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The map label for this gene is metE

Identifier: 41408759

GI number: 41408759

Start: 2996241

End: 2998511

Strand: Direct

Name: metE

Synonym: MAP2661

Alternate gene names: 41408759

Gene position: 2996241-2998511 (Clockwise)

Preceding gene: 41408758

Following gene: 41408762

Centisome position: 62.04

GC content: 70.63

Gene sequence:

>2271_bases
GTGACCCCTCAAGCATTCACCGCAACGGTTGTCGGATCGCCGCGCATCGGGCCGAAGCGCGAACTCAAGCGCGCCACCGA
GGGCTACTGGGCCGGCCGCACCGGTCGCGCCGAGCTCGAAAAGGTGGCCGCGACGCTGCGCCGCGACACCTGGGCGGGCC
TGGCGGCCGCCGGCCTGGACTCGGTGCCGGTGAACACCTTCTCCTATTACGACCAGATGCTCGACACCGCGGTGATGCTC
GACGCGCTGCCGGAGCGGGCCCGGCAGGTGTCCGACGACCTGGATCGCTACTTCGCGGCCGCCCGCGGCAACTCCGACGT
CGCACCGCTGGAGATGACCAAGTGGTTCGACACCAACTACCACTACATCGTCCCCGAGATCGCGCCCACCACCAAGTTCG
CGCTGAACCCCGACAAGGTGCTTTCGGAGCTGAAAGAAGCTCTGGCACAAGGTATTCCGGCCCGGCCTGTGGTCATCGGG
CCGATCACCTTCCTGCTGCTCAGCAAGGGCGTCGACGGGGCGGGCGCCCCCATCGAGCGGCTCGAGGAGCTGGTGCCGAT
CTACGCCGAGCTGCTGTCGCTGCTCGCCGACAACGGGGCGCAGTGGGTGCAGCTCGACGAGCCGGCGCTGGTGACCGACA
TGTGCGCCGACGCGCCCGCCCTGGCCGAGGCGGTCTACAACAAGCTGGGCTCGGCAAGCAACCGGCCGGCCATCTACGTC
GCCACCTACTTCGGTGACCCGGGGGCGTCGCTGGCCGGGCTGGCCCGCACGCCTGTCGAGGCGATCGGCGTCGACCTGGT
GTACGGCCCCGACACCGCCGTCGCCGCCGTGCCCGAGCTGGCCGGCAAGACCGTGGTGGCCGGCGTGGTCGACGGCCGCA
ACGTGTGGCGCACCGACCTGGCGGCGGCGCTGGACAAGCTGACCACGCTGCTGGGTTCGGCAGCGCGCGTTGCGGTTTCG
ACGTCGTGCTCCACGCTGCACGTGCCCTACTCGCTGGAGCCGGAGACCGACCTGGACGACGCGCTGCGCAGCTGGCTGGC
GTTCGGGCGGGAGAAGGTCGCCGAGGTGGTGACGCTGGCGCGTGCCCTGCGCGACGGGCGCGAGGCCGTCGCCGACGAGA
TCGCGGCGTCCAACGCGGCCGTCGCCTCCCGCAAGAGCGACCCCCGCCTGCACAACGACCGCGTCCGGGCCCGCATCGAC
TCGATCGTCGCGTCTGGTTCGCACCGCGGCGACCCGGCCCAGCGCCGCGCCAGCCAGGACGCGCGGCTGCACCTGCCGCC
GCTGCCCACCACGACCATCGGCTCCTACCCGCAGACCTCGGCAATCCGCAAGGCGCGCGCGGCATTACGCTCCGGCGAGA
TCGACCAGGCCGAGTACGAGAGGCGGATGAAAAAGGAGATCGCCGACGTCATCACGCTGCAGGAGCAGCTCGGCCTCGAC
GTGTTGGTGCACGGCGAGCCGGAGCGCAACGACATGGTGCAGTACTTCGCCGAGCAGTTGGAGGGCTTCTTCGCGACGCA
GAACGGCTGGGTGCAGTCCTACGGCAGCCGCTGCGTGCGGCCGCCGATCCTGTACGGCGATGTGGTGCGCACCCACCCGA
TGACGGTCGAGTGGATCAGCTACGCGCAGTCGCTGACCGACAAGCCGGTGAAGGGCATGCTGACCGGGCCGGTGACGATC
CTGGCGTGGTCGTTCGTCCGCGACGACCAGCCGCTGGCCGACACCGCCAACCAGGTGGCGCTGGCCATCCGCGACGAGAC
GGTGGACCTGCAGGCGGCGGGCATCGCGGTCATCCAGGTCGACGAGCCGGCGCTGCGCGAGCTGTTGCCGCTGCGCCGGG
CCGATCAGGACGAGTATTTGCGTTGGGCCGTAGGCGCTTTCCGGCTGGCCACCTCCGGGGTCGCCGACTCGACCCAGATC
CACACCCATCTGTGCTACTCGGAGTTCGGTGAGGTGATCGGGGCGATCGCCGACCTGGACGCCGACGTGACCTCCCTGGA
GGCCGCCCGCTCGCACATGGAGGTGCTCGACGACCTGAACGCGGTCGGCTTCTCCAACAGCGTCGGGCCGGGGGTCTACG
ACATCCACTCGCCGCGGGTGCCGAGCACCGCCGAGATCGCCGAGTCGTTGCGCGCGGCGCTGCGGGCGGTGCCGGCCGAG
CGGTTGTGGGTCAACCCCGACTGCGGGCTCAAGACCCGCAACCCCGACGAGGTGAGCGCGTCGCTGAAGAACATGGTCGC
CGCGGCCCACGAGGTTCGGGCCGGGGTCTGA

