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The map label for this gene is lpxD

Identifier: 52843138

GI number: 52843138

Start: 3329878

End: 3330915

Strand: Direct

Name: lpxD

Synonym: lpg2944

Alternate gene names: 52843138

Gene position: 3329878-3330915 (Clockwise)

Preceding gene: 52843137

Following gene: 52843145

Centisome position: 98.0

GC content: 42.77

Gene sequence:

>1038_bases
GTGTGTATGTCAAATTACCAATTTACAAAACCTGCAGGACCATTTCGTTTATTCGAATTAGCTAAGATTTCTGGTGCTAC
ACTGTATGAAGGTAAAGGAGAAACCTTTACAGTTTCTGGTTTGGCAAAACTTTCAGAAGCCACAAGCAATGATTTGGTAA
TGCTGCATCAAAAAAAATACGTCAAAGAATTAAAGCATACAGCTGCTCGTGTTTGTATCATCGGGCCAGACTATGTGAAA
TATGCCCCTGATAGCATGTATTTACTGGTGCACCCCAATCCCTACAAAGCATTTGCTTTGATTGCTCAAGCGTTTTATCC
TTCTGAAAAACCACCCGGTTTCATTGCCACTTCGGCAATGATTGAGTCGAGCGCTATTATTGGCGTGGATTGTTTTATTG
CTCATGGGGCTTATATTGGCAATCAGGTTAAAATTGGCAACAGATGCAAAATTGGAGTGAATACTTATATTGGCGATACT
GTAACCATAGGCGATGATTGTCTTATAGAAGACAACGTCAGCATTAGGCATGCTGTTATAGGGAATAACGTGGTCATTTA
TTCCGGTGCTCGTATAGGCCAGGATGGTTTTGGCTTTGCCAGTGATGCGAATGGCCATTATAAAATTCCCCATGCGGGTG
GTGTCATTATTGGCAATGATGTTGAAATCGGGGCTAATACCTGCATTGACCGAGGCTCTCTGGATAATACGGTGATTGAA
GATTGGTGTCGTCTGGATAATCTTGTGCAGATTGGGCATAATGTCAAGATTGGTAAAGGCTCTGTCCTAGTGGCTCAAGT
TGGAATCGCCGGCAGTACAGAGCTTGGTGAACATGTCACTTTGGCAGGACAAGTTGGCGTGATTGGACACTTGAAGATCG
GTAAAGGCGCCACTGTTTTAGCCAGCGCTAAAGTCTATAAAAATGTGAAATCCGGAGACAGGGTCGGCGGCCACCCAGCG
GTATCAATTAGTGATTGGCAAAAACAAATCCGATTTTTAAAAACAGCGATTAAGTCAAAGAAGTCCCCAAAATCCTGA

Upstream 100 bases:

>100_bases
TGAAATACTCAAGCGTGAATTCATAGACAATAATGATGTGGCTTATATTCTCAAGTTTCTTGAAAATTCCAAACGCAGCT
TTTATCGCTAATTTTTTAGA

Downstream 100 bases:

>100_bases
GGATAGATAAAGCCGCTTTCTCATTAGCATTTTGCCTTAATGTGCAGTGAATATCAGTAAATTGTTTAATTAGTTCGTTT
TGATTGGATGAGTCGAGTAA

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MCMSNYQFTKPAGPFRLFELAKISGATLYEGKGETFTVSGLAKLSEATSNDLVMLHQKKYVKELKHTAARVCIIGPDYVK
YAPDSMYLLVHPNPYKAFALIAQAFYPSEKPPGFIATSAMIESSAIIGVDCFIAHGAYIGNQVKIGNRCKIGVNTYIGDT
VTIGDDCLIEDNVSIRHAVIGNNVVIYSGARIGQDGFGFASDANGHYKIPHAGGVIIGNDVEIGANTCIDRGSLDNTVIE
DWCRLDNLVQIGHNVKIGKGSVLVAQVGIAGSTELGEHVTLAGQVGVIGHLKIGKGATVLASAKVYKNVKSGDRVGGHPA
VSISDWQKQIRFLKTAIKSKKSPKS

Sequences:

>Translated_345_residues
MCMSNYQFTKPAGPFRLFELAKISGATLYEGKGETFTVSGLAKLSEATSNDLVMLHQKKYVKELKHTAARVCIIGPDYVK
YAPDSMYLLVHPNPYKAFALIAQAFYPSEKPPGFIATSAMIESSAIIGVDCFIAHGAYIGNQVKIGNRCKIGVNTYIGDT
VTIGDDCLIEDNVSIRHAVIGNNVVIYSGARIGQDGFGFASDANGHYKIPHAGGVIIGNDVEIGANTCIDRGSLDNTVIE
DWCRLDNLVQIGHNVKIGKGSVLVAQVGIAGSTELGEHVTLAGQVGVIGHLKIGKGATVLASAKVYKNVKSGDRVGGHPA
VSISDWQKQIRFLKTAIKSKKSPKS
>Mature_345_residues
MCMSNYQFTKPAGPFRLFELAKISGATLYEGKGETFTVSGLAKLSEATSNDLVMLHQKKYVKELKHTAARVCIIGPDYVK
YAPDSMYLLVHPNPYKAFALIAQAFYPSEKPPGFIATSAMIESSAIIGVDCFIAHGAYIGNQVKIGNRCKIGVNTYIGDT
VTIGDDCLIEDNVSIRHAVIGNNVVIYSGARIGQDGFGFASDANGHYKIPHAGGVIIGNDVEIGANTCIDRGSLDNTVIE
DWCRLDNLVQIGHNVKIGKGSVLVAQVGIAGSTELGEHVTLAGQVGVIGHLKIGKGATVLASAKVYKNVKSGDRVGGHPA
VSISDWQKQIRFLKTAIKSKKSPKS

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786376, Length=333, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573 [H]

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 36796; Mature: 36796

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCMSNYQFTKPAGPFRLFELAKISGATLYEGKGETFTVSGLAKLSEATSNDLVMLHQKKY
CCCCCCCCCCCCCCEEEEEEHHCCCCEEECCCCCEEEEHHHHHHHHCCCCCEEEEEHHHH
VKELKHTAARVCIIGPDYVKYAPDSMYLLVHPNPYKAFALIAQAFYPSEKPPGFIATSAM
HHHHHHHHEEEEEECCHHHEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEHHH
IESSAIIGVDCFIAHGAYIGNQVKIGNRCKIGVNTYIGDTVTIGDDCLIEDNVSIRHAVI
HCCCCEEEEEEEEECCCEECCEEEECCEEEEEEEEECCCEEEECCCEEEECCCCEEEEEE
GNNVVIYSGARIGQDGFGFASDANGHYKIPHAGGVIIGNDVEIGANTCIDRGSLDNTVIE
CCCEEEEECCEECCCCCCCCCCCCCCEECCCCCCEEECCCEEECCCCCCCCCCCCHHHHH
DWCRLDNLVQIGHNVKIGKGSVLVAQVGIAGSTELGEHVTLAGQVGVIGHLKIGKGATVL
HHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCEEEEEEECCCCEEE
ASAKVYKNVKSGDRVGGHPAVSISDWQKQIRFLKTAIKSKKSPKS
EHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCMSNYQFTKPAGPFRLFELAKISGATLYEGKGETFTVSGLAKLSEATSNDLVMLHQKKY
CCCCCCCCCCCCCCEEEEEEHHCCCCEEECCCCCEEEEHHHHHHHHCCCCCEEEEEHHHH
VKELKHTAARVCIIGPDYVKYAPDSMYLLVHPNPYKAFALIAQAFYPSEKPPGFIATSAM
HHHHHHHHEEEEEECCHHHEECCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEHHH
IESSAIIGVDCFIAHGAYIGNQVKIGNRCKIGVNTYIGDTVTIGDDCLIEDNVSIRHAVI
HCCCCEEEEEEEEECCCEECCEEEECCEEEEEEEEECCCEEEECCCEEEECCCCEEEEEE
GNNVVIYSGARIGQDGFGFASDANGHYKIPHAGGVIIGNDVEIGANTCIDRGSLDNTVIE
CCCEEEEECCEECCCCCCCCCCCCCCEECCCCCCEEECCCEEECCCCCCCCCCCCHHHHH
DWCRLDNLVQIGHNVKIGKGSVLVAQVGIAGSTELGEHVTLAGQVGVIGHLKIGKGATVL
HHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCEEEEEEECCCCEEE
ASAKVYKNVKSGDRVGGHPAVSISDWQKQIRFLKTAIKSKKSPKS
EHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA