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The map label for this gene is bphE [H]

Identifier: 41408833

GI number: 41408833

Start: 3068621

End: 3069175

Strand: Direct

Name: bphE [H]

Synonym: MAP2735

Alternate gene names: 41408833

Gene position: 3068621-3069175 (Clockwise)

Preceding gene: 41408832

Following gene: 41408834

Centisome position: 63.54

GC content: 67.57

Gene sequence:

>555_bases
GTGGGCCGACTATCTGCAGGAAGGCGACGCATGCGAAAACCGTTGACCTGCCTGCCCTTTGACGATGCTCGGCACCTGCA
GGCGCACCAGTTCCTGGTGGACGAGGCCTACCTGCTCGACGCCCAGCACTACCAGGCGTGGCTGGACACGCTGACCGACG
ACGTCCGCTACGTCATGCCGGTGCGGGTGACCACCGCGCGCGGCGCGGGGTTCGACACCAGCCCGGGGATGGCGCACTTC
GACGAGGACAAGTACTCGCTGAGCCAGCGGGTCGCGCGGTTCGCCACCGAGCACGCCTGGACCGAGGATCCGCCGTCGCG
GCTGCGGCATTTCGTCACCAACGTGCGCACCTTCGTTGAGGACGACCGGCACCTGCTGGTCGAGTCCGCCGAGCTGCTGT
TCCGCAGCCGCGGCGACGTCAACGAGAGCGCCCTGGTGTCCTGCGGCCGCGAGGACGTGCTGCGCTGGTCTGAGGATCGC
TGGAAGTTGGCCCGCCGCAGCATCTTCGTCGACGAGTCGGTGCTGCGGATGCAGAACCTGGCGGTGTTTTTGTGA

Upstream 100 bases:

>100_bases
GCCGCCCGGTCGTCGAGGCGCTGGCGCCCAGCGCTTTCCACGGCCCCGGACGCGCGCAGGTCGGCTACAACGAGCACAAC
CAGCGGGCGCTGCTGGCCAT

Downstream 100 bases:

>100_bases
ATCCCGCCGGCGAACCCATCATCCTCGGCAATGAATTCACCGAGGTGCGGGTGCAGCGGGTCGAGACTCGCAACGGGTCA
CGGCTGCTGATCAGCGCCCC

Product: 3-phenylpropionate dioxygenase subunit beta

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MGRLSAGRRRMRKPLTCLPFDDARHLQAHQFLVDEAYLLDAQHYQAWLDTLTDDVRYVMPVRVTTARGAGFDTSPGMAHF
DEDKYSLSQRVARFATEHAWTEDPPSRLRHFVTNVRTFVEDDRHLLVESAELLFRSRGDVNESALVSCGREDVLRWSEDR
WKLARRSIFVDESVLRMQNLAVFL

Sequences:

>Translated_184_residues
MGRLSAGRRRMRKPLTCLPFDDARHLQAHQFLVDEAYLLDAQHYQAWLDTLTDDVRYVMPVRVTTARGAGFDTSPGMAHF
DEDKYSLSQRVARFATEHAWTEDPPSRLRHFVTNVRTFVEDDRHLLVESAELLFRSRGDVNESALVSCGREDVLRWSEDR
WKLARRSIFVDESVLRMQNLAVFL
>Mature_183_residues
GRLSAGRRRMRKPLTCLPFDDARHLQAHQFLVDEAYLLDAQHYQAWLDTLTDDVRYVMPVRVTTARGAGFDTSPGMAHFD
EDKYSLSQRVARFATEHAWTEDPPSRLRHFVTNVRTFVEDDRHLLVESAELLFRSRGDVNESALVSCGREDVLRWSEDRW
KLARRSIFVDESVLRMQNLAVFL

Specific function: The beta subunit may be responsible for the substrate specificity of the enzyme [H]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788889, Length=164, Percent_Identity=34.1463414634146, Blast_Score=88, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 21425; Mature: 21294

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLSAGRRRMRKPLTCLPFDDARHLQAHQFLVDEAYLLDAQHYQAWLDTLTDDVRYVMP
CCCCCHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VRVTTARGAGFDTSPGMAHFDEDKYSLSQRVARFATEHAWTEDPPSRLRHFVTNVRTFVE
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
DDRHLLVESAELLFRSRGDVNESALVSCGREDVLRWSEDRWKLARRSIFVDESVLRMQNL
CHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AVFL
HHCC
>Mature Secondary Structure 
GRLSAGRRRMRKPLTCLPFDDARHLQAHQFLVDEAYLLDAQHYQAWLDTLTDDVRYVMP
CCCCHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VRVTTARGAGFDTSPGMAHFDEDKYSLSQRVARFATEHAWTEDPPSRLRHFVTNVRTFVE
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
DDRHLLVESAELLFRSRGDVNESALVSCGREDVLRWSEDRWKLARRSIFVDESVLRMQNL
CHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AVFL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8890734 [H]