Upstream 100 bases:

>100_bases
TTGGCTGCAGCCGTTGCGGTCGGTACCGGGTACCCACCGCCGACGGGTCGGCTGCACGTCCGCGGGCGGTGACCACCACG
CCGATTGAAGGACGAATATC

Downstream 100 bases:

>100_bases
GCCGCCGAAGCCCGAGCGTCACGCCAGCGTGACGCTCGGGCTTCGGCGTCACTCTGGCGTGACGCTCGGCGCGTCGTCCG
ACACCACCTGCACGGGGACG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 756; Mature: 755

Protein sequence:

>756_residues
MTPQAFTATVVGSPRIGPKRELKRATEGYWAGRTGRAELEKVAATLRRDTWAGLAAAGLDSVPVNTFSYYDQMLDTAVML
DALPERARQVSDDLDRYFAAARGNSDVAPLEMTKWFDTNYHYIVPEIAPTTKFALNPDKVLSELKEALAQGIPARPVVIG
PITFLLLSKGVDGAGAPIERLEELVPIYAELLSLLADNGAQWVQLDEPALVTDMCADAPALAEAVYNKLGSASNRPAIYV
ATYFGDPGASLAGLARTPVEAIGVDLVYGPDTAVAAVPELAGKTVVAGVVDGRNVWRTDLAAALDKLTTLLGSAARVAVS
TSCSTLHVPYSLEPETDLDDALRSWLAFGREKVAEVVTLARALRDGREAVADEIAASNAAVASRKSDPRLHNDRVRARID
SIVASGSHRGDPAQRRASQDARLHLPPLPTTTIGSYPQTSAIRKARAALRSGEIDQAEYERRMKKEIADVITLQEQLGLD
VLVHGEPERNDMVQYFAEQLEGFFATQNGWVQSYGSRCVRPPILYGDVVRTHPMTVEWISYAQSLTDKPVKGMLTGPVTI
LAWSFVRDDQPLADTANQVALAIRDETVDLQAAGIAVIQVDEPALRELLPLRRADQDEYLRWAVGAFRLATSGVADSTQI
HTHLCYSEFGEVIGAIADLDADVTSLEAARSHMEVLDDLNAVGFSNSVGPGVYDIHSPRVPSTAEIAESLRAALRAVPAE
RLWVNPDCGLKTRNPDEVSASLKNMVAAAHEVRAGV

