTCGGTGATCG
CCCTGGACGTGTTGCTGTTCTACGTGTTCTTCGAGGCGATGCTCATCCCGATGTACTTCCTGATCGGCGGCTTCGGCGCC
GGATCGGGCAGGTCGCGCGCGGCGGTGAAGTTCTTGCTGTACAACCTGTTCGGCGGGCTCATCATGCTGGCCGCGGTGAT
CGGGCTGTACGTGGTGACTTCCCAGCACGGGGCCGGCACCTTCGACTTCCGCGACATCGTGGCCGGGGTGGCCTCCGGCC
GGTACGGCGCCGACCCGGCGGTGTCCAAGGCGCTGTTCCTCGGCTTCATGTTCGCGTTCGCGATCAAGGCGCCGCTGTGG
CCTTTCCACCGCTGGCTGCCCGACGCCGCGGTCGAGGCGACGCCGGCGACCGCGGTGCTGATGATGGCGGTGATGGACAA
GGTCGGCACGTTCGGCATGCTGCGCTACTGCCTGCAGCTGTTCCCGGACGCCGCAACGTATTTCCGCCCGCTGATCGTGG
TGCTGGCGGTCATCGGGGTGATCTACGGGGCGATCGTCGCGATCGGTCAGGCCGATGTCATGCGGCTGATCGCCTACACC
TCGATCTCGCACTTCGGGTTCATCATCCTGGGCATCTTCGTGATGACCAGCCAGGGCCAGAGCGGCTCGACGCTCTACAT
GCTCAACCACGGCCTGTCCACCGCGGCGGTGTTCCTGATCGCCGGGTTCCTGATATCGCGGCACGGCGGCAGTCGGGCCA
TCGTCGACTACGGCGGCGTGCAGAAGGTGGCGCCGGTGCTGGCCGGCACCTTCCTGGTTTCGGCCATGGCCACCCTCTCG
CTGCCCGGTCTGGCGCCGTTCGTCAGCGAATTCCTGGTCCTGCTGGGCACTTTCAACCGATATTGGCTGGCGGCCGCGTT
CGGGGTCACCGCGCTGGTGCTCTCCGCGGTGTACATGTTGTGGCTCTACCAGCGCATGATGACCGGACCGGTGGCCGGCG
GCAACGAGAAGATCGGCGATCTGCGGGCCCGGGAGTTGGTCGTGGTGGCGCCGCTGATCGCGCTGCTGCTGGTGCTCGGC
ATCTATCCCAAGCCGGTGCTCGACATCATCAACCCCGCCGTCGAGAACACCATGACCACCATCGGTCAGCACGACCCCGC
TCCGAGCGTGCCGATGGGCACGCCGCGGACAGCCGAAGGACGGCGATGA

Upstream 100 bases:

>100_bases
GCTTGCGGGGCCTGCAAACCGGCTTCGCCCGCAACTATGCCCTGTCGATGCTGACCGGCGCCGTGCTGGTGATCGCGCTG
ATCCTGGCGGTGCGGCTGTG

Downstream 100 bases:

>100_bases
TGACCCTGCCCACCCCGAGCATCCAGTACTTCCTGCTGTGCCCGACGCTGATCGTGTTCGGCGTCGCCGTGGCCGGTGTT
CTGGCGGAAGCGTTCCTGCC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MNHLPWLSILWLVPLAGSVLIILMPPGLRQLAKWTGLVFGVLTLAVAIVVTAGFKTGGAPYQFVEKHQWIPAFGAGYTLG
VDGIAVVLVLLTAVLIPVLQVAGWNDGGDRQRGVHAYVALTLAIESMVLVSVIALDVLLFYVFFEAMLIPMYFLIGGFGA
GSGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTSQHGAGTFDFRDIVAGVASGRYGADPAVSKALFLGFMFAFAIKAPLW
PFHRWLPDAAVEATPATAVLMMAVMDKVGTFGMLRYCLQLFPDAATYFRPLIVVLAVIGVIYGAIVAIGQADVMRLIAYT
SISHFGFIILGIFVMTSQGQSGSTLYMLNHGLSTAAVFLIAGFLISRHGGSRAIVDYGGVQKVAPVLAGTFLVSAMATLS
LPGLAPFVSEFLVLLGTFNRYWLAAAFGVTALVLSAVYMLWLYQRMMTGPVAGGNEKIGDLRARELVVVAPLIALLLVLG
IYPKPVLDIINPAVENTMTTIGQHDPAPSVPMGTPRTAEGRR

