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The map label for this gene is ppdK

Identifier: 41408762

GI number: 41408762

Start: 3001086

End: 3002768

Strand: Direct

Name: ppdK

Synonym: MAP2664

Alternate gene names: 41408762

Gene position: 3001086-3002768 (Clockwise)

Preceding gene: 41408759

Following gene: 41408763

Centisome position: 62.14

GC content: 72.96

Gene sequence:

>1683_bases
TTGCCCGAATCAGCAGCGACACCACGAAGGGGGTGCACCACGGCTCGCACCGCACCAGGTAGCGGCGCCCACCGCAACGC
GTCACGCGACCGGGACGCCGCGCCGACCGAGCAGTCCGTGGTGTTGCTGGACGGCACATCGAGCCACCCCCGCGAGCTGC
TTGGCAACAAGGGCCACGGCATCGAGGTGATGCGCCGCCACCACCTGCCGGTGCCGCCCGCGTTCTGCCTCACCACCGCG
GTGGGCCTGCGGTATCTGGCCGACCCCGCGGCGACGATGGACGCCGTCTGGGACGACGTGCTGGACCGGATCGGCTGGCT
GCAGGCGCAAACCTCGCGCACGTTCGGGCGGGGCCCGCACCCGCTGCTGGTCAGCGTGCGCTCGGGGGCCACCCAGTCGA
TGCCCGGGATGATGGACACCCTGTTGAACCTCGGCATCAACGACTCCGTGGAGCAGGCGCTGGCCGCCGAGCACGGGCCG
GCGTTCGCCCGCGACACCCACCGGCGGTTCCGCGAGATGTACCGGCGCATCGTGGGTGTCGAGCGGCCGGTGCCGTCCGA
CCCGTACACGCAGTTGCGCGCCGGCATCGAGGCGGTGTTCGCGTCGTGGAACTCCCCGCGCGCCCGCGCCTACCGCGCCC
ATTACGGTTTCGCCGACCGGCACGGCACCGCCGTCGTCGTGCAGGCCATGGTGTTCGGCAATCACGGCCCCAATTCCGGT
GCGGGAGCGTTCTTTTCGCGCAACCCGATCACCGGGGACGACGAGCCGTTCGGCGAGTGGCTGCCCGGGGGCCAGGGCGA
CGACGTGGTGTCGGGATCGGTCGACGTGGAACCGATCGCCGCGCTGCGCGACGAGCAGCCGGCCGTCTACGACCAGCTGA
TGGCCGCCGCCCGGACGCTGGAGCGGTTGGACCGTGACGTCCAGGAGATCGAGTTCACCGTCGAGGACGGCGCCCTGTGG
TTGCTGCAGACCCGGGCGGCCGAACGCTCGGCGCAGGCGGCGGTGCGCTCGGCGCTGCAGCTGCGACGCGAGGGGCTGAT
CGACGACGCCGAGACGCTGCGGCGGGTCACCCCGGCGCACGTGCGGTCGCTGCTGATGCCCGCCCTGCAACCCGAAATAC
GTTTGGCGGCACCACTTCTGGCCAAGGGCCTACCTGCCTGCCCCGGCGTGGTATCCGGCACGGCCTACGCCGACGTCGAC
GAGGCGCTGCACGCCGCCGAGCGGGGCGAACCGGTGATCCTGGTGCGCGACCACACCCGGCCCGAAGACGTGTCGGGCAT
GCTGGCCGCGCGGGGCATCGTCACCGAGGTGGGCGGCGCGGCCAGCCATGCGGCGGTGGTCAGCCGGGAACTGGGCCGGG
TGGCCGTGGTGGGTTGCGGCGCCGGGGTGGCCGCGATGCTGGCGGGCCGGCGGATCACCGTCGACGGCGCCGAAGGCGAA
GTGCGCGAAGGCGAATTGTCCCTGTCCGCATGGTCCGAGAACGATACCCCGGAGCTGCGGGAGCTGGCCGAGATCGCGCG
GCTGATCAGCCCGCTGCGCGCCCATTCCGGCGGCGACCATCCGCGGCTGGACGACGACTCCGAGAGCGCCGTGCGCGCGG
CCCTGGCCGCCGGGCAGACCGACGTGGTCTCGCCCACCCCGCTGCTGGTCATGCTGACCGCGCTGCGCCAGGGCGCGCCG
TGA

