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The map label for this gene is rlmN [H]

Identifier: 41409041

GI number: 41409041

Start: 3278290

End: 3279384

Strand: Reverse

Name: rlmN [H]

Synonym: MAP2943c

Alternate gene names: 41409041

Gene position: 3279384-3278290 (Counterclockwise)

Preceding gene: 41409042

Following gene: 41409040

Centisome position: 67.9

GC content: 72.15

Gene sequence:

>1095_bases
ATGGTTCAACAACTGGTGTTCTCCGAGCCGCGCCCCGGTAAGCCGCCGCGGCACCTGGCCGATCTCGACGCCGACGGCCG
GGCCTCGGCCGTCGCCGAGCTGGGCTTGCCGGCGTTTCGGGCCAAGCAGCTGGCGCATCAGTACTACGGCCGGCTGATCG
CCGATCCGCGCCAGATGACCGATCTGCCGGCCGGGCTGCGCGACGCGATCGCCGACACCATGTTCCCGATCCTGCTCACC
GCGGCCTCCGAGGTGACCTGCGATGCCGGCCAGACCCGAAAGACGTTGTGGCGGGCCCTGGATGGGGTCACCGTGGAGTC
GGTGCTGATGCGCTACCCGCACCGCAACACGGTGTGCATCTCGTCGCAGGCCGGCTGCGGCATGGCCTGCCCGTTCTGCG
CGACCGGCCAGGGCGGGCTGAGCCGCAACCTGTCGACGGCCGAGATCCTCGAGCAGGTGCGCGCCGGCGCGGCGGCGCTG
CGCGACGACTTCGGTGACCGCCTGTCCAACGTGGTGTTCATGGGCATGGGGGAGCCGCTGGCCAACTACGCGCGGGTGGT
GGCCGCGGTGCGGCGCATCGTCGCCGCGCCGCCGCAGGGTTTCGGCATCTCGGCCCGTTCGGTGACGGTGTCGACGGTGG
GGCTGGCGCCGGCGATCCGCAAACTCGCCGACGAACGGCTCGGGGTGACGCTGGCGTTGTCGCTGCACGCCCCCGACGAC
GAGCTGCGCGACACGCTGGTGCCGGTCAACAACCGGTGGAAGATCGCCGAGGCGCTCGACGCCGCCCGGTATTACGCCGA
CGTCACCGGCCGTCGGGTGTCGGTGGAGTACGCGTTGATCCGCGACGTCAACGATCAGCCGTGGCGCGCCGATCTGTTGG
GGCGGCGGCTGCATCGCGCGCTCGGGCCGCTGGTGCACGTGAACCTGATACCGCTCAACCCGACGCCGGGCAGCCAGTGG
GACGCCAGTCCCAAGCCGGTCGAGCGGGAGTTCGTCCGGCGGGTGCGCGCCGCCGGGGTGTCCTGCACGGTGCGCGACAC
CCGCGGCCGCGAGATCAGCGCCGCCTGCGGACAGCTGGCCGCCGAAGGCGGGTAG

Upstream 100 bases:

>100_bases
GACCGGCTGGACGGCGTGCTGCCGTCGGCGGTCGCCGCCTGGACGGTGCTGACGCTCGTTCCGTAGGCCGGCCCCGGCGA
TGACCGATACTGGACGGGTC

Downstream 100 bases:

>100_bases
CTGGAGCGCAGGCGATCGGGCCTGGGCCTGCGGACAGCTCGCCGCCGAAGGCGGGTAGCTGGCCTTCGAGAAGGGATAGC
AGGCGGGGCCGGGCGGTGAA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MVQQLVFSEPRPGKPPRHLADLDADGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMTDLPAGLRDAIADTMFPILLT
AASEVTCDAGQTRKTLWRALDGVTVESVLMRYPHRNTVCISSQAGCGMACPFCATGQGGLSRNLSTAEILEQVRAGAAAL
RDDFGDRLSNVVFMGMGEPLANYARVVAAVRRIVAAPPQGFGISARSVTVSTVGLAPAIRKLADERLGVTLALSLHAPDD
ELRDTLVPVNNRWKIAEALDAARYYADVTGRRVSVEYALIRDVNDQPWRADLLGRRLHRALGPLVHVNLIPLNPTPGSQW
DASPKPVEREFVRRVRAAGVSCTVRDTRGREISAACGQLAAEGG