Sequences:

>Translated_756_residues
MTPQAFTATVVGSPRIGPKRELKRATEGYWAGRTGRAELEKVAATLRRDTWAGLAAAGLDSVPVNTFSYYDQMLDTAVML
DALPERARQVSDDLDRYFAAARGNSDVAPLEMTKWFDTNYHYIVPEIAPTTKFALNPDKVLSELKEALAQGIPARPVVIG
PITFLLLSKGVDGAGAPIERLEELVPIYAELLSLLADNGAQWVQLDEPALVTDMCADAPALAEAVYNKLGSASNRPAIYV
ATYFGDPGASLAGLARTPVEAIGVDLVYGPDTAVAAVPELAGKTVVAGVVDGRNVWRTDLAAALDKLTTLLGSAARVAVS
TSCSTLHVPYSLEPETDLDDALRSWLAFGREKVAEVVTLARALRDGREAVADEIAASNAAVASRKSDPRLHNDRVRARID
SIVASGSHRGDPAQRRASQDARLHLPPLPTTTIGSYPQTSAIRKARAALRSGEIDQAEYERRMKKEIADVITLQEQLGLD
VLVHGEPERNDMVQYFAEQLEGFFATQNGWVQSYGSRCVRPPILYGDVVRTHPMTVEWISYAQSLTDKPVKGMLTGPVTI
LAWSFVRDDQPLADTANQVALAIRDETVDLQAAGIAVIQVDEPALRELLPLRRADQDEYLRWAVGAFRLATSGVADSTQI
HTHLCYSEFGEVIGAIADLDADVTSLEAARSHMEVLDDLNAVGFSNSVGPGVYDIHSPRVPSTAEIAESLRAALRAVPAE
RLWVNPDCGLKTRNPDEVSASLKNMVAAAHEVRAGV
>Mature_755_residues
TPQAFTATVVGSPRIGPKRELKRATEGYWAGRTGRAELEKVAATLRRDTWAGLAAAGLDSVPVNTFSYYDQMLDTAVMLD
ALPERARQVSDDLDRYFAAARGNSDVAPLEMTKWFDTNYHYIVPEIAPTTKFALNPDKVLSELKEALAQGIPARPVVIGP
ITFLLLSKGVDGAGAPIERLEELVPIYAELLSLLADNGAQWVQLDEPALVTDMCADAPALAEAVYNKLGSASNRPAIYVA
TYFGDPGASLAGLARTPVEAIGVDLVYGPDTAVAAVPELAGKTVVAGVVDGRNVWRTDLAAALDKLTTLLGSAARVAVST
SCSTLHVPYSLEPETDLDDALRSWLAFGREKVAEVVTLARALRDGREAVADEIAASNAAVASRKSDPRLHNDRVRARIDS
IVASGSHRGDPAQRRASQDARLHLPPLPTTTIGSYPQTSAIRKARAALRSGEIDQAEYERRMKKEIADVITLQEQLGLDV
LVHGEPERNDMVQYFAEQLEGFFATQNGWVQSYGSRCVRPPILYGDVVRTHPMTVEWISYAQSLTDKPVKGMLTGPVTIL
AWSFVRDDQPLADTANQVALAIRDETVDLQAAGIAVIQVDEPALRELLPLRRADQDEYLRWAVGAFRLATSGVADSTQIH
THLCYSEFGEVIGAIADLDADVTSLEAARSHMEVLDDLNAVGFSNSVGPGVYDIHSPRVPSTAEIAESLRAALRAVPAER
LWVNPDCGLKTRNPDEVSASLKNMVAAAHEVRAGV

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=753, Percent_Identity=46.8791500664011, Blast_Score=676, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=769, Percent_Identity=46.814044213264, Blast_Score=646, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 81592; Mature: 81461