Upstream 100 bases:

>100_bases
TAGGTGCAAACCTAGATCATCGGCCGCCGGGGAGGACAGGACCGCCGCGCGGCTGAGTCCACCCGCCGGCCCGTGTGGGA
ACATAGCGGGCGTTCCGGGG

Downstream 100 bases:

>100_bases
CCGAACTGGCTGTGCTGCAAGCGATTCGGCTCAAGGGCCGGGTCAGCCGAGCCGACCTGGCCGCCACCCTGGGCACGGAT
CCCGACGAAATCGCCGGCAC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MPESAATPRRGCTTARTAPGSGAHRNASRDRDAAPTEQSVVLLDGTSSHPRELLGNKGHGIEVMRRHHLPVPPAFCLTTA
VGLRYLADPAATMDAVWDDVLDRIGWLQAQTSRTFGRGPHPLLVSVRSGATQSMPGMMDTLLNLGINDSVEQALAAEHGP
AFARDTHRRFREMYRRIVGVERPVPSDPYTQLRAGIEAVFASWNSPRARAYRAHYGFADRHGTAVVVQAMVFGNHGPNSG
AGAFFSRNPITGDDEPFGEWLPGGQGDDVVSGSVDVEPIAALRDEQPAVYDQLMAAARTLERLDRDVQEIEFTVEDGALW
LLQTRAAERSAQAAVRSALQLRREGLIDDAETLRRVTPAHVRSLLMPALQPEIRLAAPLLAKGLPACPGVVSGTAYADVD
EALHAAERGEPVILVRDHTRPEDVSGMLAARGIVTEVGGAASHAAVVSRELGRVAVVGCGAGVAAMLAGRRITVDGAEGE
VREGELSLSAWSENDTPELRELAEIARLISPLRAHSGGDHPRLDDDSESAVRAALAAGQTDVVSPTPLLVMLTALRQGAP

Sequences:

>Translated_560_residues
MPESAATPRRGCTTARTAPGSGAHRNASRDRDAAPTEQSVVLLDGTSSHPRELLGNKGHGIEVMRRHHLPVPPAFCLTTA
VGLRYLADPAATMDAVWDDVLDRIGWLQAQTSRTFGRGPHPLLVSVRSGATQSMPGMMDTLLNLGINDSVEQALAAEHGP
AFARDTHRRFREMYRRIVGVERPVPSDPYTQLRAGIEAVFASWNSPRARAYRAHYGFADRHGTAVVVQAMVFGNHGPNSG
AGAFFSRNPITGDDEPFGEWLPGGQGDDVVSGSVDVEPIAALRDEQPAVYDQLMAAARTLERLDRDVQEIEFTVEDGALW
LLQTRAAERSAQAAVRSALQLRREGLIDDAETLRRVTPAHVRSLLMPALQPEIRLAAPLLAKGLPACPGVVSGTAYADVD
EALHAAERGEPVILVRDHTRPEDVSGMLAARGIVTEVGGAASHAAVVSRELGRVAVVGCGAGVAAMLAGRRITVDGAEGE
VREGELSLSAWSENDTPELRELAEIARLISPLRAHSGGDHPRLDDDSESAVRAALAAGQTDVVSPTPLLVMLTALRQGAP
>Mature_559_residues
PESAATPRRGCTTARTAPGSGAHRNASRDRDAAPTEQSVVLLDGTSSHPRELLGNKGHGIEVMRRHHLPVPPAFCLTTAV
GLRYLADPAATMDAVWDDVLDRIGWLQAQTSRTFGRGPHPLLVSVRSGATQSMPGMMDTLLNLGINDSVEQALAAEHGPA
FARDTHRRFREMYRRIVGVERPVPSDPYTQLRAGIEAVFASWNSPRARAYRAHYGFADRHGTAVVVQAMVFGNHGPNSGA
GAFFSRNPITGDDEPFGEWLPGGQGDDVVSGSVDVEPIAALRDEQPAVYDQLMAAARTLERLDRDVQEIEFTVEDGALWL
LQTRAAERSAQAAVRSALQLRREGLIDDAETLRRVTPAHVRSLLMPALQPEIRLAAPLLAKGLPACPGVVSGTAYADVDE
ALHAAERGEPVILVRDHTRPEDVSGMLAARGIVTEVGGAASHAAVVSRELGRVAVVGCGAGVAAMLAGRRITVDGAEGEV
REGELSLSAWSENDTPELRELAEIARLISPLRAHSGGDHPRLDDDSESAVRAALAAGQTDVVSPTPLLVMLTALRQGAP

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=305, Percent_Identity=28.1967213114754, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 59739; Mature: 59608

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESAATPRRGCTTARTAPGSGAHRNASRDRDAAPTEQSVVLLDGTSSHPRELLGNKGHG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCC
IEVMRRHHLPVPPAFCLTTAVGLRYLADPAATMDAVWDDVLDRIGWLQAQTSRTFGRGPH
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PLLVSVRSGATQSMPGMMDTLLNLGINDSVEQALAAEHGPAFARDTHRRFREMYRRIVGV
CEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ERPVPSDPYTQLRAGIEAVFASWNSPRARAYRAHYGFADRHGTAVVVQAMVFGNHGPNSG
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
AGAFFSRNPITGDDEPFGEWLPGGQGDDVVSGSVDVEPIAALRDEQPAVYDQLMAAARTL
CCCCEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHH
ERLDRDVQEIEFTVEDGALWLLQTRAAERSAQAAVRSALQLRREGLIDDAETLRRVTPAH
HHHHHHHHHHEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHH
VRSLLMPALQPEIRLAAPLLAKGLPACPGVVSGTAYADVDEALHAAERGEPVILVRDHTR
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
PEDVSGMLAARGIVTEVGGAASHAAVVSRELGRVAVVGCGAGVAAMLAGRRITVDGAEGE
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHCCCEEEEECCCCC
VREGELSLSAWSENDTPELRELAEIARLISPLRAHSGGDHPRLDDDSESAVRAALAAGQT
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
DVVSPTPLLVMLTALRQGAP
CCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
PESAATPRRGCTTARTAPGSGAHRNASRDRDAAPTEQSVVLLDGTSSHPRELLGNKGHG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHCCCCCC
IEVMRRHHLPVPPAFCLTTAVGLRYLADPAATMDAVWDDVLDRIGWLQAQTSRTFGRGPH
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PLLVSVRSGATQSMPGMMDTLLNLGINDSVEQALAAEHGPAFARDTHRRFREMYRRIVGV
CEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
ERPVPSDPYTQLRAGIEAVFASWNSPRARAYRAHYGFADRHGTAVVVQAMVFGNHGPNSG
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
AGAFFSRNPITGDDEPFGEWLPGGQGDDVVSGSVDVEPIAALRDEQPAVYDQLMAAARTL
CCCCEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHH
ERLDRDVQEIEFTVEDGALWLLQTRAAERSAQAAVRSALQLRREGLIDDAETLRRVTPAH
HHHHHHHHHHEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHH
VRSLLMPALQPEIRLAAPLLAKGLPACPGVVSGTAYADVDEALHAAERGEPVILVRDHTR
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
PEDVSGMLAARGIVTEVGGAASHAAVVSRELGRVAVVGCGAGVAAMLAGRRITVDGAEGE
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHCCCEEEEECCCCC
VREGELSLSAWSENDTPELRELAEIARLISPLRAHSGGDHPRLDDDSESAVRAALAAGQT
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
DVVSPTPLLVMLTALRQGAP
CCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]