Sequences:

>Translated_522_residues
MNHLPWLSILWLVPLAGSVLIILMPPGLRQLAKWTGLVFGVLTLAVAIVVTAGFKTGGAPYQFVEKHQWIPAFGAGYTLG
VDGIAVVLVLLTAVLIPVLQVAGWNDGGDRQRGVHAYVALTLAIESMVLVSVIALDVLLFYVFFEAMLIPMYFLIGGFGA
GSGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTSQHGAGTFDFRDIVAGVASGRYGADPAVSKALFLGFMFAFAIKAPLW
PFHRWLPDAAVEATPATAVLMMAVMDKVGTFGMLRYCLQLFPDAATYFRPLIVVLAVIGVIYGAIVAIGQADVMRLIAYT
SISHFGFIILGIFVMTSQGQSGSTLYMLNHGLSTAAVFLIAGFLISRHGGSRAIVDYGGVQKVAPVLAGTFLVSAMATLS
LPGLAPFVSEFLVLLGTFNRYWLAAAFGVTALVLSAVYMLWLYQRMMTGPVAGGNEKIGDLRARELVVVAPLIALLLVLG
IYPKPVLDIINPAVENTMTTIGQHDPAPSVPMGTPRTAEGRR
>Mature_522_residues
MNHLPWLSILWLVPLAGSVLIILMPPGLRQLAKWTGLVFGVLTLAVAIVVTAGFKTGGAPYQFVEKHQWIPAFGAGYTLG
VDGIAVVLVLLTAVLIPVLQVAGWNDGGDRQRGVHAYVALTLAIESMVLVSVIALDVLLFYVFFEAMLIPMYFLIGGFGA
GSGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTSQHGAGTFDFRDIVAGVASGRYGADPAVSKALFLGFMFAFAIKAPLW
PFHRWLPDAAVEATPATAVLMMAVMDKVGTFGMLRYCLQLFPDAATYFRPLIVVLAVIGVIYGAIVAIGQADVMRLIAYT
SISHFGFIILGIFVMTSQGQSGSTLYMLNHGLSTAAVFLIAGFLISRHGGSRAIVDYGGVQKVAPVLAGTFLVSAMATLS
LPGLAPFVSEFLVLLGTFNRYWLAAAFGVTALVLSAVYMLWLYQRMMTGPVAGGNEKIGDLRARELVVVAPLIALLLVLG
IYPKPVLDIINPAVENTMTTIGQHDPAPSVPMGTPRTAEGRR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=376, Percent_Identity=28.1914893617021, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI251831117, Length=340, Percent_Identity=21.7647058823529, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1788613, Length=507, Percent_Identity=34.3195266272189, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1788827, Length=311, Percent_Identity=27.9742765273312, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1788831, Length=314, Percent_Identity=26.4331210191083, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788614, Length=413, Percent_Identity=26.8765133171913, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693160, Length=408, Percent_Identity=24.2647058823529, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788829, Length=436, Percent_Identity=25.2293577981651, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367154, Length=295, Percent_Identity=28.4745762711864, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55657; Mature: 55657

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00673 V8_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLPWLSILWLVPLAGSVLIILMPPGLRQLAKWTGLVFGVLTLAVAIVVTAGFKTGGAP
CCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YQFVEKHQWIPAFGAGYTLGVDGIAVVLVLLTAVLIPVLQVAGWNDGGDRQRGVHAYVAL
HHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TLAIESMVLVSVIALDVLLFYVFFEAMLIPMYFLIGGFGAGSGRSRAAVKFLLYNLFGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
IMLAAVIGLYVVTSQHGAGTFDFRDIVAGVASGRYGADPAVSKALFLGFMFAFAIKAPLW
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PFHRWLPDAAVEATPATAVLMMAVMDKVGTFGMLRYCLQLFPDAATYFRPLIVVLAVIGV
HHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IYGAIVAIGQADVMRLIAYTSISHFGFIILGIFVMTSQGQSGSTLYMLNHGLSTAAVFLI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHH
AGFLISRHGGSRAIVDYGGVQKVAPVLAGTFLVSAMATLSLPGLAPFVSEFLVLLGTFNR
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YWLAAAFGVTALVLSAVYMLWLYQRMMTGPVAGGNEKIGDLRARELVVVAPLIALLLVLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
IYPKPVLDIINPAVENTMTTIGQHDPAPSVPMGTPRTAEGRR
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]