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQAFTATVVGSPRIGPKRELKRATEGYWAGRTGRAELEKVAATLRRDTWAGLAAAGLD
CCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SVPVNTFSYYDQMLDTAVMLDALPERARQVSDDLDRYFAAARGNSDVAPLEMTKWFDTNY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCC
HYIVPEIAPTTKFALNPDKVLSELKEALAQGIPARPVVIGPITFLLLSKGVDGAGAPIER
CEEECCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCHHH
LEELVPIYAELLSLLADNGAQWVQLDEPALVTDMCADAPALAEAVYNKLGSASNRPAIYV
HHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEE
ATYFGDPGASLAGLARTPVEAIGVDLVYGPDTAVAAVPELAGKTVVAGVVDGRNVWRTDL
EEEECCCCCCHHHHHHCCHHHHCEEEEECCCHHHHHHHHHCCCEEEEEECCCCHHHHHHH
AAALDKLTTLLGSAARVAVSTSCSTLHVPYSLEPETDLDDALRSWLAFGREKVAEVVTLA
HHHHHHHHHHHCCHHHEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RALRDGREAVADEIAASNAAVASRKSDPRLHNDRVRARIDSIVASGSHRGDPAQRRASQD
HHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC
ARLHLPPLPTTTIGSYPQTSAIRKARAALRSGEIDQAEYERRMKKEIADVITLQEQLGLD
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VLVHGEPERNDMVQYFAEQLEGFFATQNGWVQSYGSRCVRPPILYGDVVRTHPMTVEWIS
EEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEECCHHHCCCCHHHHHH
YAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQAAGIAVIQV
HHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHEEEEEECCCEEEEECCEEEEEE
DEPALRELLPLRRADQDEYLRWAVGAFRLATSGVADSTQIHTHLCYSEFGEVIGAIADLD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
ADVTSLEAARSHMEVLDDLNAVGFSNSVGPGVYDIHSPRVPSTAEIAESLRAALRAVPAE
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHH
RLWVNPDCGLKTRNPDEVSASLKNMVAAAHEVRAGV
HEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPQAFTATVVGSPRIGPKRELKRATEGYWAGRTGRAELEKVAATLRRDTWAGLAAAGLD
CCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SVPVNTFSYYDQMLDTAVMLDALPERARQVSDDLDRYFAAARGNSDVAPLEMTKWFDTNY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCC
HYIVPEIAPTTKFALNPDKVLSELKEALAQGIPARPVVIGPITFLLLSKGVDGAGAPIER
CEEECCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCHHH
LEELVPIYAELLSLLADNGAQWVQLDEPALVTDMCADAPALAEAVYNKLGSASNRPAIYV
HHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEE
ATYFGDPGASLAGLARTPVEAIGVDLVYGPDTAVAAVPELAGKTVVAGVVDGRNVWRTDL
EEEECCCCCCHHHHHHCCHHHHCEEEEECCCHHHHHHHHHCCCEEEEEECCCCHHHHHHH
AAALDKLTTLLGSAARVAVSTSCSTLHVPYSLEPETDLDDALRSWLAFGREKVAEVVTLA
HHHHHHHHHHHCCHHHEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RALRDGREAVADEIAASNAAVASRKSDPRLHNDRVRARIDSIVASGSHRGDPAQRRASQD
HHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC
ARLHLPPLPTTTIGSYPQTSAIRKARAALRSGEIDQAEYERRMKKEIADVITLQEQLGLD
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VLVHGEPERNDMVQYFAEQLEGFFATQNGWVQSYGSRCVRPPILYGDVVRTHPMTVEWIS
EEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEECCHHHCCCCHHHHHH
YAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQAAGIAVIQV
HHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHEEEEEECCCEEEEECCEEEEEE
DEPALRELLPLRRADQDEYLRWAVGAFRLATSGVADSTQIHTHLCYSEFGEVIGAIADLD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
ADVTSLEAARSHMEVLDDLNAVGFSNSVGPGVYDIHSPRVPSTAEIAESLRAALRAVPAE
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCHH
RLWVNPDCGLKTRNPDEVSASLKNMVAAAHEVRAGV
HEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA