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The map label for this gene is bphA [H]

Identifier: 41408832

GI number: 41408832

Start: 3067326

End: 3068666

Strand: Direct

Name: bphA [H]

Synonym: MAP2734

Alternate gene names: 41408832

Gene position: 3067326-3068666 (Clockwise)

Preceding gene: 41408826

Following gene: 41408833

Centisome position: 63.51

GC content: 66.82

Gene sequence:

>1341_bases
TTGCCCGGGGTGCTCGAGAACGTGCGCCGCGGGATGATCCCCGCGCACATCTACAACGACCCCGAACTGTTCGCGCTGGA
GAAGCGGCGGCTGTTCGCCCGGGCGTGGACGTTCGTGGGGCACGAGTCCGAGATTCCGCACGACGGCGACTACATGGTGC
GCCGGGTGCTCGACGATTCGTTCATCATCACCCGCGGATCCGACGGCCGGGTGCGGGCCGTGTTCAACATGTGCCTGCAC
CGCGGGATGCAGGTATGCCGCGCGGAGCTGGGCAATGCCTCCAATTTCCGCTGCCCGTATCACGGCTGGACCTACCGCAA
CGACGGCCGGCTCACCGGGTTACCGTTCCACCGGGAGGCCTACGGCGGCGACGACGGGTTCGTCAAGGACCAAACCCTTT
TGCCCGCACCGAATTTCGCCGGCTACGACGGGCTGCTGTTCATCAGCCTGGATCCGGCCGCACCACCGCTGCAGGATTTT
CTCGGCGACTTCCGGTTCTACCTGGACTACTACACCCGCCAAAGCGCGGGCGGCGTGGAGCTGCGCGGGCCGCAGCGCTG
GCGGATCCGGGCGAACTGGAAGATCGGGGCGGAGAATTTCGCTGGCGACATGTACCACACCCCGCACACCCACGCCTCGA
TCGTCGACATCGGGCTGTTCCGCGAGCCGAAGGCCCAGAAGCGCAAGGACGGCGCCACCTATTGGGCGCACCGCGGCGGC
GGCACCACCTACAAGCTGCCGCCGGGCGGCTTCGAGGAGCGGATGCGCTACGTCGGCTACCCCGACGAGATGATCGGGCG
GATCAAACAGGTGTGGACACCGCGGCAGCAGCGGGTGGTCGGCGAGGACGGGTTCATGGTCTCGGCGGCGACCTGCTTTC
CCAACCTGAGTTTCGTGCACAACTGGCCCAAGGTGCGCGACGGGCGCGACGACGAGACGCTGCCGTTCATCTCGATCCGG
TTGTGGCAGCCGATCAGCGAGAACGAGACCGAGGTGTGTTCGTGGTTCGCGGTGGACTCCGCGGCTCCCGCGCAGTACAA
ACAGGATTCGTACAAGGCGTATCTGATGTGCTTCGGGTCCACCGGCATGTTCGAGCAGGATGATGTGGAGAACTGGGTGT
CGCTGACCACCACCGCGGGCGGGTCGATGGCGCGGCGGCTGCTGCTGAACAGCCGGATGGGCCTGTACGACGACGGCCGC
CCGGTCGTCGAGGCGCTGGCGCCCAGCGCTTTCCACGGCCCCGGACGCGCGCAGGTCGGCTACAACGAGCACAACCAGCG
GGCGCTGCTGGCCATGTGGGCCGACTATCTGCAGGAAGGCGACGCATGCGAAAACCGTTGA

Upstream 100 bases:

>100_bases
GGGGGCCCGGAGATTGACCAGCGCTACCGACTGCGGGATTCTACTGAGCGGAAAGTATTTCTGCAGAACAGAGGCAGCGA
TGGCGGGGCCGGACGGCGGA

Downstream 100 bases:

>100_bases
CCTGCCTGCCCTTTGACGATGCTCGGCACCTGCAGGCGCACCAGTTCCTGGTGGACGAGGCCTACCTGCTCGACGCCCAG
CACTACCAGGCGTGGCTGGA

Product: hypothetical protein

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MPGVLENVRRGMIPAHIYNDPELFALEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDDSFIITRGSDGRVRAVFNMCLH
RGMQVCRAELGNASNFRCPYHGWTYRNDGRLTGLPFHREAYGGDDGFVKDQTLLPAPNFAGYDGLLFISLDPAAPPLQDF
LGDFRFYLDYYTRQSAGGVELRGPQRWRIRANWKIGAENFAGDMYHTPHTHASIVDIGLFREPKAQKRKDGATYWAHRGG
GTTYKLPPGGFEERMRYVGYPDEMIGRIKQVWTPRQQRVVGEDGFMVSAATCFPNLSFVHNWPKVRDGRDDETLPFISIR
LWQPISENETEVCSWFAVDSAAPAQYKQDSYKAYLMCFGSTGMFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDGR
PVVEALAPSAFHGPGRAQVGYNEHNQRALLAMWADYLQEGDACENR

Sequences:

>Translated_446_residues
MPGVLENVRRGMIPAHIYNDPELFALEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDDSFIITRGSDGRVRAVFNMCLH
RGMQVCRAELGNASNFRCPYHGWTYRNDGRLTGLPFHREAYGGDDGFVKDQTLLPAPNFAGYDGLLFISLDPAAPPLQDF
LGDFRFYLDYYTRQSAGGVELRGPQRWRIRANWKIGAENFAGDMYHTPHTHASIVDIGLFREPKAQKRKDGATYWAHRGG
GTTYKLPPGGFEERMRYVGYPDEMIGRIKQVWTPRQQRVVGEDGFMVSAATCFPNLSFVHNWPKVRDGRDDETLPFISIR
LWQPISENETEVCSWFAVDSAAPAQYKQDSYKAYLMCFGSTGMFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDGR
PVVEALAPSAFHGPGRAQVGYNEHNQRALLAMWADYLQEGDACENR
>Mature_445_residues
PGVLENVRRGMIPAHIYNDPELFALEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDDSFIITRGSDGRVRAVFNMCLHR
GMQVCRAELGNASNFRCPYHGWTYRNDGRLTGLPFHREAYGGDDGFVKDQTLLPAPNFAGYDGLLFISLDPAAPPLQDFL
GDFRFYLDYYTRQSAGGVELRGPQRWRIRANWKIGAENFAGDMYHTPHTHASIVDIGLFREPKAQKRKDGATYWAHRGGG
TTYKLPPGGFEERMRYVGYPDEMIGRIKQVWTPRQQRVVGEDGFMVSAATCFPNLSFVHNWPKVRDGRDDETLPFISIRL
WQPISENETEVCSWFAVDSAAPAQYKQDSYKAYLMCFGSTGMFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDGRP
VVEALAPSAFHGPGRAQVGYNEHNQRALLAMWADYLQEGDACENR

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=451, Percent_Identity=31.7073170731707, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788103, Length=208, Percent_Identity=33.1730769230769, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 50669; Mature: 50537

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGVLENVRRGMIPAHIYNDPELFALEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDDS
CCCHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
FIITRGSDGRVRAVFNMCLHRGMQVCRAELGNASNFRCPYHGWTYRNDGRLTGLPFHREA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEECCCCCHHH
YGGDDGFVKDQTLLPAPNFAGYDGLLFISLDPAAPPLQDFLGDFRFYLDYYTRQSAGGVE
CCCCCCCCCCCEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
LRGPQRWRIRANWKIGAENFAGDMYHTPHTHASIVDIGLFREPKAQKRKDGATYWAHRGG
ECCCCEEEEEEEEEECCHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHCCCCCEEEECCC
GTTYKLPPGGFEERMRYVGYPDEMIGRIKQVWTPRQQRVVGEDGFMVSAATCFPNLSFVH
CEEEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHEEECCCCCEEEEHHHCCCCHHHC
NWPKVRDGRDDETLPFISIRLWQPISENETEVCSWFAVDSAAPAQYKQDSYKAYLMCFGS
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCCEEEEEEECC
TGMFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDGRPVVEALAPSAFHGPGRAQVG
CCCCCCCCHHHHEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
YNEHNQRALLAMWADYLQEGDACENR
CCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PGVLENVRRGMIPAHIYNDPELFALEKRRLFARAWTFVGHESEIPHDGDYMVRRVLDDS
CCHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
FIITRGSDGRVRAVFNMCLHRGMQVCRAELGNASNFRCPYHGWTYRNDGRLTGLPFHREA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCEEECCCCCHHH
YGGDDGFVKDQTLLPAPNFAGYDGLLFISLDPAAPPLQDFLGDFRFYLDYYTRQSAGGVE
CCCCCCCCCCCEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
LRGPQRWRIRANWKIGAENFAGDMYHTPHTHASIVDIGLFREPKAQKRKDGATYWAHRGG
ECCCCEEEEEEEEEECCHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHCCCCCEEEECCC
GTTYKLPPGGFEERMRYVGYPDEMIGRIKQVWTPRQQRVVGEDGFMVSAATCFPNLSFVH
CEEEECCCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHEEECCCCCEEEEHHHCCCCHHHC
NWPKVRDGRDDETLPFISIRLWQPISENETEVCSWFAVDSAAPAQYKQDSYKAYLMCFGS
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCCEEEEEEECC
TGMFEQDDVENWVSLTTTAGGSMARRLLLNSRMGLYDDGRPVVEALAPSAFHGPGRAQVG
CCCCCCCCHHHHEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
YNEHNQRALLAMWADYLQEGDACENR
CCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1569021; 7592331 [H]