Sequences:

>Translated_364_residues
MVQQLVFSEPRPGKPPRHLADLDADGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMTDLPAGLRDAIADTMFPILLT
AASEVTCDAGQTRKTLWRALDGVTVESVLMRYPHRNTVCISSQAGCGMACPFCATGQGGLSRNLSTAEILEQVRAGAAAL
RDDFGDRLSNVVFMGMGEPLANYARVVAAVRRIVAAPPQGFGISARSVTVSTVGLAPAIRKLADERLGVTLALSLHAPDD
ELRDTLVPVNNRWKIAEALDAARYYADVTGRRVSVEYALIRDVNDQPWRADLLGRRLHRALGPLVHVNLIPLNPTPGSQW
DASPKPVEREFVRRVRAAGVSCTVRDTRGREISAACGQLAAEGG
>Mature_364_residues
MVQQLVFSEPRPGKPPRHLADLDADGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMTDLPAGLRDAIADTMFPILLT
AASEVTCDAGQTRKTLWRALDGVTVESVLMRYPHRNTVCISSQAGCGMACPFCATGQGGLSRNLSTAEILEQVRAGAAAL
RDDFGDRLSNVVFMGMGEPLANYARVVAAVRRIVAAPPQGFGISARSVTVSTVGLAPAIRKLADERLGVTLALSLHAPDD
ELRDTLVPVNNRWKIAEALDAARYYADVTGRRVSVEYALIRDVNDQPWRADLLGRRLHRALGPLVHVNLIPLNPTPGSQW
DASPKPVEREFVRRVRAAGVSCTVRDTRGREISAACGQLAAEGG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=374, Percent_Identity=37.9679144385027, Blast_Score=201, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 39257; Mature: 39257

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQQLVFSEPRPGKPPRHLADLDADGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMT
CCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
DLPAGLRDAIADTMFPILLTAASEVTCDAGQTRKTLWRALDGVTVESVLMRYPHRNTVCI
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCEEEE
SSQAGCGMACPFCATGQGGLSRNLSTAEILEQVRAGAAALRDDFGDRLSNVVFMGMGEPL
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHH
ANYARVVAAVRRIVAAPPQGFGISARSVTVSTVGLAPAIRKLADERLGVTLALSLHAPDD
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCEEEEEEEECCCH
ELRDTLVPVNNRWKIAEALDAARYYADVTGRRVSVEYALIRDVNDQPWRADLLGRRLHRA
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHH
LGPLVHVNLIPLNPTPGSQWDASPKPVEREFVRRVRAAGVSCTVRDTRGREISAACGQLA
HCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
AEGG
CCCC
>Mature Secondary Structure
MVQQLVFSEPRPGKPPRHLADLDADGRASAVAELGLPAFRAKQLAHQYYGRLIADPRQMT
CCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
DLPAGLRDAIADTMFPILLTAASEVTCDAGQTRKTLWRALDGVTVESVLMRYPHRNTVCI
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCEEEE
SSQAGCGMACPFCATGQGGLSRNLSTAEILEQVRAGAAALRDDFGDRLSNVVFMGMGEPL
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHH
ANYARVVAAVRRIVAAPPQGFGISARSVTVSTVGLAPAIRKLADERLGVTLALSLHAPDD
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCEEEEEEEECCCH
ELRDTLVPVNNRWKIAEALDAARYYADVTGRRVSVEYALIRDVNDQPWRADLLGRRLHRA
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHH
LGPLVHVNLIPLNPTPGSQWDASPKPVEREFVRRVRAAGVSCTVRDTRGREISAACGQLA
HCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
AEGG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA